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Citations for Bio-Rad :
3451 - 3500 of 4172 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... High integrity RNA (RIN > 7) was used to synthesize cDNA with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). PCR primers for human B-ACTIN ...
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bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 250-1000 ng of total RNA using an iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Quantitative PCR for each candidate gene was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were assessed using the Protein Assay BCA kit (Nacalai Tesque) with bovine serum albumin (Bio-Rad, Hercules, CA) as a standard ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA samples from monotreme tissues were reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). RNA was stored in nuclease free water at -80°C.
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bioRxiv - Immunology 2024Quote: ... RNA from 14 3h PBF guts was transcribed into cDNA with the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) and RT-PCR was performed with RedTaq Polymerase (VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA cDNA synthesis kit (BIORAD, CAT#1708891), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Conversion of one µg of RNA into cDNA was performed using the iScript cDNA Synthesis Kit (1708890; Bio-Rad Laboratories) and Master Cycler Pro Device (EPPE6324000.516 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membrane strips were finally washed 6x for 3mins in 1X TBST before being developed with the ECL Clarity Kit (BioRad) and imaged on ChemiDoc Imaging system (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA retro-transcription was performed using a High-Capacity cDNA Reverse Transcription Kit on a MyCycler (Bio-Rad, Hampton, NH). Next ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5%BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were resolved on 4-15% SDS-PAGE gels and transferred to nitrocellulose membrane (Trans-Blot Turbo RTA transfer kit, #1704271, Biorad). Membranes were blocked for 1h at room temperature in 5% BSA-TBS-T (TBS-0.1%Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... transferred to nitrocellulose membranes using semi-dry transfer apparatus and Tans-Blot Turbo RTA Midi Nitrocellulose Transfer kit (Bio-Rad). The membranes were then blocked with 3% milk in TBST ...
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bioRxiv - Microbiology 2023Quote: ... fed nymph and mouse bladder samples using the iScript Reverse Transcription supermix kit according to the manufacturer’s instructions (Bio-Rad). Parallel cDNA reactions were carried out in the absence of reverse transcriptase ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from sorted cardiac cells or from cardiac tissues (atria or ventricle) using the Aurum total RNA fatty and fibrous tissues kit (Biorad) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaqTM universal SYBR® Green One-Step kit (Bio-Rad): 95 °C for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... A 1.0 μg of total RNA was used for the cDNA synthesis using the iScript cDNA Synthesis kit (Bio-Rad). The qRT-PCR analysis was performed using CFX 384 Touch RT-PCR detection system with a reaction mixture of 2.0 μl of primer mix (2.0 μM from each F & R primer) ...
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bioRxiv - Physiology 2023Quote: ... 500 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad #1708891, Hercules, CA, USA) per the manufacturer’s instructions ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... interleukins and ghrelin were measured using Biolpex multiplex kits (cat# M50-0KCAFOY and cat # 171-A4S01M, Bio-Rad, CA, USA). Standards with known concentrations of specific analytes were reconstituted and diluted as instructed in the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... tagmentation and droplet library preparation were performed following the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... qPCR was performed using the SYBR Green Supermix Kit (Vazyme) on the CFX96 Real-Time PCR System (Bio-Rad, USA). The expression of target genes was normalized to expression of Gapdh ...
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bioRxiv - Microbiology 2023Quote: ... Separated proteins were then transferred to a nitrocellulose membrane using a Trans-Blot Turbo RTA Mini 0.2 μm Nitrocellulose Transfer Kit and Trans-Blot Turbo system (Bio-Rad). The membrane was cut to separate the regions above and below approximately 70 kDa ...
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bioRxiv - Microbiology 2023Quote: ... first-strand cDNA synthesis was prepared from 200 ng of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... We then utilized 1 µg of the total RNA for the SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad). rRNA depletion was performed with riboPOOL for D ...
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bioRxiv - Cell Biology 2023Quote: cDNA was prepared after extracting total RNA from young adult worms or cell extracts using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: 500-1000 ng of RNA were used for the retrotranscription reaction using iScript cDNA Synthesis Kit (BioRad, CA, USA, #1708890). Expression values were determined by q-PCR using Sybr Green (iTaq SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein concentrations of the supernatants were determined using DC™ Protein Assay Kit (Bio-Rad #5 000 112). Equal concentrations of protein (30 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from total purified RNA (1 μg) from each sample by using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 0,5 %) and protein amount was measured using a Lowry based method (DC Protein Assay Kit 5000111, Bio-Rad). 10μg protein per sample were subjected for protein digestion and peptide purification using the iST label free sample preparation kit (PreOmics GmbH) ...
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bioRxiv - Genomics 2022Quote: ... After incubation with HRP-coupled secondary antibody diluted in saturation buffer (1 h, room temperature), membranes were revelated using ECL kit (Pierce, 34076) on Chemidoc XRS+ imager (Biorad). Images were processed and analyzed using ImageJ software ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of one μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA was used to perform the reverse transcriptase reaction with iScript TM cDNA Synthesis Kit (Bio-Rad). Changes in mRNA levels of the target genes were determined by relative RT-qPCR with a CFX96TM Real‒Time PCR Detection System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... The spleen was removed and processed in tissue protein extraction reagent for cytokine analysis and protein quantification using a standard Bradford protein assay kit (Biorad). Subsequently tissues and serum were analyzed for cytokine levels using standard ELISA kits ...
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bioRxiv - Physiology 2023Quote: ... ∼300ng of purified RNA was revers transcribed into cDNA in the 20 μL using iScript cDNA synthesis kit (Bio-Rad). 2 μL of this reaction was used for subsequent qPCR reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed by spectrophotometry with PierceTM BCA Protein Assay Kit (ThermoFischer Scientific, cat # 23225) and proteins were separated on a 4% to 20% polyacrylamide gel (Biorad), and transferred to PVDF membrane (Bio-rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA concentration was quantified by spectrophotometry and used to prepare cDNA using the iScript cDNA Synthesis Kit (Bio-RAD), followed by the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and IFNγ) by the Bio-Plex Pro Mouse Cytokine Assay kit with the Bio-Plex 200 systems from Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2023Quote: The total protein concentration of the ECM digests was measured using a DC protein assay kit (Bio-Rad, Watford, UK) following the manufacturer’s instruction (n = 4) ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Neuroscience 2023Quote: 500ng of RNA/sample was used to make cDNA using iScript cDNA synthesis kit according to manufacturer’s protocol (Bio-Rad). cDNA abundance was measured using 300nM of indicated primers and a iQ5 qPCR system (Bio-Rad).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
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bioRxiv - Genomics 2023Quote: ... Two μg of total RNA each was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Oligonucleotide primers used for RT-PCR for each gene are listed in Supplementary Table S4 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was measured using the modified Lowry assay (DCTM Protein Assay Kit, BIO-RAD, 5000111). The same amount of total protein was mixed with Laemmli sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Plant Biology 2023Quote: ... leaf tissue was ground in liquid N2 and total RNA was extracted using the Aurum Total RNA Mini Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies conjugated with alkaline phosphatase were used with an ImmunStar™-Alkaline Phosphatase (AP) Western Chemiluminescent Kit (Bio-Rad) for signal development ...
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bioRxiv - Genetics 2023Quote: ... 800 ng of total RNA were used to perform the RT step using the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 μL ...