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Citations for Bio-Rad :
3101 - 3150 of 4217 citations for OX7 Anti Thy 1 Mouse Monoclonal biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 1 µg of RNA was used to synthesize cDNA with iScript™ cDNA Synthesis Kit (Biorad), and 100 ng cDNA was used as a template for the reaction ...
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bioRxiv - Biochemistry 2024Quote: ... 1-20 μg of total protein was resolved on a 4-20% polyacrylamide gel (BioRad, 4561096) at 100 V for 60-90 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed with TBST and incubated with HRP conjugated secondary antibodies (Bio-Rad, 1:10,000) at room temperature for 1h for ECL chemiluminescence detection system (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was done in a 1 mm cuvette using a Gene Pulser Xcell (BioRad, München, Germany) with a constant time protocol with input parameters 1.5 kV ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of C51 and F9 tumors stained with IgG (L19)-FITC (final concentration 2 μg/mL) and detected with a Rabbit anti-FITC (Bio-Rad; 4510-7804) and Donkey anti-Rabbit AlexaFluor 488 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 3% H2O2 for 15 min at room temperature and then incubated with goat anti-rabbit IgG-HRP (Bio-Rad 170-6515) secondary antibody diluted 1:300 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used were Immun-Star goat anti-rabbit (GAR)-HRP Conjugate (Cat # 170-5046, or Cat#170-6515; Bio-Rad Laboratories, USA). Clarity Western ECL Substrate (cat #170-5061 ...
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bioRxiv - Biochemistry 2023Quote: ... of the anti-GFP nanobody variants was measured by differential scanning fluorimetry (DSF) using a CFX96 Real Time PCR Detection System (Bio-Rad, Hercules, CA). A 96-well thin-wall PCR plate (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, cat#1708841), then diluted 10-fold and used directly as the template for quantitative real-time RT-PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2020Quote: ... Detection via secondary antibody was done with goat α-rabbit mAb (1:5000) conjugated to HRP (Biorad). Immunoblots were developed using the SuperSignal West Femto (Thermo ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM pH 8.0 by two cycles of centrifugal gel filtration (Biospin mini columns, 6,000 MWCO, Biorad). The nsp’s were buffer-exchanged into 250-500 mM NH4OAc ...
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bioRxiv - Microbiology 2020Quote: ... and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). % recovery was calculated as 2 raised to the adjusted input Ct minus IP Ct multiplied by 100 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as Plet1 (1:200; Nordic MUbio MUB1512P conjugated to Bio-Rad ReadiLink 633/655 1351005). Single-cell suspensions for ESCs and EPSCs undergoing ES-to-TSC reprogramming as well as control cell lines (ESC/EPSC day 0 samples ...
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bioRxiv - Developmental Biology 2021Quote: ... A total of 1 µg of RNA was reverse transcribed using iScript reverse transcription supermix (Bio-Rad). Real-time PCR was performed as triplicates on 2 ng of cDNA using SYBR Green master mix (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized from 1 μl/RNA sample using an iScript kit (Bio-Rad, catalog #1708891), and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription using the iScript cDNA synthesis kit (Bio-rad). The gene expression analysis was carried out using the SsoAdvancedTM Universal SYBR Green Supermix (Bio-rad ...
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bioRxiv - Molecular Biology 2019Quote: ... and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad) with following parameters ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were assessed using 6% polyacrylamide gel (made with 29:1 Acrylamide/Bis Solution, Bio-Rad) electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then pulsed with 1.8 kV in a 1-mm-gap cuvette (Bio-Rad, USA).
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bioRxiv - Bioengineering 2019Quote: ... and 1 μg of RNA was transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). Primer pairs MFS17/MFS18 ...
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bioRxiv - Microbiology 2021Quote: ... dried for 1 hour at 70°C on a BioRad Model 583 gel dryer (BioRad, Hercules, CA), and protein visualized with Coomassie staining ...
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bioRxiv - Cancer Biology 2020Quote: ... for smaller pellets 50 µl 1% SDS (Sodium Dodecyl Sulfate, Bio-Rad Laboratories Inc., Hercules, CA, USA) was used.
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of RNA was reverse transcribed into cDNA using the Reverse Transcription Kit (Bio-Rad, USA). qRT-PCR was carried out using SYBR Green (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 min and then imaged for 300 s using the ChemiDoc imaging system (Bio-Rad # 17001401). Dialyzed laminin was adsorbed directly to wells to generate a standard reference curve for quantitation ...
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bioRxiv - Bioengineering 2020Quote: ... 0.4% β-mercaptoethanol) per lane or 1 μL of strep-tagged unstained protein standards (Bio-Rad # 1610363) and run at 100 V for 2 h in Tris-HCl-Glycine-SDS running buffer ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 min and then imaged for 120 s using the ChemiDoc imaging system (Bio-Rad # 17001401). Blots were analyzed using ImageLab software (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a one hour incubation in the appropriate secondary antibodies coupled to peroxidase (1:1000, Biorad). Proteins were detected by enhanced chemiluminescence detection (ECL ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes for 1 hour using the Trans-Blot turbo (BioRad) system using standard conditions ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, #170-6515, Biorad or #12-349 ...
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bioRxiv - Microbiology 2020Quote: ... Data were quantified using a Personal Molecular Imager with Quantity One 1-D analysis software (Bio-Rad) and analyzed with GraphPad Prism 4 software.
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM EDTA) using the Mini-PROTEAN Tetra Vertical Electrophoresis cell (gel size 10×8 cm, BioRad). Each run was preceded by a 30 min pre-run at 300 V to warm the gel ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was retro-transcribed using the iScript cDNA Synthesis Kit and polydT primers (Biorad). The cDNA was amplified using the primers listed in S2 Table and the SsoAdvanced universal SYBR Green supermix (Biorad) ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: A 1 mL Ni-NTA resin in a glass gravity-flow column (Econo-Column®, BioRad, USA) was set up inside a Coy anaerobic glovebox with an atmosphere of 100% N2 ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at room temperature and developed with Clarity™ Western ECL substrate (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2021Quote: ... Quantitative Real-Time PCR (qRT-PCR) was carried out using SYBR green 1 chemistry (BioRad, Hercules, CA). Samples for qRT-PCR experiments were assayed in triplicate using the BioRad CFX96 Q-PCR machine ...
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bioRxiv - Bioengineering 2020Quote: ... we prepared a 1% w/v agarose solution (molecular biology grade, Bio-Rad Laboratories, Hercules, CA, USA) in PBS under boiling until complete dissolution ...
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bioRxiv - Cell Biology 2020Quote: ... by wet electroblotting for 1 h at 100 V using the Mini-PROTEAN Tetra system (Bio-Rad). Equal protein loading was assessed by amido black staining (0.02% (m/v ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant contained 2.5 mg proteins/ 1 ml (determined using Bio-Rad Protein Assay, Cat. No. 5000006). This protein fraction was used for co-immunoprecipitation (input) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 107 cells were electroporated (Gene Pulser Xcell System, Bio-Rad: 250 V, 500 mF, 0.4cm cuvettes) with the following plasmid vectors ...
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bioRxiv - Biochemistry 2022Quote: ... The αM and β2 subunits of recombinant Mac-1 integrin were resolved on 4-20% polyacrylamide (BioRad), gradient gels by SDS-PAGE and stained with Coomassie brilliant blue R250 ...
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bioRxiv - Biochemistry 2022Quote: ... gels were dried at 80 °C for 1 h in a gel dryer (Model 583, Bio-Rad), exposed to phosphorimager plates and signal acquired in Typhoon Trio™ Variable Mode Imager (GE Healthcare) ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 µg of total RNA was converted to cDNA using iScript Select cDNA Synthesis kit (Bio-Rad). Quantitative RT-PCR was performed in triplicate using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and specified primers (Table 1) on a CFX96™ Real-time-PCR System (Bio-Rad Laboratories, USA). The thermal profile used for RT-qPCR was 95 °C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... HRP signals were visualised by enhanced chemiluminescence (ECL) (Cytiva) (1:25000) and imaged with Chemidoc (Bio-Rad). Primary antibodies targeting GAPDH (Chemicon ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was prepared from 1 ug of purified RNA using the iScript cDNA Synthesis Kit (Bio-Rad) as stated in the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... we mounted embryos individually in a column of 1% (w/v) low-melt agarose (Bio-Rad 1613112) in distilled water ...