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Citations for Bio-Rad :
3351 - 3400 of 4293 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 Integrase 2E3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HRP detection was performed by adding 1 mL Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal total protein concentration were heated at 90°C in 1 × loading buffer (Bio-Rad 1610747) for ten minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: Western Blot samples of unbound fractions and IP were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Neuroscience 2022Quote: ... horseradish peroxidase (HRP) conjugated secondary antibodies (1: 5000) and signal detected using ClarityTM Western ECL Substrate solution (BioRad). Blots were visualised on a ChermiDOC XRS+ system (BioRad ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (Biorad ...
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bioRxiv - Biochemistry 2019Quote: Complementary DNA was synthesized from 1 μg of purified RNA using the iScript cDNA Synthesis Kit (BioRad, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Both PK-treated and untreated samples were then mixed 1:2 with 4X Laemmli sample buffer (Bio-Rad) containing DTT ...
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bioRxiv - Microbiology 2019Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF) and protein concentration was determined using the DC protein assay (Bio-Rad). Whole cell lysates were resolved by SDS-PAGE in 4-20% gradient Mini-PROTEAN TGX gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at RT and detected using Clarity or Clarity Max Western ECL Blotting Substrate (Bio-Rad) and imaged (Amersham AI600) ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were incubated for 1 h on ice and finally measured using the S3e™ Cell Sorter (BioRad) apparatus ...
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bioRxiv - Evolutionary Biology 2020Quote: ... NCBI accession DQ278441.1) (Table 1) were used for quantitative PCR using the iQ SYBR Green Supermix (BioRad, Canada) and the following cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 1 mg of RNA using the iScript cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 h at rtp with 5% (w/v) non-fat milk block (Bio-Rad) in 1x TBS (in mM ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 30 min at room temperature with α-CD3 (1 µg/mL; clone UCHT1; Bio-Rad). CD8+ T cells were negatively selected from blood and TDL using a CD8+ T Cell Enrichment Kit (stemCell Technologies) ...
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bioRxiv - Immunology 2019Quote: ... purified PR3 mutant proteins were loaded (1 µg/lane) onto 12% Tris-HCl gels from BioRad (Hercules, CA). The SDS gel electrophoresis was performed at 180 volts for 35 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... then 3 washes in 1% milk were performed prior to imaging the blot with ECL (BioRad Clarity #1705060). Membrane was stripped using the GM Biosciences One Minute Advance Stripping buffer #GM6031 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription of 1 μg of RNA was carried out with iScript cDNA Synthesis Kit (1708891, Bio-Rad) using an extended transcription step of three hours at 42°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4970) overnight at 4 °C and the HRP-conjugated secondary antibodies (BIO-RAD, Cat. No. 1706515, 1:1000) for 1 h at room temperature ...
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bioRxiv - Physiology 2020Quote: ... and reverse transcribed into cDNA using 1 μg of RNA and iScript Reverse Transcriptase (Bio-Rad, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked 1 hour in skim milk 5% TBS-Tween buffer (TBST, Bio-Rad, 0.05% Tween 20), and incubated overnight with the primary antibodies diluted in blocking buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10% (19:1) PAGE was prerun for 30 minutes at 100V in a Mini PROTEAN tank (Bio-Rad) electrophoresis chamber using 1X TBE (89 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM DTT) before being loaded on a HiTrapQ anion exchange column using the NGC FPLC (Bio-rad). PfQC was eluted using a salt gradient ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 ug of total RNA was converted into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad). RT-qPCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Hydrogels of varying stiffness were prepared using varying ratios of 40% acrylamide to 1% bis-acrylamide (Bio-Rad) in a mixed solution with water ...
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bioRxiv - Neuroscience 2020Quote: ... Three replicas of 1.5μl of a 1:3 dilution of cDNA were amplified using SsoFast EvaGreen Supermix (BioRad) for FACS-sorted microglia and Power SYBR Green (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 25 µg (1 µg/µL concentration) of plasmid DNA was added to electroporation 4 mm cuvettes (Bio-Rad) first and then cells added to the cuvettes ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA (1 ug RNA per reaction) using the iScript cDNA Synthesis Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix and amplification was run on a CFX96 thermal cycler (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized using a 1-μg aliquot of RNA and the iScript cDNA Synthesis system (Bio-Rad). qPCR was performed using human-specific primers (Table S1 ...
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bioRxiv - Immunology 2022Quote: ... and complementary DNA was reverse transcribed from 1 μg total RNA using iScript cDNA Synthesis Kit (Bio-Rad). mRNA expression was measured using iQ SYBR Green on a CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... OrgangeG gel loading dye was mixed with PCR product and ran in a 1% agarose gel (Bio-Rad Certified Molecular Biology Agarose 1613102 ...
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bioRxiv - Genomics 2022Quote: ... using 0.5 × Tris-Borate-EDTA buffer and a 1% gel with low melting point agarose (Bio-Rad #1620137). Gels were stained with 5 μg/ml ethidium bromide in water after electrophoresis for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... This was followed by incubation with HRP conjugated secondary antibody (1:10000) and developed with ECL solution (BioRad).
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bioRxiv - Physiology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, CA, USA). In Quantitative real-time PCR (qPCR) ...
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bioRxiv - Microbiology 2022Quote: ... and stat1a- or stot1b-specific primers (Table 1) in a CFX96 touch RT-PCR detection system (Bio-Rad) using Hardshell® Low Profile Thin-wall 96-well skirted PCR plates (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 µL brain lysate (1ug/uL stock determined by BCA (5000001, Bio-Rad Protein Assay Kit I). After 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Neuroscience 2022Quote: ... Following measurement of the protein concentration using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Plant Biology 2024Quote: ... Purified RNA (1 μg) was used for reverse transcription using the iScript™ cDNA Synthesis Kit (BIO-RAD). RT-qPCR was performed using SYBR green (ThermoScientific ...
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bioRxiv - Microbiology 2024Quote: ... Screens were analyzed using a Molecular Imager FX and the Quantity One 1-D Analysis Software (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... India (Table 1) on a CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad). The Bio-Rad CFX Maestro Software ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on 12% SDS-polyacrylamide (29:1) or 4-15% precast polyacrylamide mini-gels (Bio-Rad) and electrotransferred on nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-PCR was performed on 1:10 cDNA dilutions using Sso Fast EvaGreen supermix (Bio-Rad, 172-5280) on a thermocycler (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a 1 ml HisTrap (Cytiva) column connected to NGC Quest 10 (Bio-Rad), previously equilibrated with Buffer A (25 mM Tris–HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (1 μg) was used to generate the ProtoScript II First Strand cDNA Synthesis Kit (Biorad E6560S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... 20 mM imidazole and 1 mM DTT) and were then transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gels were run for 1 hour 15 minutes at 150V in Criterion vertical cell electrophoresis device (Bio-Rad) and stained for 10-15 minutes using SYBR Gold (ThermoFisher) ...