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Citations for Bio-Rad :
3301 - 3350 of 4440 citations for Human Brain Derived Neurotrophic Factor BDNF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 1 µg of total RNA was reverse transcribed to cDNA by using an iScript kit (#1708891, Bio-Rad) on a Mastercycler (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... total protein concentration was measured using a protein assay (DC™ Protein Assay Kit II, BIO-RAD, 5000112). Activin A measurements were normalized to total protein levels ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA removal and cDNA synthesis were performed with iScript gDNA clear cDNA synthesis kit (Bio-Rad 1725035) by following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from xenoengrafted TA muscles using Aurum Total RNA Fatty and Fibrous Tissue kit (Bio-Rad) per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... anti-CD8-PerCP-Cy5.5 (clone CT8, Southern Biotech, LYNX Rapid PerCP Antibody Conjugation Kit, Bio-rad, Feldkirchen, Germany), anti-CD45-APC (clone UM16-6 ...
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bioRxiv - Genomics 2023Quote: ... and cDNA was generated from 500 ng of RNA input using the iScript cDNA Synthesis kit (BioRad, #1708891), according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 µg of RNA per sample using the iScript cDNA Synthesis Kit (Bio-Rad) or iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 100-300 ng of RNA was used for cDNA synthesis using iScript cDNA Synthesis Kit (Bio-Rad, 1708891) following the manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant protein extract was collected and protein concentration was determined using DC Protein Assay Kit (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Total cDNA was synthesized as per the manufacturer’s instructions using the iScript cDNA synthesis kit (Bio-Rad, USA). For expression analysis using real-time PCR ...
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bioRxiv - Cell Biology 2023Quote: Knockdown was validated by qPCR analysis: RNA was isolated using the Aurum Total RNA Mini Kit (Bio-Rad). RNA yield and purity were verified by absorbance at 260/280 nm (NanoVUE spectrophotometer ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated from 7.5 μg of RNA using the iScript™ Advanced cDNA Synthesis Kit (Biorad, 1725038), and 20-30 ng of cDNA was then used for quantitative PCR with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad, #1708891). This cDNA was then diluted 1:3 using ultrapure ...
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bioRxiv - Cancer Biology 2023Quote: ... 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad, #1708891). This cDNA was then diluted 1:3 using ultrapure ...
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bioRxiv - Physiology 2023Quote: ... The Numb immunoprecipitated material was analyzed by SDS-PAGE and gels stained with silver stain kit (Bio-Rad)
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bioRxiv - Neuroscience 2023Quote: ... and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad). Membranes were blocked for 30Lmin in 5% low-fat milk in TBS-Tween 20 0.1% at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®). Transfer and equal loading were evaluated by activation of stain-free gels by UV light ...
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bioRxiv - Plant Biology 2024Quote: ... Signal detection and quantification was achieved using the Clarity Western ECL substrate kit (Bio-Rad, Catalogue No.: 1705061), a ChemiDoc MP imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µg of RNA was used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcriptase-PCRs (RT-PCR) were carried out using the iScript RT Kit for cDNA synthesis (BioRad, 1708841). Where the final mix include 1000ng of RNA sample and 5µL of iScript RT-PCR mix with reverse transcriptase ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cDNA was reverse transcribed from 500ng of RNA using iScript IV VILO cDNA synthesis kit (Bio-Rad), with reactions incubated at 25°C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... They were loaded in 10% acrylamide gels (TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10%, Biorad), with protein ladder (NZYColour Protein Marker II ...
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bioRxiv - Pathology 2024Quote: ... Total RNA was transcribed reversely to cDNA using iScript™ cDNA Synthesis kits (Cat # 170-8891; Bio-Rad). Quantitative PCR was performed to quantify Lrp2 mRNA abundance in the kidney using SsoFast™ EvaGreen® Supermix kits (Cat # 172-5204 ...
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bioRxiv - Molecular Biology 2024Quote: ... HMGA2 fibroid (HMGA2F) expression was determined by RT-qPCR using the iScript Advanced cDNA Synthesis Kit (BioRad, #1725037) for the reverse transcription and HMGA2 PrimePCR primer (BioRad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quantification of mRNA levels was conducted via an iTaq Universal SYBR Green One-Step Kit (Bio-Rad). The assay was performed on an ABI StepOnePlus PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... We assessed library quantities using digital droplet PCR (ddPCR) with the Library Quantification Kit for Illumina TruSeq (BioRad). We diluted libraries to 2nM and performed paired-end sequencing using a HiSeq 2500 (Illumina).
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bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
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bioRxiv - Genomics 2024Quote: ... chr10:28,469,205-28,662,312 in EquCab3.0) were extracted from 10 ml bacterial cultures with the Quantum Prep Plasmid miniprep kit (BioRad), according to supplier instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred to ethanol-activated 0.2μm PVDF membranes using the Trans-Blot Turbo RTA Mini Transfer kit (#1704272) from BioRad. Each blot was blocked using 5%/0.1% BSA/Tween in TBS and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was generated using 1000 ng of total purified RNA following iScript cDNA synthesis kit (1708890, Bio-Rad) manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: RNA was extracted using the OMEGA total RNA kit and reverse transcribed using iScript reverse transcriptase (Bio-Rad). qPCR was performed using TB Green Premix Ex Taq (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... The total RNA was used in generation of cDNA using a reverse transcription kit (Bio-Rad, Hercules, CA) with gene-specific anti-sense primers ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad) using a mix containing 8 µl sample lysate ...
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bioRxiv - Cancer Biology 2021Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). Q-PCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein quantification was assessed using the BCA Protein Assay Kit (Pierce) or the RC DC Protein Assay (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Messenger RNA (mRNA) was converted to a single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad). qPCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... Molarity of the pool was quantified by qPCR using KAPA Library Quantification Kit and the CFX96 qPCR system (Biorad) before sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant were transferred to fresh tube and solubilized protein was measured using a DC protein assay kit (Bio-Rad). For immunoblotting an appropriate volume of 1 x Laemmli (Bio-rad ...
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bioRxiv - Cell Biology 2020Quote: ... Synthesis of complementary DNA (cDNA) was completed using 1 μg of RNA and the iScript cDNA synthesis kit (BioRad). Each qRT-PCR was performed as triplicate reactions with the following reaction mixture ...
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bioRxiv - Immunology 2021Quote: ... Approximately 400 pg of RNA was subjected to reverse transcription using the iScript RT kit (BioRad, Inc., Hercules, CA). Resulting cDNA products were included with 50nM VH1-VH6 specific primers and 250nM Ca ...
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bioRxiv - Microbiology 2021Quote: ... and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat. # 1708890, Bio-Rad). Real-time qPCR was conducted using the iTaq™ Universal SYBR® GreenSupermix (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 0.5 to 1μg RNA using SensiFast cDNA synthesis kit and qRT-PCR was performed using iTaq Universal SYBR Green Supermix (Biorad) (refer Purushothaman et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNAs were extracted by the Aurum ™ Total RNA mini kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subject to qRT-PCR assays by the Power SYBR® Green RNA-to-Ct™ 1-Step Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA synthesis was carried out using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA Cat# 1708890) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of total RNA was reverse transcribed with random primers using an iScript gClear cDNA synthesis kit (BioRad) and including a no-RT and controls for each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs was prepared using RevertAid First Strand cDNA Synthesis Kit (Thermoscientific) according to the manufacturer’s recommendation to perform qRT-PCR (BioRad) (Sup Table 1) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were quantified for protein concentrations using the Bio-Rad DC™ Protein Assay kit (Cat.5000112, BioRad) and loaded to a 10% SDS-PAGE gel ...