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Citations for Bio-Rad :
2801 - 2850 of 3629 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Incubation with appropriate HRP-conjugated secondary antibodies (Thermo) was for 2 hours at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD). Densitometric analysis was performed using ImageJ software (NIH).
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bioRxiv - Synthetic Biology 2021Quote: ... reactions were analyzed by urea-PAGE 2 μL of the reaction was mixed with 12 μL of TBE-Urea (Bio-Rad) loading dye and boiled for 10 minutes at 100 °C ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were resolved electrophoretically on ethidium bromide stained-2% agarose gel immersed in 1× TAE and visualized with Biorad Gel Doc XR instrument (Biorad, USA).
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bioRxiv - Microbiology 2020Quote: ... and placed on ice for 2 min before loading on a 10% denaturing urea polyacrylamide gel in TBE buffer (Bio-Rad) next to an RNA marker (low range ssRNA ladder ...
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bioRxiv - Microbiology 2021Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 μg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Neuroscience 2020Quote: ... 2 μl of brain homogenate from each sample was analyzed for protein concentration using BioRad protein assay reagent (BioRad, Hercules, CA). 1 μl of 0.2 M perchloric acid per sample was added to the remaining homogenate and was centrifuged at 10,000 rpm for 10 minutes at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The DDM/CHS detergent was then removed by overnight incubation with Bio-Beads SM-2 (15 mg per 1 ml protein/detergent/amphipols mixture, Bio-Rad) at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... DIV15-30 neurons were transfected 2-6 days before imaging via biolistic gene transfer (160 psi, Helios gene gun, Bio-Rad), as described in a detailed video protocol (Woods and Zito 2008) ...
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bioRxiv - Cell Biology 2022Quote: ... 25-50 follicles were collected per sample and boiled in sample buffer (Laemmli Sample Buffer, Bio-Rad + 2-mercaptoethanol, Bio-Rad). Samples were run on 4-15% gradient gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 25-50 follicles were collected per sample and boiled in sample buffer (Laemmli Sample Buffer, Bio-Rad + 2-mercaptoethanol, Bio-Rad). Samples were run on 4-15% gradient gels (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... detergent was removed from the sample by the gradual addition of 400 mg/mL SM-2 polystyrene Bio-Beads (Bio-Rad) over the course of four hours ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% (v/v) H2O2) were prepared and mixed followed by incubation for 2 min for chemiluminescence detection with ChemiDocTM MP System (BIO-RAD).
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bioRxiv - Genetics 2022Quote: ... The entire sample was loaded on 1.5 mm 8% SDS-PAGE gel and ran at 85 volts for 2 hours and transferred to PDVF membrane via semidry transfer (Bio-Rad) at 19 V for 2 hours and 30 minutes ...
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bioRxiv - Genetics 2022Quote: ... The samples were boiled at 65°C for 10 minutes and then loaded on 10% SDS-PAGE gel and ran at 90 volts for 2 hours and transferred to a PDVF membrane via semidry transfer (Bio-Rad) at 13 V for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 0.2 μm nitrocellulose membranes were prewet for 10 minutes in TBS then secured into the Bio-Dot Microfiltration apparatus (Bio-Rad). Wells were rehydrated once with TBS ...
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bioRxiv - Neuroscience 2022Quote: ... was performed for 2 h at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD).
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bioRxiv - Cell Biology 2022Quote: ... The PCR product was separated in a 2% agarose gel and imaged by ChemiDoc™ Touch Imaging System (Bio-rad Laboratories).
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bioRxiv - Plant Biology 2021Quote: ... and 2 μg of the RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative RT- qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad) were added and incubated for 16 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad) were added and incubated for 16 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2021Quote: ... Primers (Supplementary Table 2) were designed using Primer3 Plus (24, 25) to quantify transcripts using Universal SYBR Green Supermix (Bio-Rad) using a Quantstudio 6 Flex (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was diluted 1:4 in nuclease free water and each cDNA was combined with segment specific primers (Supplementary Table 2) and Precision Melt Supermix (Bio-Rad) and analyzed by qPCR in a CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... 500 nM concentration of each PCR primer and 2 μL of 15x diluted cDNA templates using CFX96 real-time PCR system (Bio-Rad) as described (Gayomba et al. ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The reactions were assembled isothermally on a PTC-225 Peltier Thermal Cycler (MJ Research) or a Tetrad 2 Peltier thermal cycler (Bio-Rad) at a suitable growth temperature (e.g ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions or ISVPs (2×1012 particles/ml) were incubated for 5 min at the indicated temperatures in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNA from Astyanax embryos of various stages (2-24 hpf) was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad) and amplified using the following primers:
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bioRxiv - Zoology 2020Quote: ... Samples were incubated in a 1:1 (w/v) ratio of 2× sample buffer (0.2M DTT, BioRad Laemmli Sample Buffer #1610737) at room temperature for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with SINV at an MOI of 2 and pictures were taken 6 hours post-infection using ZOE fluorescent cell imager (Bio-Rad). Proteins were collected with lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... to nitrocellulose membranes using a constant voltage of 100 V for 2 h using the BioRad Midi PROTEAN Western blotting module according to the manufacturer’s instructions (BioRad, CA, USA). The membranes were blocked with blocking solution [Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was transformed into Escherichia coli SS320 cells by electroporation in a 2-mm cuvette using a Gene Pulser Xcell (Bio-Rad) with a setting of 2,400 volts ...
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bioRxiv - Biochemistry 2021Quote: ... Proteoliposomes or protein-free liposomes were generated by two rounds of treatment with 300 mg of wet SM-2 Bio-Beads (Bio-Rad) in total ...
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bioRxiv - Neuroscience 2021Quote: ... In a water bath samples were boiled for 2 minutes and 12 ul of each lysate were loaded on two separate gels (Biorad, #4568096), one each required for parallel HTT and pS13 HTT detection ...
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bioRxiv - Microbiology 2020Quote: ... Optimized ddPCR was used to detect the presence of SARS-CoV-2 viruses following our previous study.10 Analysis of the ddPCR data was performed with QuantaSoft software (Bio-Rad). The concentration reported by the procedure equals copies of template per microliter of the final 1x ddPCR reaction ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... The Ct value was determined by CFX Maestro software when the fluorescence rises exponentially 2-fold above background (Biorad, Hercules, CA.). The reference group was the negative control birds.
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bioRxiv - Neuroscience 2021Quote: ... Gels were run for 2 hours and 10 min at 80V and transferred using the Trans-Blot SD Semi-Dry Transfer cell system (Bio-Rad). After transfer the LI-COR Revert 700 Total Protein Stain (LI-COR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... or in transfer buffer (25 mM Tris, 200 mM glycine, 20% ethanol) for 2 h at 52V with a liquid transfer system (Bio-Rad). Total proteins were revealed by Pierce® Reversible Stain (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 μg of RNA was used to synthesize cDNA with Bio-RAD iScript cDNA Synthesis Kit (170-8891, Bio-Rad). qRT-PCR was performed using Taqman universal PCR mix and predesigned gene expression assays with best coverage from Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins (10μl solubilized in 2X Laemmli/20% of 2-mercaptoethanol) were separated by SDS-PAGE on 10% polyacrylamide gel (Bio-Rad, Hercules). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixture was transferred to a pre-chilled 2 mm gap electroporator cuvette and cells were electroporated using the EC2 program (MicroPulser, Bio-Rad). Cells were then suspended in 3 ml 1/4 TSB (at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Three more washing steps of 2% w/v TBST/milk were applied and finally the membranes were screened with a ChemiDoc MP Imaging system (Biorad, USA) and freshly mixed ECL fluorescent mixture and optimal exposure times ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...