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Citations for Bio-Rad :
301 - 347 of 347 citations for Fmoc Rink Amide OctaGel Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer C + 400 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Glycosylated products were separated from unincorporated UDP-[14C]-GalNAc by anion exchange chromatography using AG1-X8 resin columns (Bio-Rad #1401454), and product incorporation was determined by liquid scintillation counting (Beckman Coulter LS6500) ...
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bioRxiv - Biochemistry 2021Quote: Primary screening for nanobody binding was achieved by a small-scale pulldown method in which Affi-gel 10 affinity resin (Bio-Rad) was bound to laforin and subsequently to each purified VHH clone34 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was subjected to affinity chromatography at 4°C composed of 75 μL of amylose resin bound to 200 pmol of MS2-MBP protein in a Bio- Spin disposable chromatography columns (Bio-Rad). After washing ...
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bioRxiv - Molecular Biology 2022Quote: ... at a 1:5 w/w ratio for 2 hours before addition of 100 mg mL-1 Bio-Beads SM-2 resin (Bio-Rad) and then incubated overnight at 4 °C with gentle agitation to remove detergent ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2021Quote: ... according to the instructions of the manufacturer or extracted using Chelex® 100 resin (Bio-Rad Laboratories Inc., Hercules, CA USA). Two final elutions of DNA were made from the kit extractions with the first being used for construction of genomic libraries for a related study and the second being used for screening of Vssc mutations after being diluted 1:5 with water ...
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bioRxiv - Biophysics 2020Quote: ... The buffers used in purification protocol were prepared using deionized water which was passed through a chelex resin column (Bio-rad) to exclude metal contaminants and obtain metal-free eluate ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blot analysis was accomplished through SDS-PAGE of cell lysates or affinity-resin eluates in 1X laemmli loading-buffer (Bio-Rad) containing β-mercaptoethanol (1/10 in 4X buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated conditioned medium was fractionated by size exclusion chromatography (SEC) by application to Sepharose CL-2B resin (17014001, Cytiva) stacked in a disposable Econo-Pac column (7321010, Bio-Rad). The column was eluted with PBS and 0.5 ml fractions were collected ...
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bioRxiv - Plant Biology 2024Quote: Each clarified lysate was incubated with 500 µL bed volume of ProfinityTM IMAC Ni-Charged Resin (156-0135, Bio-Rad, USA) in Poly-Prep® Chromatography Columns (731-1550 ...
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bioRxiv - Immunology 2022Quote: ... 10 mM sodium bicarbonate in iron-free HEPES-buffered saline (HEPES 20 mM, NaCl 150 mM, treated with Chelex-100 chelating resin [Bio-Rad, Hercules ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant proteins lacking the 6His tag were further purified via reverse IMAC using 0.8 ml Ni2+- loaded Chelating Sepharose Fast Flow resin packed into poly-prep® chromatography columns (cat. Number 7311550 – Bio-Rad) and prepared as above ...
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bioRxiv - Biophysics 2023Quote: ... The myosin in solution was separated from the stripped-off resin using a Micro Bio-Spin™ Chromatography Column (Bio-Rad) and loaded on a 1 mL HiTrap Q HP column (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos surviving for 24 h post- injection were incubated in 100 µl of 5 % Chelex® 100 Molecular Biology Grade Resin (BioRad) and 50 µl of Proteinase K Solution (20 mg/ml ...
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bioRxiv - Immunology 2024Quote: The pellet can be saved as a source of LPS and the soluble protein antigens were treated overnight with Affi-Prep Polymyxin Resin (Bio-Rad) to remove trace amounts of LPS remaining.
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bioRxiv - Biochemistry 2024Quote: ... The resin bound to the recombinant sdAb was then packed in a Poly-Prep chromatography column (Bio-Rad, Hercules, CA, USA) and washed with 10 column volumes of TBS followed by washing with 10 column volumes each of TBS containing 10 mM and 20 mM imidazole ...
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bioRxiv - Genetics 2024Quote: ... crosses with gRNA-expressing males (GuideA.1-2; GuideB.1-2) was extracted using an adapted Chelex 100 Resin (Bio-Rad) protocol ...
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bioRxiv - Microbiology 2022Quote: ... Milli-Q water was treated with Chelex 100 resin 100–200 mesh sodium form via batch method (50 g/L, Bio-Rad Laboratories). A concentrated stock of SD media not containing Cu2+ ...
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bioRxiv - Microbiology 2020Quote: ... containing 2 g l-1 glucose and 1.32 mM K2HPO4 by treating prepared media with a universal chelator (Chelex® 100 Chelating Resin, Bio-Rad 1421253), filtering sterilizing away the Chelex resin ...
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bioRxiv - Microbiology 2023Quote: ... the mixes were incubated for a minimum of 15 min at 4 °C before passing them (0.5 - 1.0 mL/min) through StrepTactinXT Superflow (IBA GmbH, Göttingen, Germany) resin beds packed in Econo-Column chromatography columns (Bio-Rad, CA, USA). Columns were washed with ten column volumes of 1x Buffer W and elution of the retained proteins was performed with 1x BXT elution buffer (IBA GmbH ...
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bioRxiv - Physiology 2024Quote: ... T3 was extracted from supernatants and purified through solid-phase chromatography using 200 – 400 anion exchange chloride resin (Bio-Rad, 140-1251) in Poly-Prep chromatography columns (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... sodium cations were exchanged with pyridinium ions by dissolving 20 mg of fucan in water and passing the solution through an AG® 50W cation exchange resin (Bio-Rad), pre-equilibrated with pyridine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... fractions with Afl RT were pooled and loaded onto a 2-ml Macro-Prep DEAE Resin (Bio-Rad, column CA, Hercules, USA) pre-equilibrated with a buffer C (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were added to a 2 mL bed of Ni-NTA agarose resin in a 20 mL column (Bio-Rad 7321010) equilibrated with wash buffer (50 mM Tris-HCl (pH 8) ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... concentrated fractions containing CDT holotoxin were loaded onto a gravity flow size exclusion column (1 g, P-60 resin; #1504164, Bio-Rad, USA) pre-equilibrated with PBS pH 7.4 as the mobile phase and 500 μL elution fractions taken.
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bioRxiv - Genomics 2024Quote: ... The TALON® resin-lysate mixture was then passed through a gravity flow Poly- Prep Chromatography Column (Bio-Rad Laboratories, Hercules, CA). Non-specifically bound proteins were removed by washing the TALON® resin with 10 mL of Lysis Buffer ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges, BIO-RAD 7324661), then dialyzed ...
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bioRxiv - Biophysics 2022Quote: ... the samples were cooled on ice for 10 min followed by the addition of 80 mg Bio-beads SM-2 Resin (Bio-Rad, Hercules, CA), then rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... His-tagged protein was bound to 2mL HisPur™ Ni-NTA Resin (Thermo 88221) equilibrated with lysis buffer in a glass Econo-Column® Chromatography Columns (Bio-Rad) by flowing through the clarified lysate ...
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bioRxiv - Immunology 2024Quote: ... the fractions were eluted with 0.25 M NaCl and biologically active fractions were further analyzed on a hydrophobic interaction column (Macro-Prep® t-Butyl HIC Resin, Bio-Rad 1580090). Three fractions eluted with buffers with pH=7 ...
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bioRxiv - Biophysics 2023Quote: ... The ‘free’ dye was separated from the labeled protein by binding the reaction mixture to a 250 μL cobalt affinity resin column equilibrated with 15 CV CoWB (no TCEP) in a Micro-Bio spin chromatography column (Bio-Rad Laboratories # 7326204), washed 15 CV with CoWB and then eluted with 400 mM imidazole in CoWB ...
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bioRxiv - Plant Biology 2023Quote: ... Native protein extracts from E.coli or Synechococcus ΔCS were incubated in IMAC HEPES binding buffer supplemented with potential effector molecules and Ni-charged iminodiacetic acid Profinity™ IMAC Resin (Bio-Rad, USA) on a low speed rotator for 1 h at 23°C ...
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bioRxiv - Biochemistry 2023Quote: ... The neutralized sample was then loaded onto a packed ion exchange resin (DOWEX H+) in a poly-prep chromatography column (Bio-Rad, 731-1550). The column was then washed with 5% acetic acid ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged protein was bound to 3 mL HisPur™ Ni-NTA Resin (Thermo 88221) equilibrated with lysis buffer in a glass Econo-Column® (Bio-Rad) by flowing through the clarified lysate ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodiscs were assembled by mixing MSPΔH5 with solubilized lipids at a ratio of 1:45 (final cholate concentration of 18 mM) followed by the removal of cholate from the mixture with Bio-Beads SM2 resin (Bio-Rad, 152-8920), under o/n rocking at 22° ...
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bioRxiv - Genetics 2024Quote: ... which were dried on tissue paper before homogenizing whole individuals in 100 µL of 5% Chelex-100 resin (Bio-Rad, Hercules, CA, USA) in ultrapure water with 2.5 µL proteinase K (20 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...
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bioRxiv - Biochemistry 2023Quote: ... ACN was added to a final concentration of 75% and the sample loaded onto Bio-Gel P10 Gel resin (Bio-Rad, Hercules, CA, USA) previously conditioned with 80% ACN ...
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bioRxiv - Systems Biology 2023Quote: ... 1,000 µL lysate aqueous phase was added to 300 µL 37.5% (w/v) AG 1-X8 anion exchange resin (Bio-Rad, Hercules, CA, USA: 1401441) and washed with 3 mL water to remove p2DG ...
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bioRxiv - Microbiology 2020Quote: The pure cultures were grown in TSAYE plates for 24 h and DNA was extracted using 2% Chelex solution (Chelex 100 resin, Bio-Rad laboratory, Hercules, California, USA). The colonies (2-3 mm ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from the isolates using the InstaGeneTM Matrix comprising 6% (w/v) Chelex resin for PCR-ready DNA purification (Bio-Rad #7326030; California, United States). DNA quality was checked by measuring the A260/280 ratio using NanoDrop ...
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bioRxiv - Biochemistry 2023Quote: ... The resin was recovered and washed twice with 10 column volumes of S100 Lysis Buffer on a gravity flow column (Bio-Rad Laboratories Inc., Hercules, CA). The S100 extract was eluted from DEAE resin with 5 ml of S100 Elution Buffer (250 mM KHPO4 (pH 6.5),1x Mini protease inhibitor cocktail tablet (Roche ...
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bioRxiv - Biochemistry 2021Quote: The proteins extracted from Ni-NTA resin were subjected to SDS-PAGE on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA, USA) using the standard procedure ...