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Citations for Bio-Rad :
251 - 300 of 506 citations for IL 8 CXCL8 Human 77a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: All analytical gels were performed in 20% polyacrylamide gels (acrylamide:bisacrylamide 19:1) using 8×12 cm plates in a mini-PROTEAN system (BioRad). Acrylamide solution was prepared in 1X TBE buffer (89 mM Tris ...
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bioRxiv - Genomics 2019Quote: ... we first assembled the 8-strip tubes into 96-well plates for increased throughput (Bio-Rad, RC9601 and MSA5001). For FACS-collected cells we proceeded directly with the obtained 96-well or 384-well plates ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Cancer Biology 2022Quote: ... The reaction mixture was separated on an 8% native polyacrylamide gel and visualized using a ChemiDoc Imaging system (BioRad) with SYBR Green detection filter.
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bioRxiv - Microbiology 2022Quote: ... reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100; BioRad, Australia) and used immediately ...
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bioRxiv - Cancer Biology 2023Quote: ... and resolved by electrophoresis on a 4-20% or 8-16% polyacrylamide gradient gel (Mini-Protean TGX, Bio-Rad). Proteins were transferred to nitrocellulose or PVDF membranes (Immobilon-P membrane ...
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bioRxiv - Microbiology 2024Quote: ... 2 % fetal bovine serum and 8 % chelated fetal bovine serum using Chelex® 100 Resin (BioRad, Ref. 142-1253). To differentiate HaCaT cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Bioengineering 2020Quote: ... The membranes were developed using ECL western blotting substrate (Pierce Biotechnology, Rockford, IL) and scanned by ChemiDoc XRS+ system (BioRad, Hercules, CA). Western blots were quantified using Image Lab 6.0.1 software (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: Luminex profiles utilized the Human Inflammation Panel 1 37-plex assay kit (Bio-Rad) per the manufacturer’s protocol using the laboratory multianalyte profiling system (MAGPIX ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies used for SBA: Alexa 488 labeled mouse anti-human CD9 antibody (MCA469A488, Biorad), PE-labeled mouse anti human CD63 antibody (12-0639-42 ...
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bioRxiv - Cell Biology 2022Quote: ... along with Rhodamine-conjugated ɑ-GAPDH human Fab fragment (1:1000, Bio-Rad, 12004168). The blots were imaged on the ChemiDoc MP system and quantified using Image Lab software (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... and chemokines dosage by Luminex using the 48-Plex pan-human cytokine kit (BioRad) according to the manufacturer procedure ...
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bioRxiv - Cell Biology 2022Quote: ... A FAM-labeled probe was used to target human STMN2 gene (dHsaCNS772300147, Bio-Rad) and a Hex-labeled probe was used to target mouse ApoB (dMmuCNS407594696 ...
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bioRxiv - Cancer Biology 2023Quote: ... media (for mouse or human tissue respectively) and 50 μL Alamar Blue (BioRad, BUF012B), with a corresponding media only control well ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti Human Protein Gene Product 9.5 (PGP9.5) (1:500, MCA4750GA mouse monoclonal, Biorad) for 36 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... for detecting mLIF or Bio-Plex Pro Human Cytokine LIF Set (Bio-Rad, 171B6011M) for detecting hLIF ...
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bioRxiv - Cancer Biology 2021Quote: ... whole cell lysates were separated on 8% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (BioRad). The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: E18.5 cortical protein samples were separated on 8% SDS-PAGE gels for 1hr at 120V and transferred overnight at 20V on to PVDF membranes (BioRad). Blots were probed with rabbit anti-tubulin ß3 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The dried pellets enriched for histones were dissolved in sample buffer (8 M urea, 5% β-mercaptoethanol and 10 mM Tris-HCl (pH 7.0)) and added sample loading buffer (Biorad XT sample buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS lysates from beads after co-IP were resolved on 8% SDS polyacrylamide gels and transferred to nitrocellulose membranes (BioRad). Membranes were blocked with 5% low-fat milk in TBS buffer supplemented with 0.1% Tween 20 (blocking buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA template (up to 8 μL) and nuclease-free water were loaded into a disposable droplet generator cartridge (Bio-Rad). After adding 70 μL of droplet generation oil (Bio-Rad) ...
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Transmembrane Signaling Regulates the Remodeling of the Actin Cytoskeleton: Roles of PKC and AdducinbioRxiv - Cell Biology 2021Quote: ... RT/PCR products were analyzed by 8% polyacrylamide gel and relative band intensity was quantified using Image Lab (Bio-Rad). Most of the primer sequences used were selected from the FlyPrimerBank (Hu et al. ...
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bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was done in triplicate in a total reaction volume of 10-μl containing 0.5-μl of 1:8 diluted cDNA (validated dilution with dynamic range of amplification) and 5nM primer mix using the CFX384 QPCR machine (Biorad). The list of primers used are listed in Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein were separated via 8% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Bio-Rad, Cat no. #1620177) membrane using transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Samples taken from individual fractions were analysed on denaturing SDS polyacrylamide gels (8 %) stained with Coomassie Brilliant Blue (Bio-Rad). Fractions containing pure (>90 % ...
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bioRxiv - Microbiology 2020Quote: ... brucei were separated by SDS-PAGE (8% or 10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) (48) ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed and then precipitated in 0.1 M glycine pH 3.5 and neutralized with 0.5 M Tris?Cl and 1.5 M NaCl pH 7.4 before SDS-PAGE electrophoresis (8%, at 100 V for 90 min) followed by transfer to a PVDF membrane (BioRad). Membranes were washed in Tris-buffered saline (TBS ...
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bioRxiv - Bioengineering 2020Quote: ... GEMs were transferred to PCR 8-tube strips and GEM-reverse transcription was performed in a C1000 Touch Thermal Cycler (BioRad): 53 °C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were separated on an 8% polyacrylamide gel stained with SYBR Gold (Thermo Fischer Scientific) and revealed using a ChemiDoc MP Imaging System (Biorad). Band intensity was quantified using ImageJ package (https://imagej.nih.gov/ij/) ...
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bioRxiv - Developmental Biology 2020Quote: ... Western blots were performed either with Criterion ™ XT Tris-Acetate Precast Gels 3–8 % (3450130, Bio-Rad, Hercules, CA), XT Tricine running buffer (161–0790 ...
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bioRxiv - Microbiology 2020Quote: ... Maltose-1-phosphate was separated from orthophosphate by anion exchange chromatography using Dowex 1 × 8 100-200 mesh (Bio-Rad, 28 x 1.6 cm ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Samples were run on 8-12% SDS-PAGE gel and transferred to a nitrocellulose membrane using a Trans-Blot Turbo Transfer System (BioRad). Membrane was blocked in 5% milk in TBS + 0.1% Tween blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... 4-8 µL of samples and 3 µL of Precision Plus Protein Dual Color Standard (cat. no. 1610374, Bio-Rad) were run on 4-12% Bis-Tris Bolt gels (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... protein lysates were loaded onto 8% SDS-PAGE gel and transferred to a PVDF membrane using a semi-dry transfer system (Biorad) for 10 min at 2.5 amperes ...
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bioRxiv - Cell Biology 2022Quote: ... 10-30 μL of the supernatants (based on protein abundances) were run on an 8% SDS-PAGE gel and transferred to a nitro-cellulose membrane (Cat. No. 1620112, BioRad) using a semi-dry transfer method (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... equal amounts of protein (2.5 or 10 µg) were separated by SDS-PAGE on 4-12% Bis-Tris or 3-8% Tris acetate gels (Criterion XT, BioRad) and transferred to nitrocellulose using TransBlot Turbo (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... All steps were done manually using 8-strip PCR tubes and PCR reactions were carried out on a 96-plate thermal cycler (Biorad). Libraries were pooled and sequenced on a Nextseq550 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate and dilutions were denatured in 1x Laemmli buffer at 95C for 30 seconds and resolved by SDS-PAGE on a 8-20% gradient gel (BioRad). Following trimming of the gel ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were boiled for 8 min at 95 °C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747), resolved on 10% polyacrylamide gels or pre-cast 4-12% Bis-Tris gradient gels (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... were resolved on SDS 8-15% polyacrylamide gels and transferred onto nitrocellulose membranes using the Trans-Blot turbo transfer system (BioRad). Membranes were blotted with the primary antibodies against ADMA (Epicypher ...
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Effects of menopause and high fat diet on metabolic outcomes in a mouse model of Alzheimer’s diseasebioRxiv - Neuroscience 2023Quote: ... Diabetes-associated markers were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (171F7001M; Bio-Rad, Carlsbad, California) according to the manufacturer’s instructions.