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Citations for Bio-Rad :
101 - 150 of 335 citations for Dioctanoyl phosphatidic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Protein concentrations were measured after sonication by the bicinchoninic acid assay (Bio-Rad, Hercules, CA), and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343 ...
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bioRxiv - Systems Biology 2024Quote: Metabolites such as sugars and acids (e.g. lactate and acetate) were measured by Bio-Rad HPX-87H column at 65 °C with 5 mM sulfuric acid as a mobile phase using a Thermo high-performance liquid chromatography (Ultimate 3000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was collected for quantification using the bicinchoninic acid protein kit (Bio-Rad, USA).
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bioRxiv - Microbiology 2020Quote: ... Two microgram of concentrated viruses were resolved on 4-20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (Biorad) using the Novex™ Sharp Pre-stained Protein Standard (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Lyophilized material was resuspended in a special reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer [37] and run on AnyKD gels (Biorad) for 10 min at 120 V ...
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bioRxiv - Biophysics 2020Quote: ... followed by gel filtration in 50 mM sodium citrate buffer (pH 5.5) and 150 mM sodium chloride using an ENrich SEC 650 size-exclusion chromatography column (Bio-Rad). Purified samples were concentrated using Amicon Ultracel-10K centrifugal filter units (EMD Millipore).
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bioRxiv - Cancer Biology 2021Quote: ... whole cell lysates were separated on 8% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (BioRad). The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were denatured and proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on Mini-Protean 10% tris-glycine gels (BioRad). Precision plus protein Kaleidoscope molecular weight (MW ...
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bioRxiv - Biochemistry 2020Quote: ... by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany). Proteins were transferred to a nitrocellulose membrane (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl of sample containing 0.5-1 µg of Benzonase-digested DNA in 25 mM sodium phosphate buffer (pH 6.5) was applied to a nitrocellulose membrane (Bio-Rad) pre-wet in the same buffer using a vacuum slot blot manifold (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Mini-PROTEAN® electrophoresis unit (BioRad, Hercules, CA) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Biophysics 2021Quote: ... were pooled and buffer exchanged to remove excess imidazole (20mM sodium phosphate, pH 7.5, 100mM NaCl, 10% glycerol) using a 10-DG column (Bio-Rad; pre-equilibrated in elution buffer without imidazole and DTT) ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... and then loaded without prior heating on a 4-20% gradient polyacrylamide gel and separated by denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (BioRad). The gel was exposed to a PhosphorImager screen in a cassette containing an enhancing screen ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred with PVDF membrane (Trans Blot Turbo, Cat.#1704157, Biorad). Membranes are blocked in %5 Milk with Tris-buffered saline with 0.1% Tween 20 detergent (TBST) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded in precast 4-20% gradient sodium dodecyl sulfate (SDS) polyacrylamide gels (Mini-PROTEAN TGX gels, Bio-Rad) and run at room temperature with 100 V (constant ...
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bioRxiv - Molecular Biology 2021Quote: ... precast protein gels in sodium dodecyl sulfate (SDS) buffer followed by electrophoretic transfer of proteins to nitrocellulose membrane (BioRad, #2895). After blocking in 5% nonfat dried milk diluted in tris-buffered saline and 1% tween 20 (TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Pathology 2023Quote: ... Equal amounts of protein extract (50 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Bio-Rad, CA, USA) and transferred electrophoretically to nitrocellulose membranes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10% (w/v) Tween 20 and 20% (v/v) sodium dodecyl sulfate (SDS) were purchased from Biorad (Richmond, CA, USA). Absolute ethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride membrane (Cat# 1620177, BioRad). Following the protein transfer ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated eMAP hydrogel monomer solution (30% acrylamide [A9099, MilliporeSigma, St. Louis, MO, USA], 10% sodium acrylate [408220, MilliporeSigma], 0.1% bis-acrylamide [161-0142, Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were electrophoresed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis using a 4%–20% gradient polyacrylamide gel (Bio-Rad, 5678093) and transferred to 0.45-μm polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... and exchanged into 100 mM sodium phosphate buffer (pH 7.0) supplemented with 100 mM sodium chloride using Econo-Pac 10DG desalting pre-packed gravity-flow columns (Bio-Rad). Proteins used for crystallization were further purified by gel filtration in 50 mM sodium citrate buffer (pH 5.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins used for crystallization were further purified by gel filtration in 50 mM sodium citrate buffer (pH 5.5) and 150 mM sodium chloride using an ENrich SEC 650 size-exclusion chromatography column (Bio-Rad). Purified samples were concentrated using Amicon Ultracel-3K centrifugal filter units (EMD Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 μg protein extracts were separated by sodium dodecyl sulfate (SDS) gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad) overnight ...
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bioRxiv - Biophysics 2022Quote: ... washes and elutes were examined using SDS-PAGE (sodium dodecyl sulfate– polyacrylamide gel electrophoresis) (Gel: BioRad miniPROTEAN TGX AnyKD - 4569036; MW latter: BioRad Precession Plus Protein Standard Unstained – 1610363) ...
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bioRxiv - Genetics 2023Quote: ... The beads were washed four times for 5 min with RIP buffer containing 0.5% sodium deoxycholate and boiled in 1xLaemmli Sample Buffer (Bio-Rad) for 10 min at 100°C ...
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bioRxiv - Cancer Biology 2023Quote: ... separated by sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% of bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... onto 4-15% precast criterion sodium dodecyl sulfate polyacrylamide gel electrophoresis gradient gels and transferred to PVDF per manufacturer’s protocol (Bio-Rad). The protein sizing ladder used was Precision Plus Protein Standards (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 µg of protein was separated on a 4–20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Neuroscience 2024Quote: ... were electrophoresed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and transferred proteins from gel onto polyvinylidene difluoride fluoride (PVDF) membranes (BioRad). After transformation ...
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bioRxiv - Developmental Biology 2024Quote: Prepared samples were loaded onto a 7.5% (Elp1) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (Biorad 4561033, 4561024 respectively) and separated by electrophoresis at 70V for approximately 2.5 hours in 1X running buffer (diluted from 10X running buffer (25mM Tris base ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled in SDS loading buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to 0.45 µm nitrocellulose membranes (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2020Quote: ... lysate concentrations were adjusted to equal protein concentrations using the bicinchoninic acid (BCA) protein assay (Biorad). Equal amounts of protein were mixed to a final concentration of 1x reducing sample buffer (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... boric acid) Urea solutions were made fresh daily and incubated with AG 501-X8 resin (Biorad) for minimum 5 hours to remove ionic contaminants ...
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bioRxiv - Microbiology 2022Quote: ... An Aminex HPX-87H Organic Acid Analysis Column (300 × 7.8 mm; Bio-Rad, Hercules, CA, USA) was equilibrated with 5 mM H2SO4 (Titrisol ...
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bioRxiv - Cancer Biology 2023Quote: Nucleic acids (RNA or DNA) samples were loaded in Bio-Dot SF Microfiltration Apparatus (Bio-Rad) and allowed to bind onto the membrane ...
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bioRxiv - Immunology 2024Quote: ... 10mL of 10% (v/v) acetic acid was incubated in poly-prep chromatography columns (Bio-Rad) for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of purified proteins was determined using the Bradford assay and purity was verified by electrophoresis in sodium dodecyl sulfate (SDS)-polyacrylamide gel which was subsequently stained using Bio-Safe Coomassie G 250 (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... twice in 200 μl nuclease free water and once in 100μl 1x SSC (150 mM NaCl, 15mM sodium citrate, pH 7.0, BIO-RAD Laboratories, Inc.) and finally resuspended in 50 μl 1X SSC ...
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bioRxiv - Biochemistry 2020Quote: Cell lysates were heated at 95°C for 5 min in Laemmli Sample Buffer and then subjected to sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE; (52)) followed by electrophoretic transfer to 0.45 μm nitrocellulose membranes (Bio-Rad; 1620115). Membranes were blocked in PBS-0.05% Tween20 (PBST ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amount of protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Criterion TGX Stain-Free Precast Gels (Bio-Rad) and transferred onto Immobilon-P membranes (MilliporeSigma) ...
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bioRxiv - Microbiology 2020Quote: ... or the eluates were separated on a 4-15% gradient sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pre-casted gel (Bio-Rad). For Mn2+-Phos-tag SDS-PAGE gel ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were collected then Gag was immunoprecipitated using an α-RSV antibody and were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and quantified using a PhosphorImager (Bio-Rad). After 35S-Met/Cys labeling for 2.5-hours ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated on a polyacrylamide gel with 10% of sodium dodecyl sulfate(SDS) (Mini Protean III; Bio-Rad, Richmond, CA), and they were transferred for 2 hours at 48°C to nitrocellulose membranes of 0.45 μm ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were resolved by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) using 4-20% precast gel (Biorad, Hercules, CA). Proteins were transferred by semi-dry transfer onto a PVDF (polyvinylidene fluoride ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins (20 μg equivalent) were then separated by 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; Bio-Rad), then transferred by using 0.22-μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Macrosep and Microsep Advance centrifugal devices 3 kDa MWCO and 0.2 μm syringe filters were purchased from Pall Corporation ...
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bioRxiv - Microbiology 2021Quote: ... or wet transferred using 0.5 M sodium phosphate transfer buffer (at a constant 1 Amp for an hour using a Bio-Rad apparatus). Membranes were blocked in 5% milk in PBS-T and probed with primary antibody (overnight at 4°C ...