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Citations for Bio-Rad :
301 - 350 of 463 citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Electroporation was performed in 0.1 cm (The Cell Projects) electroporation cuvettes using a Gene Pulser II Electroporator (Bio-Rad) using the following parameters – 12.5 kV/cm ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... an assay detecting the human ribonuclease P/MRP subunit p30 RPP30 gene (Bio-Rad Assay ID dHsaCP2500350) was used as reference (probe ...
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bioRxiv - Cell Biology 2022Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... For the quantification of metabolic hormones Bio-Plex Pro Human Diabetes 10-plex Assay (BioRad, USA; 171A7001M) was used ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated at 370C for a further 24 h with human oxLDL (#5685-3557, Bio-Rad) at a concentration of 0 µg/ml and 25µg/ml ...
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bioRxiv - Cell Biology 2019Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... IL-18 and IL-6 concentrations were measured using the Bio-Plex Pro Human Cytokine Assay (BioRad).
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bioRxiv - Immunology 2022Quote: ... Cytokines and chemokines were measured using the Bio-Plex Pro™ Human Assay (Bio-Rad, Hercules, USA): cytokine screening panel plus ICAM-1 and VCAM-1 (12007283 ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Pathology 2024Quote: ... and cytokines were detected using the Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, #M5000031YV), including IL-1β ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:4 and analyzed using Bio-Plex ProTM Human Chemokine Panel 40-plex Assay (Bio-rad). Acquisitions and analyses were performed on a Bio-Plex 200 system with Manager 6.1 Software (Bio-rad) ...
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bioRxiv - Immunology 2024Quote: ... Approximately 600 RUs of anti-human IgG (Cytiva) were immobilized on a GLC Sensor Chip (Bio-Rad) using amine-coupling ...
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bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... Electroporation was performed at 0.31 kV and 950 μF with maximal capacitance using a Gene Pulser II system (Bio-Rad). The parasites were then cultured in complete medium at 1% hematocrit at 37 °C ...
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bioRxiv - Immunology 2020Quote: The VSGm protein samples were separated on 10% SDS-PAGE then trans-blotted onto two separate nitrocellulose membranes using the mini PROTEAN II Trans-blot unit (Biorad). The membranes were rinsed in 1x PBS (0.1% w/v ...
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bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to myosin II was estimated by a gel-shift assay (4–15% gradient gel, Biorad) and determined as >99% for both S1 and lever-arm-less S1 (Supplementary Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosomal DNA was separated in 1% agarose/0.5 × TBE on the CHEF-DR II Pulsed Field Electrophoresis Systems (Bio-Rad) as follows ...
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bioRxiv - Molecular Biology 2020Quote: Yeast genomic DNA was prepared in an agarose plug as described in the Instruction Manual (Bio-Rad, CHEF-DR II) with slight modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasma vitamin B12 concentrations were assessed using the BioRad Quantaphase II radio assay (Hercules, CA, USA;74. Plasma folate concentrations were also measured using the BioRad Quantaphase II radio assay (Hercules ...
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bioRxiv - Plant Biology 2023Quote: ... A quantitative real-time PCR (RT-qPCR) assay was performed on a LightCycler 480 II system using 2x SYBR Green qPCR master mix (BioRad). Maize GAPDH (Zm00001eb233140 ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-FLAG-HRP (clone D6W5B, CellSignalingTechnology #86861S, RRID:AB_2800094) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P, RRID:AB_609796) detection Abs were added for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Linearized plasmid was mixed with 250 μL of cells at 15°C in a 0.4 cm gap electroporation cuvette and transformed immediately by electroporation using a Gene Pulser II (Bio-Rad) set to 800V and 25 μF ...
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bioRxiv - Microbiology 2020Quote: ... Analyses were performed with the Extended Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Biorad, Munich, Germany) as described previously 72 and using a 2-laser reader allowing simultaneous quantification of cytokines and chemokines ...
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bioRxiv - Cancer Biology 2019Quote: ... Collected sera were subjected to ELISA by capturing with human anti-rituximab idiotype antibody (HCA186, Bio-Rad Laboratories), detected with rat-anti-rituximab-HRP antibody (MCA2260P ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated EVs were stained with PE-labelled anti-human TNFR1 (Bio-Rad, cat #MCA1340PE, clone H398, lot 0407) and APC-labelled anti-mouse gp130 antibodies (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... We used mouse anti-human HLA-ABC (W6/32) conjugated to Alexa Fluor® 647 (MCA81A647, Bio-Rad), which is known to also react with MHC-I of African Green monkey from which Vero E6 cells are derived(48 ...
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bioRxiv - Immunology 2019Quote: ... fatty acid free BSA Efflux was measured after a 4-hour incubation period with 0µg/ml and 50µg/ml of human HDL (#5685-2004, BioRad). Supernatants were collected and the cells lysed with 1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Zoology 2019Quote: ... Equal amounts of total protein per sample were separated by SDS gel electrophoresis on a 12.5% polyacrylamide gel [39] using a Mini-PROTEAN II Electrophoretic Cell (Bio-Rad, USA). Wet-transfer of the proteins from the gel to a polyvinylidene difluoride transfer membrane (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: Suprarenal aortas /AAA were isolated from Apoe−/−Il27ra+/− and Apoe−/−Il27ra−/− mice implanted with Angiotensin II pumps and homogenized in PureZol (Bio-Rad) with Ceramic Beads using Omni Bead Ruptor 24 ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
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bioRxiv - Microbiology 2020Quote: ... We covered the wells with a coverslip and we let them polymerize in a UV transilluminator (Bio-Rad Universal Hood II) for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The mixture was subjected to two consecutive electrical pulses of 850 V at 20 μF with a Gene Pulser II electroporator (Bio-Rad) and the cells were then seeded on a confluent monolayer of LR7 feeder cells in a 35mm dish ...
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bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
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bioRxiv - Developmental Biology 2021Quote: ... and 25 µg of the targeting vector was transfected into 1 × 107 of Nmt1-heterozygous cells11 by electroporation (240 V, 500 µF) with Gene Pulser II (Bio-Rad). One week later ...
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bioRxiv - Microbiology 2020Quote: ... baumannii cells using a Bio-Rad GenePulser II (1.8 kV, 25 μF, and 200 Ω) in a 1 mm electrode gap cuvette (Bio-Rad). Cells were recovered by resuspension in 1 mL of SOC medium and incubated at 37°C with shaking (200 rpm ...
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bioRxiv - Zoology 2020Quote: ... Telomeric repeats of sequence TTAGG were separated from restricted DNA with Pulsed Field Gel Electrophoresis (CHEF-DR II system by Bio-Rad) in a 0.8% agarose gel.
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bioRxiv - Molecular Biology 2019Quote: ... and an ATG initiation codon was co-electroporated with the pCAGGS-FLPe-puro into KH2 ESCs at 500V and 25 µF using a Gene Pulser II (Bio-Rad). Cells were selected with hygromycin (140 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with cold water and 10% glycerol and electroporated with the cat or kan PCR product using a GenePulser II instrument (2.5 kV, 0.2 cm gap cuvettes; Bio-Rad, Hercules, CA). Transformants were verified by colony PCR with primers approximately 200 bp up- and downstream of the gene of interest ...
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bioRxiv - Microbiology 2020Quote: ... Samples were assayed by Taqman multiplex real-time PCR using TaqMan Universal Master Mix II and a CFX384 Touch™ Real Time PCR detection system (BioRad). A standard curve was obtained using an in vitro transcript derived from a synthetic plasmid that contained all PCR targets ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reactions were analysed by agarose gel electrophoresis and image capture was performed on a Universal Hood II Gel Doc System (Bio-Rad).