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Citations for Bio-Rad :
3201 - 3250 of 4217 citations for OX7 Anti Thy 1 Mouse Monoclonal biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 1 µg RNA using iScript gDNA Clear cDNA Synthesis Kit (BIORAD, Hercules, CA), according to manufacturer’s instructions and the cDNA was diluted to 5 ng/µl.
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized from 1 μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using the LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2023Quote: ... destained in 7.5% acetic acid for 1 minute and imaged using a ChemiDoc imaging system (Bio-Rad). For western blotting ...
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bioRxiv - Biochemistry 2022Quote: ... stock solution of 40% neutral acrylamide and N,N’-methylenebisacrylamide in a 19:1 ratio (Bio-Rad) were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt ...
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bioRxiv - Cell Biology 2023Quote: ... RNA (1 μg) was then converted to cDNA using an iScript cDNA Synthesis Kit (Bio-Rad: #1708891BUN), which was then analyzed on a BioRad CFX96 Real Time Thermocycler using 2X SYBR green qPCR master mix (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... and cDNA was prepared from 1 μg of RNA using the iScript cDNA synthesis kit (Bio-Rad), both according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... and resolved in a 1% LE agarose gel in 0.5x TBE using a CHEF-DRII instrument (BioRad) using the following settings ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized from 1 μg total RNA using iScript reverse transcription supermix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Detergent removal was initiated 1 hour after mixing all ingredients by adding 200 mg of BioBeads (BioRad) and mixing at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted 1:1000 in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA reverse-transcribed from 1 µg of this RNA using iScript Reverse Transcription Superrmix (Bio-Rad, 1708840) was used as a template for performing qPCR using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... 1 μg of RNA was reverse-transcribed to cDNA (Bio-Rad Real-time PCR system, Hercules, CA). Stat3 deletion or differential gene expression between experimental groups was confirmed by performing qRT-PCR in a CFX real-time PCR machine (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of total RNA was converted into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad). RT-qPCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... slides were heated at 37 °C for 1 min on a T100 thermal cycler (BioRad, Hercules, CA), transferred to precooled methanol (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... 1 μg of RNA was used for cDNA synthesis with iScript cDNA Synthesis Kits (BioRad, Hercules, CA) in a 20μl reaction volume ...
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bioRxiv - Molecular Biology 2024Quote: ... The levels of proteins bound to chromatin were quantified using Quantity One 1-D Analysis Software (BioRad) and normalized with their corresponding Histone H2B values ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated shaking for 1 hour at RT with 4% blocking solution (5% milk powder (BioRad) in TBS-T) ...
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bioRxiv - Molecular Biology 2023Quote: ... and individual band densities were measured and compared using Quantity One 1-D analysis software (Biorad, USA). The resulting values were normalized separately for each set of time points.
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bioRxiv - Cell Biology 2023Quote: ... The primary and secondary antibodies used were as follows: Collagen I (Bio-Rad 2150-1410, 1:300), α-SMA (Sigma A2547 ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were visualized using the VersaDoc Imaging System with Quantity One 1-D Analysis software (Bio-Rad).
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bioRxiv - Synthetic Biology 2024Quote: ... for 1 hour and de-stained in water overnight before imaging on a ChemiDoc Imager (Bio-Rad) or a GelDoc Go Imager (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: 6-well culture plates were coated with 1 ml of 0.6 % Low Melt Agarose (Bio-Rad; 1613111). Cells were diluted at a density of 1.0 x 104 cells/ml in media containing 0.3% Low Melt Agarose and seeded 1 ml/ well ...
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bioRxiv - Cell Biology 2024Quote: ... 0.3 to 2.5 µg plasmid DNA were mixed thoroughly with 50 µL 1 µm gold carriers (BioRad) (40 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 1 µg RNA samples using the iScript cDNA Synthesis kit (Bio-Rad, USA). qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min before incubating with an antibody against Collagen I (1:500, Bio-Rad, 2150-1908), Collagen III (1:300 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of RNA was used for reverse transcription using an iScript cDNA synthesis kit (Bio-Rad). cDNA was diluted 5 times in Milli-Q water and used as a template for PCR amplification using GoTaq G2 Green Master Mix (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of total RNA was converted into cDNA with iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and 50 ng of cDNA was used in each reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% agarose TBE gels and detected by trans-UV illumination using the Gel Doc XR+ (Bio-Rad) and Image Lab software (version 5.1) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from ∼ 1 μg of extracted RNA using an iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed for IFNB and IL-1β on the Applied Biosystems PRISM 7500 qPCR machine ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated with a 1:5,000 dilution of StrepTactin-horseradish peroxidase (HRP) conjugate (Bio-Rad), and 1:500 dilution of custom polyclonal antibodies (Cocalico Biologicals ...
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bioRxiv - Immunology 2022Quote: ... Nitrocellulose-bound primary antibodies were detected with anti-rabbit IgG horseradish peroxidase-linked antibody using the Clarity Western Peroxide and Luminol/Enhancer reagents (Bio-Rad Laboratories, CA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then washed thrice with TBST (5 minutes/wash) at room temperature and then incubated with HRP-conjugated goat anti-rabbit IgG (H + L) (Bio-Rad, Cat #: 170-6515) or goat anti-mouse IgG (H + L ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and qPCR was performed using the iTaqTM Universal SYBR® Green 1-Step Kit (BioRad 1725151) on a Bio-Rad CFX Connect Real-Time System ...
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bioRxiv - Cell Biology 2020Quote: ... TBST (50 mM Tris-HCl pH 7.4, 1% Triton X-100) with 5% non-fat milk (Bio-Rad) was used for blocking ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was used to make cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). All primer pairs were designed and validated in-house for efficiency and specificity ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of extracted RNA was transcribed into cDNA using the iScript Reverse Transcription Supermix kit (Biorad, 1708840). Quantitative RT-PCR was performed using the PerfeCTa SYBR Green SuperMix (Quantabio ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Tween 20) at room temperature for 1 hour and stained with 0.3 μg/mL streptavidin-HRP (BioRad) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Physiology 2019Quote: ... containing the pre-formed Cas9/sgRNA ribonucleoproteins and loaded into a 1-mm electroporation cuvette (BioRad, 165-2089). Electroporation was performed using the GenePulser Xcell (BioRad ...
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bioRxiv - Bioengineering 2019Quote: ... PAGE separation was carried out in 1 mm vertical mini gel format (Bio-Rad Mini-PROTEAN Tetra Cell) with a discontinuous buffer system under denaturing conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was generated from up to 1 μg of RNA with the iScript cDNA Synthesis kit (Bio-Rad). Quantitative PCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µL input and the entire IP fractions were reverse transcribed using the iScript cDNA synthesis kit (BioRad) and subjected to RT-qPCR ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were resolved on 1% agarose gels and imaged by ChemiDoc XRS+ system (Bio-Rad Laboratories). Image Lab™ Software 6.0.1 (BioRad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (SYBR® Premix Ex Taq™ ...
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bioRxiv - Physiology 2019Quote: ... Samples were then diluted to a final concentration of 1 μg/μl in 4x Laemmli buffer (Bio-Rad) and ultra-pure water and boiled for 10 minutes to denature proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 hr using a Trans–Blot device for semi-dry transfer (Bio-Rad Laboratories, Hercules, CA, USA). Membranes were then blocked for 1 hr at RT with 5% skim milk in 0,1 % PBS-Tween and incubated overnight at 4o with the following primary antibodies diluted in 5% milk ...
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bioRxiv - Genetics 2020Quote: ... The samples were then transferred at 0.25 A for 1 hour to a PVDF Membrane (Bio-Rad 1620177). Following protein transfer ...
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bioRxiv - Genetics 2021Quote: ... Samples were then diluted 1:5 and placed into a 96 well plate with Supermix (no dUTPs, BioRad) and the required target locus primers and MGB-NFQ probes (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was prepared from 1 μg of RNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). cDNA samples were diluted 1:10 and real-time PCR was performed using IQ SYBR Green supermix and gene-specific primers in the iCycler real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: 2 ⨯ 106 CAR T cells collected after 24-day expansion were resuspended with 1 ⨯ Laemmli sample buffer (BIORAD) containing 2.5% β-mercaptoethanol before sonication with 50% amplitude in Sonic Dismembrator (FisherBrand) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using iScript Reverse Transcription Supermix (1708840, Bio-Rad). Quantitative PCR was performed with 100 ng of cDNA ...