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Citations for Bio-Rad :
251 - 300 of 335 citations for Dioctanoyl phosphatidic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... or self-made gel and subjected to a sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) using a Mini-PROTEAN® Electrophoresis cell (Biorad, Cat No 1658002EDU). Gels were run at 130 V for approx ...
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bioRxiv - Microbiology 2022Quote: ... Resulting lipopolysaccharides were separated using gradient 4–20% sodium dodecyl sulfate□polyacrylamide gel (4–20% Mini□PROTEAN® TGX™ Precast Protein Gel, BioRad, Hercules, USA) electrophoresis (SDS□PAGE ...
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bioRxiv - Cancer Biology 2022Quote: Equal amounts of cell lysate were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blocking was performed for 60 min with 5% nonfat dry milk in Tris-buffered saline and Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of cell lysate or concentrated conditioned medium were resolved by sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membrane was blocked for 60min with 5% non-fat dry milk in Tris-buffered saline and Tween 20 (1:1000) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nuclear and total protein samples were separated on sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) electrophoresis and subsequently transferred to polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA). After blocking using 5% of bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad Mini-PROTEAN® TGX™ 4-15% Gel) according to manufacturer’s protocols for electrophoresis at 110 V for 70 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis before being electroblotted onto polyvinylidene fluoride membranes (#1704156, Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with the following primary antibodies for 60 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein were resolved by sodium dodecyl sulfate-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA, USA). Blots were probed with the indicated primary antibodies listed in Extended Data Table 4 ...
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bioRxiv - Microbiology 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Equal protein amounts of the different samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose or polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Feldkirchen, Germany) by semidry blotting ...
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bioRxiv - Genetics 2023Quote: ... The purity of viral preparations was determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis on a 4– 15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Hercules, CA, USA) in Tris-glycine- SDS buffer (Figure S3).
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bioRxiv - Cell Biology 2022Quote: ... 30 μl were loaded and resolved on 6% sodium dodecyl sulfate (SDS) gels and transferred onto nitrocellulose membranes with Mini-PROTEAN III and Mini Trans-Blot cells (Bio-Rad, Hercules, CA, USA), respectively ...
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bioRxiv - Neuroscience 2022Quote: Bilateral tissue punches were homogenized in 1% sodium dodecyl sulfate (SDS) processing buffer and protein was quantified using the Bio-Rad protein DC assay (Bio-Rad Laboratories, Berkeley, CA). For each sample ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of each lysate sample were separated though sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Criterion precast gels (Bio-Rad Cat #456-1095). Proteins were blotted onto Polyvinylidene difluoride (PVDF ...
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The intersection of endocrine signaling and neuroimmune communication regulates neonatal nociceptionbioRxiv - Neuroscience 2024Quote: ... 20 µl of the mixtures were resolved along with Chameleon® Duo Pre-stained Protein Ladder (Lot #928-60001) in 10% precast Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE: Bio-Rad, cat# 456-1030). Samples were separated at 50 V for 30 minutes followed by 100 V for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... equal protein concentrations were loaded onto and separated in 12% (wt/vol) sodium dodecyl sulfate polyacrylamide gel (40% acrylamide/bisacrylamide solution; Bio-Rad, Hercules, CA; 161 0148). Proteins were transferred from the gel to 0.45 m pore size μ nitrocellulose membrane (Maine Manufacturing ...
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bioRxiv - Immunology 2020Quote: ... Protein profile was analyzed using 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described by Laemmli [11] and stained with Coomassie Brilliant Blue (Bio-Rad, Rockville Centre, NY 11571) [12] ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4-20% gradient gels (Bio-Rad #4561095; Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... Equal amounts of protein samples (35 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Physiology 2024Quote: ... Equal amounts of protein samples (40 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electro-transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked with 5% non-fat milk dissolved in Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated in 10% or 12% sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad Life Science, Mississauga, ON). Blots were incubated overnight at 4°C in blocking buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... were separated under reducing conditions by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (4-20% Criterion TGX Stain-free gels, Bio-Rad Laboratories, Hercules, CA, USA) and transferred to Polyvinylidene fluoride (PVDF ...
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of protein were subjected to 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (cat. no. 1620112; Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked using Intercept® (TBS ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was stopped with 1N sulfuric acid and absorbance was measured at 450 nm in the iMarkTM Microplate Absorbance Reader (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
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bioRxiv - Physiology 2020Quote: ... Samples and standards were incubated with bicinchonic acid for 30 minutes prior to measuring absorbance as 540 nm using an iMark microplate absorbance reader (BioRad).
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bioRxiv - Biochemistry 2022Quote: ... Gels were Coomassie stained and destained with 10% acetic acid prior to imaging on a ChemiDoc MP imaging system (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed on a 4-15% polyacrylamide NuPage Bis-Tris gel (invitrogen) in 1x morpholineethanesulfonic acid (MES) SDS running buffer (invitrogen) or on a 4-20% polyacrylamide Criterion gels (BioRad) in 1x Tris/glycine/SDS buffer (BioRad ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
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bioRxiv - Microbiology 2022Quote: ... A Bio-Rad Aminex HPX-87H HPLC column for organic acid analysis (300 × 7.8 mm; Bio-Rad Hercules, CA, USA) was equilibrated with 5 mM H2SO4 (Titrisol ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was resolved by electrophoresis on a 6% non-denaturing polyacrylamide gel in TBE (100 mM Tris, 90 mM boric acid, 1 mM EDTA) buffer and visualised by ChemiDoc (BioRad) using SYBR Gold stain or imaging in the 6FAM ...
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bioRxiv - Developmental Biology 2024Quote: ... The reacted proteins were detected by incubating the membranes in the chemiluminescence solution (luminol, coumaric acid, hydrogen peroxide, Tris pH 6.8) and imaged by the ChemiDoc Gel Imaging System (BioRad, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... which binds non-selectively to nucleic acids and was imaged by the ChemiDoc Imaging System from Bio-Rad (California, USA). The bands were subsequently quantified using ImageJ software ...
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bioRxiv - Cancer Biology 2020Quote: ... an equal amount of proteins was electrophoresed on 8 to 15% sodium dodecyl sulfate-polyacrylamide gels prepared in-house and transferred to polyvinylidene difluoride (PVDF) (Bio-Rad, Hercules, CA, USA; Cat# 1620177) membrane at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins (20-30 μg) of the tissue protein samples were subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad mini-PROTEAN TGX Gels, # 4561044) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped using glacial acetic acid and the N-glycans desalted sequentially using strong cation exchange (AG 50W X8, Bio-Rad), C18 and porous graphitised carbon (PGC ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were then stained with Coomassie Blue solution, followed by de-staining (Methanol, Acetic Acid solution) and visualised by using Gel Doc™ XR+ system (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was stopped by 10% acetic acid and the gel was washed with water for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Antibiotic disk content used in the assays were cefotaxime 30 μg and amoxicillin + clavulanic acid 30 μg (20 μg + 10 μg; all from Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... Reaction was developed using a freshly prepared TMB substrate solution and stopped with 1M Sulfuric acid before determining the OD450 using a microtiter plate reader (iMark mic, Bio-Rad).