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Citations for Bio-Rad :
301 - 350 of 3318 citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Bovine Interleukin-8 PBP039 and Goat anti Bovine Interleukin-8 Ab conjugated to biotin AHP2817B (all from Bio-Rad, protocol according to the manufacturer’s intructions).
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bioRxiv - Biophysics 2023Quote: The monovalent streptavidin sample was kindly provided by the Howarth Lab (University of Oxford),23 and recombinant IgE Kappa (clone AbD18705_IgE, Cat#HCA190) was purchased from Bio-Rad Laboratories.
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bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Microbiology 2022Quote: ... from 22 people were quantified using a Bio-Plex Pro™ Human Inflammation 24-Plex Panel or a Bio-Plex Pro™ Human Th17 Cytokine Panel 15-Plex Panel (Bio-Rad, Hercules, CA, USA). Cytokines were omitted from further analyses if their concentration was close to the lower limit of detection in most samples ...
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bioRxiv - Biophysics 2020Quote: ... Protein concentrations were determined using unlabeled wild-type proteins (β or PCNA) as standards in a Bradford assay (BioRad). Fluorophorophore concentrations were determined from absorbance measurements and molar absorptivities as described previously (9,10).
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bioRxiv - Biophysics 2019Quote: ... The retention time of both oligomer types was compared with a set of molecular weight protein standards (Bio-Rad) consisting of bovine serum albumin (66.5 kDa) ...
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bioRxiv - Biochemistry 2021Quote: ... Human primers were designed by using Beacon Designer software (Bio-Rad). Quantitative PCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used: anti-human CD53 (MEM-53, Biorad), anti-human CD53-488 (MEM-53 ...
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bioRxiv - Immunology 2020Quote: Human CTL were transfected using a GenePulser Xcell electroporation system (BioRad). 1x106 CTL (5days after restimulation therefore in expansion phase ...
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bioRxiv - Pathology 2023Quote: ... or mouse monoclonal antibodies against human C3d (Bio-Rad Laboratories, CA) and C4d (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... Primers/probes to detect human RPP30 (BioRad Custom Assay, Hercules, CA) were used as an endogenous reference in human samples ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: SDS-PAGE analysis of the recombinant TMPT2A (rTMPT2A) was performed on linear 12.5% polyacrylamide minigels in a Mini-Protean® Tetra cell system (BioRad, USA), as described by [22] ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... and the fractions containing the recombinant vaccine candidates were automatically collected using BioFrac™ Fraction Collector (Bio-Rad Laboratories, Hercules, USA) when the 280 nm absorbance was greater than 0.05 ...
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bioRxiv - Neuroscience 2019Quote: ... capacitance of 3 μF and a resistance of 400 Ω (Gene Pulser II (BioRad)) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were measured using the DC protein assay kit II (BioRad, Cat# 5000112). Laemmli buffer (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR assay was conducted using the SYBR Premix ExTaq II (Bio-Rad) and gene-specific primers (Table S1) ...
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bioRxiv - Biochemistry 2022Quote: ... which were transfected into KNL2 knockout cells with Gene Pulser II electroporator (Bio-Rad). The transfected cells were selected in the medium containing 2 mg/ml G418 (Santa Cruz Biotechnology).
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the DC protein assay kit II (BioRad, Cat# 5000112) and standardized across all samples within an experiment ...
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bioRxiv - Synthetic Biology 2020Quote: ... Electroporation was performed using a Gene Pulser® II (Bio-Rad, Hercules, CA, USA) with a Nepa Electroporation Cuvette with a 1 mm gap (Nepa Gene ...
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bioRxiv - Immunology 2021Quote: ... Gels were imaged using the Bio-Rad Universal Hood Ii Molecular imager (Bio-Rad) and processed by Image Lab Software (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... Protein concentration was estimated using the RC DC protein assay kit II (BioRad, 5000122) with BSA as reference ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined via Bradford assay (Bio-Rad Protein Assay Kit II; (75)) ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein amount was determined by DC™ Protein Assay Kit II (Bio-Rad), then lysates were loaded on 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... the protein concentration was measured using a DC Protein Assay Kit II (Bio-Rad). The cell lysate was denatured at 75 °C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Total protein was determined in all samples by Protein Assay (Protein Assay II, BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined using the DC Protein Assay Kit II (#5000112, Bio-Rad). Proteins (20μg ...
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bioRxiv - Microbiology 2022Quote: ... and 0.25 mM disodium EDTA) by using a CHEF-DR II system (Bio-Rad). CHEF DNA size standard of Saccharomyces cerevisiae (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2023Quote: ... Data were acquired with BD LSR II (Becton-Dickinson) or ZE5 (BioRad, Hercules, CA) flow cytometers ...
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bioRxiv - Biophysics 2023Quote: ... 5 µl of protein samples and standards (Protein Standards I and II, Bio-Rad) were added to 25 µl reagent A’ and mixed in the wells of a 96 well plate (Corning Costar) ...
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bioRxiv - Bioengineering 2023Quote: ... Gels were run in the “Mini Protean II” electrophoresis cell from Bio-Rad (USA) at 200 V until the dye front reached the bottom of the gel.
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bioRxiv - Cell Biology 2023Quote: ... Parasites were electroporated (310 kV, 950 µF) using a Gene Pulser II (Bio-Rad) and transgenic parasites selected for by drug selection using 2.5 µM WR99210 (Jacobus Pharmaceutical Company) ...
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bioRxiv - Physiology 2023Quote: ... supernatant was retained and protein quantified with the DC Protein assay kit II (Biorad). Proteins samples (1.5µg and 3µg of proteins ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was measured using the DC Protein Assay Kit II (5000112, Bio-Rad) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... elegans cDNA was amplified from extracted mRNA from wild type worms using the iScript Select cDNA Synthesis kit (Bio-Rad). KLP-18 cDNA was amplified from whole worm cDNA with gene specific primers and Q5 DNA polymerase ...
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bioRxiv - Molecular Biology 2022Quote: ... and P-TEFb OE and wild-type (w1118) chromatin (CycT) were analyzed by qPCR on a CFX96 Real-Time System (BioRad). qPCR reactions were carried out using 2 μl of ChIP DNA as a template with 300 nM primers and 5X HOT FIREPol® EvaGreen® qPCR Mix Plus (Solis BioDyne ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated by dual Tris-glycine SDS-PAGE (lower quarter 15%, upper ¾ 10% acrylamide gel and transferred to a P-type PVDF membrane using the BioRad wet transfer system ...
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bioRxiv - Immunology 2022Quote: ... Pre-designed human gene primers were purchased from Bio-Rad (supplemental Table). Human PPIA was amplified in parallel and used as the reference gene in quantification ...
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bioRxiv - Biochemistry 2020Quote: ... with goat anti-human IgG F(ab’)2-HRP (STAR126P, Bio-Rad) as the detection antibody.
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bioRxiv - Biophysics 2020Quote: ... and mouse anti-human CD34 (QBEND 10 clone, Ms IgG1, Bio-Rad). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Human primers for HLA-DRB1 and VCAM1 were purchased from Bio-Rad.
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in FACS buffer with Fc-block (Human Seroblock, Bio-Rad). These samples were then re-pelleted and suspended in a cocktail of fluorophore-conjugated primary antibodies (Supp ...
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bioRxiv - Immunology 2020Quote: ... and incubated with either FITC-conjugated Sheep Anti-Human C3 (BioRad AHP031F) at 1:500 or AF488-conjugated Mouse Anti-C5b-9 (ae11 ...