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Citations for Bio-Rad :
3451 - 3500 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories, USA) with 2 mM ATP (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 95°C for 2 min and amplified for 24 cycles (95°C 15 seconds, 60°C, 4 min) on T100 PCR instrument (Biorad, Hercules, CA, USA). At the end of this process ...
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bioRxiv - Molecular Biology 2021Quote: ... and then liposomes were formed by the step-wise removal of pentaethylene glycol monodecyl ether by five additions at 20 min intervals of 60 mg Bio-Beads SM-2 (Bio-Rad, Watford, UK) with gentle mixing at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Round spermatids were sorted based on 1C DNA content and size using a 2-laser sorter (BioRad S3e with 488nm and 561nm lasers) gated on single cells as previously described (Lesch et al. ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were resolved by SDS-PAGE and transferred to a PVDF membrane by tank blotting at 150 mA for 2 h using the Miniprotean III System from Bio-Rad (Hercules, USA). The membrane was blocked for 1 h with 5% skim milk in PBS containing 0.05% Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38) before running on a CFX96TM real-time PCR cycler (BioRad, Hercules, CA, USA) under cycle conditions of 42°C for 5 min ...
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Plant Biology 2022Quote: ... 50 mg of powder was transferred into a 1.5 mL microcentrifuge tube with 150 ul of 2 x Laemmli buffer (Bio-Rad, Hercules, CA, USA). The sample was immediately vortexed for 1 min followed by incubation at 70°C for 10 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cervicornis sperm samples from 2015 were brought to a final concentration of 3 x 107 cells ml-1 after the addition of Cell Suspension Buffer and 2% agarose using the Bio-Rad CHEF Genomic DNA Plug Kits (Bio-Rad, Hercules, CA). Genomic DNA plugs were processed according to the manufacturer’s protocol and stored at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The assembled products were mixed with electrocompetent cells and transformed into electrocompetent cells in a 2-mm Gene Pulser cuvette (Bio-Rad, 165-2086) using an electroporation instrument (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Twenty µg (islet sample) or 2 µg (mouse liver sample: positive control) was mixed with 10uL of Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and denatured at 95°C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: RT-PCR was performed after generating first-strand cDNAs from 2 µg of total RNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA), as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (0.2 µm pore size) using the Trans-Blot® Turbo™ semi-dry transfer system (7 min mixed MW MIDI program) (Bio-Rad). After blocking in a 2.5% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: mMEC and hMEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Immunology 2023Quote: ... The amplified products were analyzed on a 2% agarose gel stained with SYBR safe and imaged using the Gel-documentation system (Bio-Rad, Hercules, CA).
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... and loaded (crude homogenate: 10 µg per lane; membrane: 2 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... Ten μg (final volume 2 μl for all samples) of serum proteins were loaded onto a nitrocellulose membrane (Bio-rad Laboratories, CA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were resolved on 1× TAE 1% agarose gels and imaged using Gel Doc EZ System (Bio-Rad). Raw gel scans are provided in SI Data File 4 ...
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bioRxiv - Microbiology 2019Quote: ... Ssp6-HA was detected using anti-HA primary antibody (MRC PPU Reagents, University of Dundee, 1:6,000) and HRP-conjugated anti-mouse secondary (1:10,000; Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... HRP (horseradish peroxidase)-conjugated goat anti-mouse (#176516, 1:10,000 for WB) and anti-rabbit IgG antibodies (#176515, 1:10,000 for WB) were from Bio-Rad. Alexa Fluor conjugated goat anti-mouse (1:500 for IF) ...
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bioRxiv - Microbiology 2020Quote: ... membranes were blocked overnight in PBST supplemented with 5% non-fat dry milk and incubated with antiserum (1:5,000) or vaginal washes (1:500) from test or control mice followed by goat anti-mouse IgG (BioRad) or IgA (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1 h at room temperature with peroxidase-conjugated anti-mouse (1:3000; BioRad, Hercules, CA) or anti-rabbit (1:3000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected with anti-rabbit (1:3,000) and anti-mouse (1:20,000) secondary antibodies coupled to horseradish peroxidase (Bio-Rad), diluted in 3% BSA-PBST ...
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bioRxiv - Biochemistry 2022Quote: ... the responses obtained from the AmQNS-small molecular interactions at different concentrations were fitted using the Langmuir 1:1 biomolecular interaction model using the ProteOn Manager software version 3.1.0.6 (Bio-Rad, USA). Equilibrium dissociation constants (KD ...
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bioRxiv - Microbiology 2020Quote: One ug of each respective protein was mixed at a 1:1 ratio with 2X Laemmli buffer (Bio-Rad) which was supplemented with 2% β-mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... or by anti-Smc3 K112/K113 Acetylation (1:1,000, gift from Dr. Katsuhiko Shirahige) in combination with goat anti-mouse HRP (1:10,000) (Bio-Rad) and ECL prime (GE Healthcare ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged to pellet tissue debris and equal volumes mixed 1:1 with 2x Laemmli sample buffer (BioRad). Samples were boiled ...
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bioRxiv - Biochemistry 2021Quote: ... was performed with 1:1,000 or 1:100,000 diluted PCR1 product using SsoAdvanced Universal SYBR green supermix (Bio-Rad). To quantify the Pten 5-6k isoform ...
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bioRxiv - Genetics 2019Quote: ... at 4° C with 100 V for 1 hour in 1 x Tris/Glycine Buffer (Bio-Rad, Cat#1610734) and 20% methanol (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GroEL was detected with anti-GroEL primary antibody (Pierce; 1:10,000) and anti-rabbit secondary antibody (Biorad; 1:20,000).
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bioRxiv - Bioengineering 2023Quote: ... A 10 μL aliquot of each assembly reaction was mixed 1:1 with Native Sample Buffer (Bio-Rad Laboratories), loaded into precast 4-15% polyacrylamide gels (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2023Quote: PFA fixed brain samples were incubated in 1% hydrogel monomers (HMs) consisting of 1% w/v acrylamide (#1610140, BioRad), 0.05% w/v bisacrylamide (#1610142 ...
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bioRxiv - Genomics 2022Quote: Antibodies and their working concentrations: anti-mouse (1:5000; #7076, Cell Signaling Technology, or 1:20000, #STAR117D800GA, Bio-Rad), anti-rabbit (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... The nitrocellulose membrane was exposed to a 1:1 v/v solution of Clarity Western ECL Substrate (Bio-rad) for 5 mins and chemiluminescence quantified and analysed using FluorChem E software (ProteinSimple).
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011 and 1:5,000 dilution, ref: 170-6515, Bio-Rad) in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: Approximately 1 × 105 bacterial cells were suspended in PBS containing 1% (w/v) PBS-BSA and incubated with fH (Biorad) at a final concentration of 5 μg/mL for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were washed three times for 5 min and incubated with either a 1:5000 (Mouse)/1:10000 (Rabbit) dilution of horseradish peroxidase-conjugated secondary antibodies or with fluorophore-conjugated 1:5000 dilution of Starbright700 (Biorad) for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated secondary antibodies were diluted 1:10000 and 1:2000 for polyclonal Goat-α-Mouse (Bio-Rad, 170-6516) and Goat-α-Rabbit HRP (Dako ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA at pH 8.3) and by pulse field gel electrophoresis in 1% Certified™ Megabase Agarose (Bio-Rad) in 1X TAE buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were next washed and incubated for 1 hour at room temperature with peroxidase conjugated anti-rabbit (1:5000, BioRad), or anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... 18μg of sample was loaded per well diluted 1:1 with freshly prepared 2X Laemmli Sample buffer (BioRad Hercules, CA) + β-Mercaptoethanol (1:20 ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were then treated with HRP-conjugated goat anti-moue secondary antibody (1:10000) diluted in blocking solution for 1 h and developed with enhanced chemiluminescent (ECL) substrate (Cat#1705060, Biorad).
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bioRxiv - Biochemistry 2020Quote: ... the membranes were incubated with goat anti-rabbit antibody conjugated to HRP (1:500 or 1:1000 against MEK or ERK/GST, respectively) (BioRad) for one hour at room temperature ...
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bioRxiv - Pathology 2019Quote: ... After incubation of the blots with HRP-conjugated secondary antibodies (1:3000 for goat anti-rabbit or 1:10,000 for goat anti-mouse, Bio-Rad), specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell and tissue lysates were diluted at a 1 to 1 ratio of lysate to loading buffer and loaded onto Mini-Protean TGX precast gels (BioRad), electrophoresed ...
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bioRxiv - Physiology 2020Quote: ... antibody at 4°C overnight and in goat anti-rabbit IgG horseradish peroxidase conjugated secondary (1:5000) antibody for 1 hour before detection using ECL detection (BioRad) and ChemiDoc Imaging (BioRad) ...