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Citations for Bio-Rad :
3001 - 3050 of 8863 citations for 7 Bromo 3 4 dihydro 1H benzo e 1 4 diazepine 2 5 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... was then added and the solution was mixed at 4°C for 10 minutes before addition of 100 mg of Biobeads SM2 (Bio-Rad). Biobeads were washed into methanol ...
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bioRxiv - Molecular Biology 2020Quote: ... aliquots of the supernatant and resuspended pellet fractions were separated by SDS-PAGE using 4-15% gradient TGX gels (Bio-Rad) and analyzed by densitometry using Image Lab software (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins associated with half of the Dynabeads were eluted with 22 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) by boiling at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The α-FN-EDA Fab was verified as >90% pure by SDS-PAGE (performed on 4-20% gradient gels (Bio-Rad)).
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bioRxiv - Molecular Biology 2021Quote: ... were separated by SDS PAGE in 4-12% Bolt Bis-Tris Plus gels (ThermoFischer Scientific) and transferred to 0.2 µm Immun-Blot® PVDF membrane (BIO-RAD). After blocking with 5% (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... The denatured samples were resolved on a 4-20% Mini-PROTEAN TGX Stain-Free precast protein gel (Bio-Rad, cat# 4568094) and transferred to a nitrocellulose membrane (0.45 μm ...
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bioRxiv - Neuroscience 2019Quote: ... Protein samples were electrophoresed on NovexTM 4-20% Tris-Glycine gels (Thermo-Fisher) and transferred onto nitrocellulose membrane using the CriterionTM Blotter transfer system (Bio-Rad). Membranes were blocked in 5% milk in .1% Tween/Tris-buffered saline (TBST ...
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bioRxiv - Genetics 2019Quote: ... and 1x phosphatase inhibitors and run as previously reported (Blauwendraat et al. 2018a) on 4-20% Criterion TGX gels (Bio-Rad) in Tris-glycine running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... paraffin-embedded sections were incubated at 4°C overnight with antibodies for F4/80 (MCA497G, Bio-Rad Laboratories, Hercules, CA, USA), HILPDA (sc-137518 HIG2 Antibody (C-14) ...
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bioRxiv - Pathology 2019Quote: ... 20% methanol and MilliQ water) overnight at 4°C in a wet transfer apparatus at 70 mA constant voltage (Bio-Rad). Detection of the chemiluminescence immunochemical reaction was performed on a ChemiDoc ™ XRS + Molecular Imager® (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins and/or immunocomplexes were separated by electrophoresis on a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed with anti-HA-HRP (3F10 ...
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bioRxiv - Biophysics 2019Quote: ... Temperature was increased at a rate of 1°C per minute from 4°C to 100°C and fluorescence was measured every minute with a qPCR thermocycler in FRET mode (Bio-Rad). Aggregation temperature was determined by locating the global minimum of the second derivative of the raw data ...
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bioRxiv - Microbiology 2019Quote: ... in a 4 mm cuvette and then electroporated at 960 μF and 250 V with a Gene Pulser Xcell system (Bio-Rad). Electroporated cells were plated into 10 cm plates at 2 × 105 ...
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bioRxiv - Physiology 2019Quote: ... protein samples (20 µg of total protein) were separated at 300 V for 30 min using 4-20% gels (Criterion TGX, Bio-Rad), followed by wet transfer to PVDF membranes (0.2 µm Immun-Blot ...
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bioRxiv - Physiology 2020Quote: ... equal amounts (30 μg) of protein were resolved on 4–20% TGX stain-free gels (Bio-Rad Laboratories GmbH, Munich, Germany) and transferred onto PVDF membranes ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% CriterionTM TGXTM precast Midi protein gel (Bio-Rad) for SDS-PAGE followed by wet transfer to nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2019Quote: ... Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093, Bio-Rad) and transferred to PVDF membrane (1620177 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Protein concentration was quantified using a BCA assay and loaded at 25- 50ug in 1x Lamelli Buffer with B-mercaptoethanol onto a 4-15% TGX protean gel (Bio-Rad). In some experiments to achieve greater separation to detect the N-truncation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: 40-60μg of protein was electrophoresed through 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hertfordshire, UK) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Genetics 2020Quote: ... Fractions of 200 μl were collected and analyzed in a precast 4-20% gradient SDS-PAGE gel and visualized using ImageLab™ (BioRad).
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bioRxiv - Genetics 2020Quote: ... Fractions of 250 μl were collected and analyzed on a precast 4-20% gradient SDS-PAGE gel and visualized using ImageLab™ (BioRad). A size calibration curve was previously prepared using thyroglobulin (670 kDa) ...
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bioRxiv - Cancer Biology 2020Quote: ... 20µg total protein in βmercaptoethanol-containing loading buffer was added per well onto 4-12% SDS-PAGE gels (NuPAGE) and transferred to PVDF (Bio-Rad) or nitrocellulose (Amersham ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The purified proteins were separated by SDS/4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA), then transferred to an Immobilon-FL PVDF membrane (Merck Millipore Ltd. ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated using SDS-polyacrylamide gel electrophoresis (SDS-PAGE-4-12%) and transblotted onto nitrocellulose membranes (Bio-rad, California, USA). The membranes were blocked with 5% dry milk (overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heat denatured and equal concentrations of protein were electrophoresed on 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad) and transferred onto 0.2 µm PVDF membrane in Tris-Glycine transfer buffer containing 20 % methanol ...
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bioRxiv - Bioengineering 2020Quote: ... Equal amounts of protein lysates (10-20 μg total protein) were subjected to 4–20% SDS-PAGE (Bio-Rad, Hercules, CA). Western analysis was carried out with primary antibodies against phosphorylated Smad1/5 (p-Smad1/5) ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of the protein samples were separated on 4-20% SDS-PAGE (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) at 20 mA and 250 V as the limiting voltage.
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer B + 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer D ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer C + 400 mM NaCl ...
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bioRxiv - Pathology 2021Quote: ... maximum power with 30 second intervals and the lysate obtained by ultracentrifugation at 100,000 g for 60 minutes, 4°C (CS 100FNX, HITACHI) resuspended in PBS and the concentration was measured by Bio-Rad protein assay dye reagent concentrate (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... quantified by micro-BCA assay (Pierce) in duplicate extracts) on 4-20% gradient Bis-Tris polyacrylamide stain-free pre-cast gels (Bio-Rad) in denaturing conditions ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad®) for separation (200 V ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were subjected to westernblot analysis using precast polyacrylamide gels (10-20% Tris-HCl or 4-12% Bis-Tris, Bio-Rad). For immunoprecipitation (IP ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were washed and boiled in sample buffer and resolved on 10% SDS-PAGE or gradient gels (4 – 20%, Bio-Rad) and electro-transferred to PDVF membrane ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 μg of protein was loaded into each lane of a TGX-stain-free pre-cast 4–20% SDS gel (Biorad, 4568094), total protein was quantified upon stain-free gel imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amount of proteins from each lysate were subjected to polyacrylamide gel (4-10 %) electrophoresis (PAGE) in Mini-PROTEAN Tetra cell apparatus (Bio-Rad). PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad). The column bed was washed with 40 ml of a wash buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 1000xg to pellet the beads and the supernatant was loaded into a Mini-PROTEAN TGX 4-20% (w/v) gradient gel (Bio-Rad) to run for 45 minutes at 150V ...
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bioRxiv - Neuroscience 2021Quote: ... separated on a 4–12 % SDS/PAGE gel and then transferred using the Transblot®-Turbo™ Transfer system (Bio-Rad). After 1 hour blocking in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of total protein (20-60 µg per lane) was resolved by SDS-PAGE using Mini Protean TGX precast gels 4% to 20% (Bio-Rad). Immunoblotting by semi-dry transfer (Bio-Rad Trans-Blot SD semidry transfer cell ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg per lane of total protein samples were separated on 4-12% Bolt Bis-Tris Plus Gels and blotted onto polyvinylidene difluoride membrane (Bio-Rad) using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... or the eluates were separated on a 4-15% gradient sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pre-casted gel (Bio-Rad). For Mn2+-Phos-tag SDS-PAGE gel ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were eluted in the denaturing mode and run in a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad) at 200V for 30 min and stained by Imperial protein stain (ThermoFisher ...