Labshake search
Citations for Bio-Rad :
3001 - 3050 of 3629 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... 1∼2 μg of total protein for each lane) was loaded on a precast 4-20% gradient polyacrylamide gel (PAGE, Bio-Rad: 4561096) and electrophoresed at a constant voltage of 200 V for 35 min ...
-
bioRxiv - Microbiology 2022Quote: We finalized the IPATS-BLV reaction in a 22-μl reaction mixture containing 14 μl of 2× ddPCR Supermix for Probes (Bio-Rad, #1863023), 909 nM of primers except the RPP30 primers (DRB3*016:01-forward ...
-
bioRxiv - Microbiology 2022Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
-
bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
-
bioRxiv - Biophysics 2022Quote: ... Samples were centrifuged for 2 min at 12,000 x g before being separated on 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 160 V for 60 min ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
-
bioRxiv - Neuroscience 2024Quote: ... the concentration of the resultant solution containing labelled αSyn was adjusted to 2 mg/mL and excess unbound dye was removed using Bio-Spin 6 size exclusion spin columns (Bio-Rad Laboratories). ATT565-αSyn was added to LUHMES cells on day six in vitro at a final concentration of 2 µM ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted four-fold and 2 µL was used for quantitative PCR (qPCR) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) and 500 nM of gene-specific qPCR primers (see Table S2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Isolated products were electrophoresed through at 2% agarose gel stained with ethidium bromide and imaged on the GelDoc imager and camera system (BioRad, Hercules, CA).
-
bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
-
bioRxiv - Microbiology 2024Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using the gDNA of purified viruses was performed in parallel with each experiment ...
-
bioRxiv - Biophysics 2024Quote: ... and incubated for 3 hours at 37 °C and 5% CO2 with GPMV-vesiculation buffer consisting of 25 mM methanol-free formaldehyde (FisherScientific, cat# 11586711) and 2 mM dithiothreitol (Biorad, cat# 1610610) dissolved in HBSS ...
-
bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
-
bioRxiv - Genetics 2023Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd.) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd.) ...
-
bioRxiv - Immunology 2023Quote: The detection of IgG ANAs in the serum of Ngfr KO and Bcl2 Tg mice was performed by IF in Hep-2 coated slides (Kallestad™ Bio-Rad) following manufacturer instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were added to a 2 mL bed of Ni-NTA agarose resin in a 20 mL column (Bio-Rad 7321010) equilibrated with wash buffer (50 mM Tris-HCl (pH 8) ...
-
bioRxiv - Cell Biology 2023Quote: ... Concentration of the RNA was determined by a NanoDrop UV spectrophotometer and 2 μg of RNA was loaded onto 15% Criterion-TBE-Urea polyacrylamide gel (Biorad, cat#3450093) for size selection ...
-
bioRxiv - Evolutionary Biology 2023Quote: Protein was separated by electrophoresis on 12% SDSCPAGE and transferred to a PVDF (polyvinylidene difluoride) membrane at 100 mA for 2 h using a Mini-PROTEAN Tetra system (Bio-Rad, USA). For detection of PpSerpin1 isoform proteins or PrPAP1 ...
-
bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane using the iBlot 2 device and membranes were blocked for 1 hour at RT in 5% (w/v) Blotting Grade Blocker/PBS (Biorad, #170-6404). GCase was detected using rb mAb to hGBA antibody (abcam ...
-
bioRxiv - Microbiology 2023Quote: ... Droplet Digital PCR (ddPCR) was performed to measure HSV-2 genomic DNA levels in DRGs using the QX100 droplet digital PCR system (Bio-Rad Laboratories). HSV-2 genomic DNA was quantified using the following gD primers/TaqMan probe set ...
-
bioRxiv - Molecular Biology 2023Quote: ... fractions with Afl RT were pooled and loaded onto a 2-ml Macro-Prep DEAE Resin (Bio-Rad, column CA, Hercules, USA) pre-equilibrated with a buffer C (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm nitrocellulose membrane for proteins larger than 35 kDa and to 0.1 µm nitrocellulose or 0.2 µm PVDF membranes for proteins smaller than 35 kDa for 30 min using Trans-Blot Turbo transfer system (Bio-Rad, 1704150) or for 2.5 h using wet transfer system (TG buffer ...
-
bioRxiv - Immunology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
-
bioRxiv - Molecular Biology 2024Quote: ... The relative number of cells in each well was evaluated after solubilization in 40 µL of 2% Triton X-100 by measuring absorbance at 595 nm in a microplate reader (Bio-Rad Laboratories). All determinations were carried out in triplicate.
-
bioRxiv - Biochemistry 2024Quote: Purified polar flagellins (2 µg) were separated by 12% SDS-PAGE as described previously [33] and stained with Coomassie Blue (BioRad, Madrid, Spain), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... using cDNA generated from 0.1-2 ng total RNA with the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad catalog # 1864021) according to the manufacturer’s protocol with reverse transcription at 42°C and annealing/extension at 60°C.
-
bioRxiv - Microbiology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
-
bioRxiv - Evolutionary Biology 2019Quote: ... 2 ng of cDNA were used in each qPCR reaction on a CFX Connect Real-time PCR detection system (Bio-Rad, Hercules, CA) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of C51 and F9 tumors stained with IgG (L19)-FITC (final concentration 2 μg/mL) and detected with a Rabbit anti-FITC (Bio-Rad; 4510-7804) and Donkey anti-Rabbit AlexaFluor 488 (Invitrogen ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The blood was first separated from the ethanol through gentle centrifugation and embedded in molten 2% agarose plugs (Bio-Rad DNA plug kit). The solidified plugs were submerged in Lysis Buffer solution (Bionano ...
-
bioRxiv - Cell Biology 2021Quote: ... were amplified using Phusion High-Fidelity PCR MasterMix (primer sequences are listed in Additional File 2) and a C1000 thermocycler (Bio-Rad Life Science). The peak sequences were amplified for 98 °C for 5 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Reverse Transcription (RT) was performed with 2 µg RNA per 40 µl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
-
bioRxiv - Biophysics 2022Quote: ... the samples were cooled on ice for 10 min followed by the addition of 80 mg Bio-beads SM-2 Resin (Bio-Rad, Hercules, CA), then rotated at 4°C overnight ...
-
bioRxiv - Biophysics 2022Quote: The purified proteins of V2HeR3 were reconstituted into a mixture of POPE and POPG membranes (molar ratio = 3:1) with a protein-to-lipid molar ratio of 1:20 by removing DDM using Bio-Beads (SM-2; Bio-Rad, CA, USA). The reconstituted samples were washed three times with 1 mM NaCl and 2 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Cancer Biology 2022Quote: ... ddPCR was used to detect DNA of SP by using a QX100 Droplet Digital PCR System and 2× ddPCR Supermix (Bio-Rad, California, CA) with protocols developed in our previous studies 48-57 ...
-
bioRxiv - Cell Biology 2022Quote: ... and Reverse Transcription (RT) was performed with 2 μg RNA per 40 μl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
-
bioRxiv - Genetics 2022Quote: ... a small implant of the thallus of interest (0.5 cm2) was set in 2 mL tube containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
-
bioRxiv - Pathology 2019Quote: ... this amount of protein was broken down via a precipitation and purification kit (ReadyPrep™ 2-D Cleanup Kit, Bio-Rad, Warsaw, Poland). The obtained protein pellets were then dissolved in a rehydration buffer ...
-
bioRxiv - Immunology 2020Quote: ... The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories, USA) with 2 mM ATP (MP Biomedicals ...
-
bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2021Quote: ... and then liposomes were formed by the step-wise removal of pentaethylene glycol monodecyl ether by five additions at 20 min intervals of 60 mg Bio-Beads SM-2 (Bio-Rad, Watford, UK) with gentle mixing at 4 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Round spermatids were sorted based on 1C DNA content and size using a 2-laser sorter (BioRad S3e with 488nm and 561nm lasers) gated on single cells as previously described (Lesch et al. ...
-
bioRxiv - Biophysics 2021Quote: ... The proteins were resolved by SDS-PAGE and transferred to a PVDF membrane by tank blotting at 150 mA for 2 h using the Miniprotean III System from Bio-Rad (Hercules, USA). The membrane was blocked for 1 h with 5% skim milk in PBS containing 0.05% Tween-20 ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38) before running on a CFX96TM real-time PCR cycler (BioRad, Hercules, CA, USA) under cycle conditions of 42°C for 5 min ...
-
bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer: 2% Blotting Grade Blocker (Bio-Rad cat. # 1706404) and 2% heat-inactivated goat serum (Gibco ...
-
bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...