Labshake search
Citations for Bio-Rad :
2951 - 3000 of 8863 citations for 7 Bromo 3 4 dihydro 1H benzo e 1 4 diazepine 2 5 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... Cell lysates were separated by SDS-PAGE 4 - 12 % gradient Bis-tris gel (Novex) followed by protein transfer to a PVDF membrane (BioRad) and antibody incubation ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
-
bioRxiv - Cell Biology 2024Quote: ... bound proteins were eluted with 2x Laemmli Sample Buffer and samples were then separated via SDS-PAGE using 4-20% Criterion TGX gels (BioRad) and transferred to a PVDF membrane ...
-
bioRxiv - Immunology 2024Quote: ... 20μg sample per lane were loaded onto 4-20% precast polyacrylamide gel membrane and then transferred onto nitrocellulose membranes (BioRad). Membranes were labeled with anti-DDX41 (Novus Bio) ...
-
bioRxiv - Microbiology 2019Quote: ... Relative fluorescence was measured at 5-minute intervals for 2 hours in a CFX96 system (Bio-Rad).
-
bioRxiv - Developmental Biology 2019Quote: ... 2 μl of gene-specific primer pair and 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad). The relative transcript abundance of each gene (normalized to rpl4 ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 μl of diluted vRNP mixtures was loaded onto a 5% polyacrylamide native TBE gel (Bio-Rad) and run at 125 V for 80 min at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... cells were stimulated with 5 μg/ml anti-Igκ plus anti-Igλ F(ab’)2 antibodies (BioRad). After 5 min of data acquisition ...
-
bioRxiv - Plant Biology 2021Quote: ... The binary vector pMGE599 was introduced into Agrobacterium tumefaciens AGL-1 strain (62) through electroporation (E. coli Pulser; Bio-Rad, http://www.bio-rad.com). The scutella tissue of barley caryopsis was used for Agrobacterium-mediated transformation as described previously (63) ...
-
bioRxiv - Molecular Biology 2019Quote: ... using transfer buffer containing27mM Tris-Cl,48mM glycine,20% methanol for 1h in a Trans-Blot semidry electroblotter (Bio-Rad). Blots were blocked overnight with 5% skim milk and incubated with primary antibodies in PBS containing 1% bovine serum albumin ...
-
bioRxiv - Neuroscience 2022Quote: ... 20% DMSO and 0.3M Glycine for 1h followed by a blocking step using an aqueous solution of 0.2% Triton X100 (Bio-Rad Laboratories), 0.2M Tris pH8 ...
-
bioRxiv - Cell Biology 2023Quote: ... And then blots were probed with secondary antibodies for 1h at room temperature before ECL development and imaging (Bio-Rad). The primary antibodies used for immunoblotting are as follows ...
-
bioRxiv - Microbiology 2022Quote: ... 7 μL of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and RNase-free water for a final reaction volume of 15 μL in each well ...
-
bioRxiv - Developmental Biology 2022Quote: ... 7×8.5 cm precut nitrocellulose membranes (BioRad, cat. #162-0146), at 4°C with magnetic stirring and an opposing cold-pack ...
-
bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was conducted using the QuantumStudio-7 (Bio-Rad). mRNA expression levels were quantified by real-time PCT using SYBR green fluorescence ...
-
bioRxiv - Neuroscience 2024Quote: ... for 7 min using Trans Blot Turbo System (Bio-Rad). Filters were washed three times and blocked for 1 hour in Tris-Tween buffered saline (TTBS ...
-
bioRxiv - Neuroscience 2024Quote: ... we used Image Lab software (BioRad, Version 6.1.0 build 7) to analyze the total volume of bands of interest and subtract the volume of a membrane space where no bands were present (background) ...
-
bioRxiv - Biochemistry 2020Quote: ... The purified MCU-EMRE subcomplex in DDM was then reconstituted into nanodisc by incubating with Msp1 protein, lipids (POPC:POPE:POPG, 3:1:1 molar ratio) and BioBeads (BioRad) at 4°C ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We used 1 μL diluted cDNA (1:3 in ddH2O) and iTaq Universal SYBR Green Supermix (Bio-rad) in the Bio-rad CFX96 real-time PCR detection system for qPCR experiments ...
-
bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
-
bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
-
bioRxiv - Cell Biology 2021Quote: ... were incubated in TBS-T/milk for 45 min and washed 3 × 5 min with TBS-T before detecting using a Chemidoc imager (Bio-Rad). NearIR-conjugated secondary antibodies (LI-COR Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
-
bioRxiv - Molecular Biology 2022Quote: ... membranes were washed in 1X PBS with 0.1% Tween 3 times for 5 minutes each before scanning using the ChemiDoc™ MP Imaging System (Bio-Rad). Note ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times for 5 min with TBS and were subsequently incubated with Clarity Western ECL substrate working solution (Bio-Rad) for 5 min ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed 3 times with TBST for 5 min each and then the membrane was incubated with Clarity Western ECL Substrate (Bio-Rad) for 2 min before exposure to film and development in a darkroom.
-
bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
-
bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
-
bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out with 3-5 μL of the linear PCR product (metH homology extension-flanked kan cassette) at 2.5 kV (Bio-Rad MicroPulser), and cells were immediately recovered with 1 mL SOC and cultured at 37°C for one hour ...
-
bioRxiv - Biophysics 2020Quote: ... before being transferred to Whatman 3 MM paper and dried (50°C, 2 hours) using a gel dryer (Model 583, BioRad) attached to a vacuum pump ...
-
bioRxiv - Microbiology 2020Quote: ... Cell lysates were mixed with Bradford reagent (1:5; Bio-Rad) and absorbance was measured at 595 nm ...
-
bioRxiv - Molecular Biology 2024Quote: ... were used at 1:5000 in 5% Blotting-Grade Blocker (BioRad).
-
bioRxiv - Biochemistry 2019Quote: ... 25 mg ml−1 Bio-Beads SM-2 (Bio-Rad) was added and incubated at 4°C for 1 h ...
-
bioRxiv - Biochemistry 2020Quote: ... diluted 2:1 in 4X Laemmli sample buffer (Bio-Rad) containing 100 mM Dithiothreitol (CAS No ...
-
bioRxiv - Microbiology 2022Quote: ... Capacitors were 1-cm Tygon tubing (2 mm ID, BioRad), having Luer connectors on each extremity ...
-
bioRxiv - Microbiology 2023Quote: ... and β-tubulin (clone YL1/2, Bio-Rad; 1:5000) antibodies at 4°C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
-
bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
-
bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
-
bioRxiv - Cancer Biology 2021Quote: Protein samples from LNCaP cells used to measure luciferase activity (described in Fig. 4A) were loaded into 4% to 15% SDS-PAGE (Bio-Rad) and subjected to electrophoretic analysis and subsequent blotting ...
-
Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
-
Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred to new tubes and 20 µL of the sample were loaded onto a 4-20 % gradient SDS-PAGE gel (Bio-Rad) and analyzed by immunoblotting.
-
bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad, Hercules, CA), and then transferred to PVDF membranes ...
-
bioRxiv - Genetics 2021Quote: ... 150 μg of protein was then separated on Criterion Precast Gradient 4-15% SDS-PAGE gels (Bio-Rad, cat. number 5671083) and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution ...
-
bioRxiv - Molecular Biology 2021Quote: ... Ten to twenty micrograms of denatured protein was loaded onto a 4-20% TGX stain-free polyacrylamide gel (Bio-Rad, USA) or NuPAGE 3-8% Tris acetate gel and subsequently transferred onto pre-cut nitrocellulose membranes ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... SDS PAGE was performed using 7.5% or 4-20% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023/4561094) run in running buffer (25 mM Tris ...
-
bioRxiv - Developmental Biology 2021Quote: ... 15µg of each sample was loaded per lane of a MIDI 4-15% Criterion Precast Midi SDS page gel (BioRad #5671085) and electrophoresis was performed at 120mV for 75 minutes ...
-
bioRxiv - Developmental Biology 2020Quote: Equal amounts of protein were resolved on either a 15% or a 4-20% Tris-glycine gradient SDS-PAGE gel and transferred to a polyvinylidene difluoride Immobilon-FL membrane (PVDF; Bio-rad). Membranes were blocked for 30 min at room temperature with 5% BSA in TBST (50 mM Tris ...
-
bioRxiv - Cancer Biology 2022Quote: ... 15-25 μg of proteins were loaded onto 4-12% Mini-PROTEAN Tris-Tricine Precast Gels or Criterion Tris-Tricine Precast Gels (Bio-Rad), and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science) ...