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Citations for Bio-Rad :
3601 - 3650 of 4293 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 Integrase 2E3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The labeled HA disaccharides were electrophoresed in a Bio-Rad mini-PROTEAN Tetra system using a gel composition of 20% acrylamide (37.5:1; Bio-Rad), 40 mM Tris acetate (pH 7.0) ...
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bioRxiv - Immunology 2021Quote: ... serially diluted rabbit serum (5-fold dilution starting with 1:80) were tested for binding to color-coded beads by Bio-plex (Biorad). The beads were coated with avi tagged C.1086 WT ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000) followed by a chemiluminescence reaction (Clarity Western ECL Substrate; Bio-rad). For the SS-TOM lines analysis ...
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bioRxiv - Neuroscience 2020Quote: ... RNA (1 μg) was reverse-transcribed to cDNA with the iScript ™ cDNA Synthesis Kit (Bio-Rad Laboratories, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Gel was stained for 1 h with 0.1 μg/ml ethidium bromide and visualized by ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and eIF2B4 loci were amplified with the primer pairs detailed in Table 4 and run on a 1% agarose gel and imaged using a ChemiDoc XRS+ imaging system (Biorad). The expected WT fragment length for the eIF2B1 ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was diluted 1:100 and real-time PCR was performed in triplicate with iQ SYBR Green Supermix from BioRad.
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was synthesized from 1 μg of isolated RNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05 ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was let dry and thereafter blocked on a shaker for 10 min in 15 ml blocking buffer (1% casein in TBS, BioRad). The blocking solution was discarded ...
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bioRxiv - Biophysics 2020Quote: ... samples were washed for two minutes with degassed polyacrylamide (PA) solution consisting of 4% (vol/vol) 19:1 acrylamide/bis-acrylamide (161010144, BioRad), 60 mM Tris-HCl pH 8 (AM9856 ...
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bioRxiv - Neuroscience 2020Quote: ... We used 1 microgram of the total RNA for cDNA synthesis (iScript cDNA Synthesis Kit, Cat. #170-8891, Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was treated with ECL (Immobilon Western chemiluminescence HRP substrate) for 1 min and visualized with the ChemiDoc MP imaging system (BioRad). Exposure was optimized for each blot.
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bioRxiv - Microbiology 2020Quote: ... Visualisation and quantification were carried out using a Personal Molecular Imager with Quantity One 1-D analysis software (Bio-Rad) and data were analyzed with GraphPad Prism 4 software.
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bioRxiv - Plant Biology 2020Quote: ... and cDNA was synthesized from 1 µg DNA-free RNA using the iSCRIPT cDNA synthesis kit (BioRad, Hercules, CA, U.S.A). Real-time PCR master mixes were prepared using iQ sybr Green Mix (BioRad ...
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bioRxiv - Systems Biology 2020Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μl of ChIP-ed or input (diluted 30-fold to bring it to 1%) DNA was used in 20 μl total volume reaction using SYBR green master mix (BioRad) and 0.4 μM of each primer ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... Gel plugs were loaded onto 1% agarose gels and electrophoresed in the CHEF Mapper XA system (Bio-Rad, Hercules, CA) at 14℃ ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of total RNA was reverse transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Feldkirchen, Germany). Real-time PCR was performed using Platinum SYBR Green qPCR Super Mix-UDG (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The Taqman probes (Suppl Table 1) used were the following: droplets were generated with a QX100 droplet generator (Bio-Rad), after mixing 20 µl of reaction mix and 70 µl of droplet generator oil (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Cancer Biology 2020Quote: The isolated total RNA (1 μg) was reverse-transcribed to produce cDNA using iScript Reverse Transcription Supermix kit (Bio-Rad). Real-time PCR was performed using SYBR Green supermix (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Microbiology 2022Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 h at 4 °C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on native acrylamide gels containing 0.5X TBE buffer and 7% acrylamide (made from a stock of 40% acrylamide with a ratio of 29:1 acrylamide:bisacrylamide; Bio-Rad). The running buffer for the gels was 1XTBE ...
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bioRxiv - Cancer Biology 2022Quote: ... and the pellet was resuspended in 1 ml of BM1* and cells were counted using a TC20 Automated Cell Counter (BioRad). Single-cell suspensions were mostly re-seeded at the same density as their parental/previous generation/passage.
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The protein sample was incubated at 40°C and 80 °C respectively for 1 h in a PCR cycler (BioRad) with a heated lid to prevent evaporation ...
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bioRxiv - Bioengineering 2022Quote: ... Tricine-SDS-PAGE experiments were performed using a single layer 0.75 mm thick 20% acrylamide/bis-acrylamide gel (using 29:1 Acrylamide/Bisacrylamide, Bio-Rad) run at 120 V constant voltage (Pharmacia LKB ...
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bioRxiv - Cancer Biology 2022Quote: ... Up to 1 µg of total RNA was reverse transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) or gDNA Clear iScript cDNA Synthesis Kit for patient-derived tumor cells (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
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bioRxiv - Biochemistry 2021Quote: ... The same samples were also detected by HRP conjugated StrepTactin antibody at 1:10000 dilution (Bio-Rad catalog number 161038). To allow detection of endogenous selenok ...
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bioRxiv - Molecular Biology 2019Quote: ... The agarose plugs were then loaded onto a 1% agarose gel and electrophoresed in 0.5% Tris-borate-EDTA in a pulse-field apparatus (Bio-Rad) at 14 °C (6 V/cm ...
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bioRxiv - Genomics 2020Quote: ... Membranes were washed using tris-based saline buffer solutions with 0.1% tween-20 (TBST) and blocking solutions were prepared using 5% w/v western-blot grade dry milk powder (Biorad). Membranes were blocked with blocking buffer for 1 hour and washed between incubations for 5-10 mins (3x) ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... DNA product was diluted 1:10 and used to set up quantitative PCR reactions using Sybr Green (Biorad #170-8880). Primers were obtained from ID Technologies:
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bioRxiv - Molecular Biology 2020Quote: ... 1 µg of RNA from each sample was reverse-transcribed in 40 µl using iScript™ cDNA Synthesis Kit (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... in TBS-T (TBS supplemented with 0.1% (v/v) Tween-20) and 1% (w/v) Blotting-Grade Blocker (Bio-Rad) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μL of electrocompetent cells were transformed with 1 ng of the lesion-containing plasmid mixed with 1 ng of pVP146 (internal control for transformation efficiency) by electroporation using a GenePulser Xcell (BioRad) at 2.5 kV ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed 3 times for 5 minutes with TBS-T prior to incubation in ECL reagent for 1 minute and imaging using a Chemidoc MP (BioRad). Images acquired were analyzed using Image Lab software (BioRad).
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bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mixture was transferred to an electroporation cuvette with a 1 mm gap width and transformed using a Gene Pulser Xcell electroporator (BioRad) with 1.8 kV ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Pathology 2022Quote: ... with 1% Triton X-100] and the protein concentration of each lysate was determined with a colorimetric assay (Bio-Rad). After mixing with SDS-PAGE sample buffer ...
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bioRxiv - Pathology 2022Quote: ... 1 or 10 μg of total protein were loaded and separated by SDS–PAGE on 10% mini gels (Bio-Rad), and transferred into a 0.2 μm-pore size nitrocellulose membrane (#GE10600001 ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies (for further specification see key resource table, table 1) were diluted in blocking solution (F4/80 [Bio-Rad] ...
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bioRxiv - Biochemistry 2019Quote: 40 µl of electrocompetent cells were transformed with 1 ng of the lesion-containing or lesion-free control plasmids together with 1 ng of pVP146 (transformation control) by electroporation (GenePulser Xcell(tm) from BioRad, 2.5kV – 25µF – 200Ω) ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS-PAGE gels used for this work were 10 % final concentration made with 30 % acrylamide/bis solution at a ratio of 37.5:1 (BioRad 1610158). Please note that SDS-PAGE analysis does not resolve polyP-induced shifts ...