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Citations for Bio-Rad :
251 - 300 of 463 citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... mouse anti Human Protein Gene Product 9.5 (PGP9.5) (1:500, MCA4750GA mouse monoclonal, Biorad) for 36 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... for detecting mLIF or Bio-Plex Pro Human Cytokine LIF Set (Bio-Rad, 171B6011M) for detecting hLIF ...
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bioRxiv - Microbiology 2020Quote: ... incubated for five minutes in dark and visualized using Universal Hood II Gel Doc System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... and cluster analysis was carried out using Molecular Analyst software – Fingerprinting II (Version 3.0, Bio-Rad, CA). The similarity matrix was calculated on the basis of the Dice coefficient ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was determined using a commercial colourimetric assay (Bio-Rad Protein Assay kit II, Bio-Rad Laboratories Pty Ltd ...
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bioRxiv - Cell Biology 2021Quote: ... was amplified by electroporation using a Bio-Rad Gene Pulser II electroporation apparatus (Bio-Rad #165-2105) and the Lucigen Endura bacteria (Lucigen ref ...
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bioRxiv - Cancer Biology 2019Quote: ... and transfection was performed by electroporation using a Gene Pulser II electroporator (BIO-RAD, Hercules, CA, USA) at 210 V and 975 µF for Western Blot analyses ...
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bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad) using the following oligonucleotides ...
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bioRxiv - Biochemistry 2020Quote: Protein concentrations were determined using the BioRad DC Protein Assay kit II (BioRad, Marnes-la Coquette, France) in accordance with manufacturer’s instructions and using bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM EGTA) and protein concentrations were determined by DC protein assay kit II (Bio-Rad #5000112). Proteins were separated on house-made SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham #10600003) ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were separate in 10% SDS-polyacrylamide gel using the Miniprotean II electrophoresis system (Bio-Rad). The gel was stained with a fixative solution of 40% MeOH ...
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bioRxiv - Plant Biology 2022Quote: ... Concentration of nuclear proteins was determined using the Bradford assay (Protein Assay Kit II, Bio-Rad, USA). Nuclear protein extract (20 µg ...
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bioRxiv - Neuroscience 2022Quote: ... Protein quantification was done using a BSA standard following the DC Protein Assay Kit II (Bio-Rad) and read on microplate reader ...
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bioRxiv - Molecular Biology 2019Quote: ... Measurement of serum cytokines was performed using multiplex ELISA (human 27-plex #m500kcaf0y, Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked by using PBS with 5% human AB serum (Bio-Rad, Germany) and 5% mouse serum (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... and human 18S genes using the iQ™ SYBER® Green Supermix (Bio-Rad, #1708880) with the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... For cytokine quantification Bio-Plex Pro Human Cytokine Screening Panel 48-plex (BioRad, USA; 12007283), Bio-Plex Pro Human Cytokine ICAM-1 (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... and the Bio-Plex Pro™ Human Inflammation Panel-1 Kit (37-Plex, Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... First-strand complementary DNA was synthesized from isolated RNA using iScript II reverse transcription supermix (1708841; Bio-Rad). Prepared cDNA was mixed with SsoAdvanced Universal SYBR Green Mix (172570 ...
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bioRxiv - Molecular Biology 2020Quote: ... IPG strips were run upon SDS PAGE (12.5% acrylamide) using the Protean® II 2-D Cell (BioRad). Gels were then Coomassie blue stained (Phastgel Blue R ...
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bioRxiv - Pathology 2019Quote: ... 1.5mm) using the Biorad variant II capillary collection device (Bio-Rad catalog number 196-2050, BioRad 510(k) K142448) ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were visualized either using film exposure or the Universal Hood II Gel Molecular Imaging System (Bio-Rad). Each experiment was repeated at least three times and produced similar results.
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bioRxiv - Physiology 2021Quote: ... Quantitative PCR was performed with a Roche LightCycler 480 II using SYBR green supermix reagents (Bio-Rad Laboratories). A panel of reference genes was utilized to determine the most stably expressed reference target (NormFinder ...
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bioRxiv - Neuroscience 2020Quote: ... and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories). Protein was transferred to a nitrocellulose membrane (Hybond ECL ...
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bioRxiv - Microbiology 2022Quote: ... The purified 3-cycle PCR products were qPCR quantified using Platinum SuperFi II on a CFX96 system (BioRad). The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat ...
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentrations of the lysates were quantitated using a DC Protein Assay II Kit (BioRad, 5000112.). Protein A Dynabeads (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... After obtaining the brain and Neuro2A lysates we measure protein concentration (DC™ Protein Assay Kit II, Biorad) and samples were used in subsequent experiments.
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bioRxiv - Genomics 2023Quote: Pulsed-field gel electrophoresis (PFGE) was performed using a BioRad CHEF II apparatus (BioRad Laboratories, Hercules CA, USA). Chromosomal plugs were placed in the wells of a 1.2% (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was transferred and protein concentration was determined using DC Protein Assay Kit II (BioRad, Hercules, CA). The pellet was resuspended in 100 μl of lysis buffer including DNase (10 U/ml ...
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bioRxiv - Systems Biology 2024Quote: ... protein quantitation was performed using the Bio-Rad Protein assay kit II (Bio-Rad Laboratories, Hercules, United State) and peptide quantitation was performed using the Pierce™ Quantitative Peptide Assays & Standards (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... total protein concentration was measured using a protein assay (DC™ Protein Assay Kit II, BIO-RAD, 5000112). Activin A measurements were normalized to total protein levels ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and electroporated at 280 V and 725 μF using a Gene Pulser II (Bio-Rad Laboratories). After electroporation ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (1 μg) was used to generate the ProtoScript II First Strand cDNA Synthesis Kit (Biorad E6560S) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed using Superscript II RT and random hexamer primers according to the manufacturer’s instructions (BioRad). qRT-PCR was performed in triplicate with the BioRad C1000 Touch™ Thermal Cycler and CFX384™ Real-Time System using SYBR Green PCR Master Mix (BioRad ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with mouse anti-human secondary antibody conjugated to HRP (1721050, Bio-Rad Laboratories). HRP binding was determined based on reaction with TMB substrate (34028 ...
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bioRxiv - Immunology 2020Quote: Supernatants were thawed and immediately analyzed with Bio-plex human cytokine assays (Bio-Rad Laboratories, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into K562s using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Immunology 2024Quote: ... 17 human cytokines were analysed simultaneously using the kit and run on the Luminex analyser (BIORAD), standard curves for each cytokine created and per cytokine concentrations calculated using the BioRad software.
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Protein concentrations were determined by use of the Bradford assay (Bio-Rad protein assay kit II, Cat. No. 5000002). For immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μg of in vitro-transcribed RNA (2.5 μg of SNX27/LRFN2 RNA and 2.5 μg of the defective helper plasmid) was electroporated into 0.6×107 BHK-21 cells using a Gene Pulser II electroporation system (BioRad) in a gene pulser cuvette (0.2 cm gap) ...
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bioRxiv - Biochemistry 2021Quote: ... Transcriptionally active Q0.3 fraction (0.32–0.4 M) were pooled and applied directly to hydroxyapatite (HAP) type II ceramic resin (Bio-Rad), washed first at 0.38 M ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were then separated on 12 % polyacrylamide gels (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France) and transferred to 0.2 μm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The virus genome solutions of each well were homogeneized by shaking and two 2 μL aliquots were taken for qPCR titration using the my-Budget 5x EvaGreen qPCR-Mix II (Bio-Budget) following the manufacturer’s instructions and a CFX96 Real-Time System machine (BioRad). See supplementary table 1 for primers.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Supernatant was recover and protein concentration was measured using the DC™ Protein Assay Kit II (Bio-Rad, 5000112). Lysates were boiled in the NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Physiology 2024Quote: ... The total protein content was determined using the colorimetric Bio-Rad Protein Assay Kit II (Bio-Rad Laboratories, CA). Seven dilutions of a protein standard containing 0 to 30 µg/mL of the total protein content were used ...
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bioRxiv - Microbiology 2023Quote: ... 30 μg DNA total) by electroporation with a time constant between 0.16 and 0.20 msec (BioRad Gene Pulser II). After 24 h ...
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bioRxiv - Genomics 2023Quote: ... to ensure high integrity and later analyzed with pulse-field gel electrophoresis on CHEF DR-II (BioRad, CA, USA) to ensure the size range between 20 kb to 100 kb ...