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Citations for Bio-Rad :
251 - 300 of 328 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Protein concentrations were determined by use of the Bradford assay (Bio-Rad protein assay kit II, Cat. No. 5000002). For immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μg of in vitro-transcribed RNA (2.5 μg of SNX27/LRFN2 RNA and 2.5 μg of the defective helper plasmid) was electroporated into 0.6×107 BHK-21 cells using a Gene Pulser II electroporation system (BioRad) in a gene pulser cuvette (0.2 cm gap) ...
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bioRxiv - Biochemistry 2021Quote: ... Transcriptionally active Q0.3 fraction (0.32–0.4 M) were pooled and applied directly to hydroxyapatite (HAP) type II ceramic resin (Bio-Rad), washed first at 0.38 M ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were then separated on 12 % polyacrylamide gels (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France) and transferred to 0.2 μm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The virus genome solutions of each well were homogeneized by shaking and two 2 μL aliquots were taken for qPCR titration using the my-Budget 5x EvaGreen qPCR-Mix II (Bio-Budget) following the manufacturer’s instructions and a CFX96 Real-Time System machine (BioRad). See supplementary table 1 for primers.
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bioRxiv - Physiology 2024Quote: ... The total protein content was determined using the colorimetric Bio-Rad Protein Assay Kit II (Bio-Rad Laboratories, CA). Seven dilutions of a protein standard containing 0 to 30 µg/mL of the total protein content were used ...
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bioRxiv - Genomics 2023Quote: ... to ensure high integrity and later analyzed with pulse-field gel electrophoresis on CHEF DR-II (BioRad, CA, USA) to ensure the size range between 20 kb to 100 kb ...
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bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined by a detergent compatible DC™ Protein Assay Kit II (Bio-Rad Laboratories, #5000112). For Western blot analysis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supernatant was recover and protein concentration was measured using the DC™ Protein Assay Kit II (Bio-Rad, 5000112). Lysates were boiled in the NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Neuroscience 2024Quote: ... Counts per minute (CPM) were quantified and normalised to overall protein using a DC protein assay kit II (Biorad).
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bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... Electroporation was performed at 0.31 kV and 950 μF with maximal capacitance using a Gene Pulser II system (Bio-Rad). The parasites were then cultured in complete medium at 1% hematocrit at 37 °C ...
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bioRxiv - Immunology 2020Quote: The VSGm protein samples were separated on 10% SDS-PAGE then trans-blotted onto two separate nitrocellulose membranes using the mini PROTEAN II Trans-blot unit (Biorad). The membranes were rinsed in 1x PBS (0.1% w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosomal DNA was separated in 1% agarose/0.5 × TBE on the CHEF-DR II Pulsed Field Electrophoresis Systems (Bio-Rad) as follows ...
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bioRxiv - Molecular Biology 2020Quote: Yeast genomic DNA was prepared in an agarose plug as described in the Instruction Manual (Bio-Rad, CHEF-DR II) with slight modifications ...
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bioRxiv - Plant Biology 2023Quote: ... Linearized plasmid was mixed with 250 μL of cells at 15°C in a 0.4 cm gap electroporation cuvette and transformed immediately by electroporation using a Gene Pulser II (Bio-Rad) set to 800V and 25 μF ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-FLAG-HRP (clone D6W5B, CellSignalingTechnology #86861S, RRID:AB_2800094) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P, RRID:AB_609796) detection Abs were added for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasma vitamin B12 concentrations were assessed using the BioRad Quantaphase II radio assay (Hercules, CA, USA;74. Plasma folate concentrations were also measured using the BioRad Quantaphase II radio assay (Hercules ...
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bioRxiv - Plant Biology 2023Quote: ... A quantitative real-time PCR (RT-qPCR) assay was performed on a LightCycler 480 II system using 2x SYBR Green qPCR master mix (BioRad). Maize GAPDH (Zm00001eb233140 ...
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bioRxiv - Immunology 2024Quote: The concentration of total protein in each processed sample was quantified using the DC Protein Assay Kit II (Bio-Rad) with a standard curve (R2 > 0.99 ...
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bioRxiv - Physiology 2024Quote: ... Real-time qPCR was performed using SYBR Premix ExTaq II in a CFX96 Real-Time qPCR Detection System (Bio-Rad). Glyceraldehyde 3-phosphate dehydrogenase (Gapdh ...
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bioRxiv - Neuroscience 2024Quote: ... the mouse was returned to its home cage and the paper was imaged by a gel imaging system (ChemiDocTM MP, Universal Hood II, BioRad) with the Krypton detection setting ...
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
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bioRxiv - Microbiology 2020Quote: ... We covered the wells with a coverslip and we let them polymerize in a UV transilluminator (Bio-Rad Universal Hood II) for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The mixture was subjected to two consecutive electrical pulses of 850 V at 20 μF with a Gene Pulser II electroporator (Bio-Rad) and the cells were then seeded on a confluent monolayer of LR7 feeder cells in a 35mm dish ...
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bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
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bioRxiv - Developmental Biology 2021Quote: ... and 25 µg of the targeting vector was transfected into 1 × 107 of Nmt1-heterozygous cells11 by electroporation (240 V, 500 µF) with Gene Pulser II (Bio-Rad). One week later ...
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bioRxiv - Microbiology 2020Quote: ... baumannii cells using a Bio-Rad GenePulser II (1.8 kV, 25 μF, and 200 Ω) in a 1 mm electrode gap cuvette (Bio-Rad). Cells were recovered by resuspension in 1 mL of SOC medium and incubated at 37°C with shaking (200 rpm ...
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bioRxiv - Zoology 2020Quote: ... Telomeric repeats of sequence TTAGG were separated from restricted DNA with Pulsed Field Gel Electrophoresis (CHEF-DR II system by Bio-Rad) in a 0.8% agarose gel.
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with cold water and 10% glycerol and electroporated with the cat or kan PCR product using a GenePulser II instrument (2.5 kV, 0.2 cm gap cuvettes; Bio-Rad, Hercules, CA). Transformants were verified by colony PCR with primers approximately 200 bp up- and downstream of the gene of interest ...
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bioRxiv - Microbiology 2020Quote: ... Samples were assayed by Taqman multiplex real-time PCR using TaqMan Universal Master Mix II and a CFX384 Touch™ Real Time PCR detection system (BioRad). A standard curve was obtained using an in vitro transcript derived from a synthetic plasmid that contained all PCR targets ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Genomics 2024Quote: ... and chromosomal separation was conducted with the CHEF DR-II System and the CHEF Mapper XA System (Bio-Rad, Richmond CA) using different running and buffer conditions ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reactions were analysed by agarose gel electrophoresis and image capture was performed on a Universal Hood II Gel Doc System (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: Hydrazine synthase α-subunit was purified by applying the 200 mM NaCl fraction from the Q-sepharose column on a 50 ml hydroxyapatite type II 40 μm (Bio-rad) column (XK 26/20 ...
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bioRxiv - Microbiology 2023Quote: ... The suspension was electroporated (310 V, 950 µF, 200 Ω, exponential) in a 2mm electroporation cuvette with a GenePulser II (BioRad). Electroporated parasites were immediately transferred into 5 mL complete culture containing 100 µL fresh ...
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... and 10 µL of this suspension was loaded into a well of a 15-well polyacrylamide gel of the Mini-PROTEAN II electrophoresis apparatus (Bio-Rad). The percentage of polyacrylamide was 10% for the separating gel and 4% for the stacking gel.
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bioRxiv - Cancer Biology 2024Quote: ... The resulting supernatants were retained and protein concentrations were determined using the DC Protein Assay Kit II (Bio-Rad, Feldkirchen, Germany).
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bioRxiv - Plant Biology 2020Quote: Forward and reverse new PCR primers for the 12 Zn transport-related genes and the two reference genes suitable for SYBR® Green II RT-qPCR assays (Biorad, USA) were designed (Table 2) ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...
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bioRxiv - Genetics 2020Quote: ... agarose gel that was then stained with SYBR Green II (Tiandz, China) and imaged using a UV transilluminator from Syngene (Bio-Rad, USA).
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bioRxiv - Biophysics 2022Quote: ... HbA (from transfusion)) was analyzed via High Performance Liquid Chromatography with a Bio-Rad Variant II Instrument (Bio-Rad, Montreal, QC, Canada) in the core clinical laboratory of University Hospitals Cleveland Medical Center (UHCMC) ...
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bioRxiv - Neuroscience 2023Quote: Proteins were separated on SDS-PAGE under denaturing conditions and transferred to nitrocellulose membrane using a MINI PROTEAN II™ system (Bio-Rad). Membranes were blocked with 5% milk powder/TBS-T and incubated with the primary antibodies overnight at 4 °C ...