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Citations for Bio-Rad :
3201 - 3250 of 4172 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Viral RNA titers and infections were assessed via qRT-PCR using an iTaq Universal Probes One-Step Kit (BioRad) in a 20 µl sample volume ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Plant Biology 2024Quote: ... 500 ng of the purified RNA was employed to synthesize cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). RT-qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... using 11 μL undiluted RNA and the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Cat# 1864021). Droplets were generated ...
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bioRxiv - Cell Biology 2023Quote: ... following the procedure of Stoeckius et al.[28] using the LYNX Rapid Streptavidin Antibody Conjugation Kit (Biorad, Ref #LNK163STR). Briefly ...
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bioRxiv - Pathology 2024Quote: ... First strand cDNA was synthesized from 500 ng RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Real-time PCR was carried out using TaqMan assays in a 10 μl reaction mixture (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using candidate primers (Integrated DNA Technologies; Table 1) and an iScript cDNA Synthesis Kit (Bio-Rad). Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto membranes using Trans-Blot® TurboTM RTA Midi 0.2 µm Nitrocellulose Transfer Kit (Biorad, #1704271). The membranes were subsequently blocked in TBST solution (200 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... according to manufacturer’s protocol and used for reverse transcription with iScript cDNA synthesis kit (Bio-rad, cat #170-8890) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Microbiology 2023Quote: ... NS1 antigen levels were measured using the Platelia™ Dengue NS1 Antigen kit (Cat No. 72830, Bio-Rad, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... mRNA was isolated with TRIzol RNA Isolation protocol and used to make cDNA using iScriptTM cDNA Synthesis Kit (BioRad). The StepOneTM Real-Time PCR System was used to analyze the samples under the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5 μg total RNA was reverse transcribed to generate cDNA using the iScriptTM cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription PCR was performed on the sample to generate cDNA using iScriptTM cDNA Synthesis Kit (BioRad, Hercules, USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The cDNA obtained was subjected to qRT-PCR using the itaq™ Universal SYBR green kit (Bio-Rad, #1725122) and the CFX 96 Real-Time System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was carried out with 500 ng of total RNA using the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of RNA was used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad Cat# 1708891). qPCR was conducted using PowerUp™ SYBRTM Green Master Mix (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... A 500-ng aliquot of the isolated RNA was converted to cDNA with the iScript Advanced Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
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bioRxiv - Genetics 2023Quote: ... One μg of total RNA was reverse transcribed using a mix of oligo(dT) and random hexamer primers following the manufacturer’s instructions of the iScriptTM cDNA synthesis kit (BioRad). Primers to detect endogenous transcript levels of MSH2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplification of WHY1 for each Corallorhiza species was performed using the Taq RED PCR Kit from Bio-Rad Industries (Hercules ...
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bioRxiv - Genetics 2023Quote: ... Approximately 700 ng total purified RNA was used to generate cDNA with the iScript cDNA Synthesis kit (Bio-Rad). cDNA was used for PCR to quantify gene expression for SLC15A4 ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized from 1 µg of RNA using the iScript™ Reverse Transcription kit (Bio-Rad, USA). qRT-PCR was performed using gene-specific primers for eef1a1l1 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed using the SsoAdvanced™ Universal SYBR® Green RT-PCR Kit (Bio-Rad) on a CFX96 thermocycler (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: Cortical and hippocampal brain tissue sections were lysed using the Bio-Plex cell lysis kit (Bio-Rad Laboratories #171304011) and protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher #23225) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 ng of the extracted RNA was reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Complementary DNA was then synthesized from 1 μg of total RNA using iScript cDNA Synthesis Kit (1708890, Bio-Rad) and Bio-Rad T100 thermocycler ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug of RNA was diluted into 15 uL reverse transcription reactions using the iScript RT kit (Bio-Rad) and reactions were carried out following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Complementary DNA was synthesized from 0.5μg total RNA for all in vivo and in vitro experiments using the iScript cDNA kit (1708891, Bio-Rad). Five µL qPCR reactions were carried out using iTaq Universal SYBR Green Supermix (1725124 ...
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bioRxiv - Neuroscience 2023Quote: Fractionation of nuclear and cytoplasmic fractions from Drosophila lysates was conducted with the ReadyPrep Protein Extraction Kit (Bio-Rad). Seven whole flies per group were homogenized in cytoplasmic extraction buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Molecular Biology 2023Quote: ... the concentration was calculated by the Bradford assay using a Quick Start Bradford Protein Assay Kit 2 (Bio-Rad).
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bioRxiv - Genomics 2023Quote: ... and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, #1708890). We used RT-qPCR to quantify MYC expression (forward primer ...
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bioRxiv - Microbiology 2023Quote: ... Products were purified using the ssDNA clean and concentrate kit and loaded on 10% TBE-Urea gels (BioRad #4566033) with sample buffer (Thomas Scientific #C995V39 ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted and purified from RPE tissue punches using Single Shot Cell Lysis Kit (Bio-Rad Laboratories). cDNA synthesis was performed from 1.5 μg RNA using RT² First Strand Kits (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: qRT-PCR was performed on a C1000 Thermal Cycler using SsoFast™ EvaGreen Supermix kit (Bio-Rad, Hercules, CA) according to the instructions ...
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bioRxiv - Immunology 2023Quote: ... The protein bands were visualized using an ECL kit (Meilunbio, CHN) and imaged on ChemiDoc XRS+ (Bio-Rad, USA).
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bioRxiv - Cancer Biology 2023Quote: Retrotranscription of RNA was performed using 3μg of total RNA in a 40μl final volume reaction mixture using iScript Advanced cDNA Synthesis Kit for RT-qPCR (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The total RNA (1.2 μg) was used to synthesize single-stranded cDNA using iScript cDNA synthesis Kit (Bio-Rad) and T100 Thermal Cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... 500ng RNA was used as input for cDNA synthesis through iScript™ advanced cDNA synthesis kit (Bio-Rad 1725038) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was revealed with the Clarity™ Western ECL Substrates Kit (Bio-Rad Laboratories Inc, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using the iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories, Hercules, CA, USA) with 2.0-20 ng input corneal RNA according to a manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... The total protein content was determined using the colorimetric Bio-Rad Protein Assay Kit II (Bio-Rad Laboratories, CA). Seven dilutions of a protein standard containing 0 to 30 µg/mL of the total protein content were used ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA was then reverse transcribed using an iScript cDNA Synthesis Kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) following the manufacturers guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed using 350 ng (CD11B+) and 1,000 ng (brain) mRNA with an iScript cDNA synthesis kit (BioRad). Real-time quantitative PCR was performed on triplicate samples using SYBR Green Supermix (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Freshly isolated mRNA was reverse transcribed into complementary DNA with random primers by iScript cDNA Synthesis Kit (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Signal was detected using the HCL kit (ECLTM Primer Western Blotting Detection Reagent, AmershamTM) and a Chemidoc system (Biorad). Signal were quantified with the ImageLab Software.
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bioRxiv - Developmental Biology 2023Quote: ... total RNA samples were reverse transcripted into first-strand cDNA using the iScript Select cDNA Synthesis kit (Bio-rad). 1 µl of a 10X diluted cDNA sample was then used in the qPCR reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-rad) ...