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Citations for Bio-Rad :
51 - 100 of 328 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and CyaY were buffer exchanged into 200 mM ammonium acetate (pH = 7.5) using Micro Bio-Spin 6 Columns (Bio-Rad). Instrument parameters were tuned to maximize transmission but simultaneously preserve the native-like state of proteins as determined by ion mobility ...
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bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: All proteins except for the full-length HSPB7 were buffer-exchanged into 200 mM ammonium acetate (pH 6.9) using biospin (Bio-Rad); full-length HSPB7 was buffer-exchanged into 1 M ammonium acetate (pH 6.9) ...
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
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bioRxiv - Biochemistry 2024Quote: AcrAS was buffer exchanged into a volatile solution (100 mM ammonium acetate, pH 6.0) using a centrifugal exchange device (Micro Bio-Spin 6, Bio-Rad) according to manufacturer’s instructions native MS experiments were performed on a Synapt G2-Si mass spectrometer (Waters) ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 mM ammonium acetate at the desired pH using a centrifugal buffer exchange device (Micro Bio-Spin 6, Bio-Rad). Stock solutions of lipids were prepared from chloroform/methanol solution by evaporating aliquots of known volume in a SpeedVac ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02) on Bio-Rad CFX-96 Touch Real-time PCR System (Bio-Rad, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Immunology 2024Quote: ... was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02) on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad). The primers used can be found in Table 1 and Ct values were normalized to an internal control (Gapdh and β-actin).
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bioRxiv - Cell Biology 2024Quote: ... qPCR was conducted with ChamQ SYBR Color qPCR Master Mix (Q431-02, Vazyme) on a CFX Connect 96-well system (Bio-Rad, USA). Target gene expression levels were quantified using the 2−ΔΔCt method ...
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bioRxiv - Biochemistry 2021Quote: ... followed by hydroxyapatite (CHT-II, Bio-Rad), and finished with SEC (Superose 6 Increase ...
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bioRxiv - Cancer Biology 2023Quote: ... The DC protein assay kit II (BioRad) was used to quantify protein concentrations ...
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bioRxiv - Microbiology 2023Quote: ... and Foresight CHT Type II (Bio-Rad) columns with sodium phosphate gradient ...
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bioRxiv - Cell Biology 2023Quote: ... using a Gene Pulser II (Bio-Rad) set at 1.8 kV ...
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bioRxiv - Microbiology 2024Quote: ... 25 μF (Bio-rad Gene Pulser II) using a 1 mm gap cuvette (VWR #732-1135) ...
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bioRxiv - Biochemistry 2024Quote: ... ii) Mini-PROTEAN Tetra Cell (Bio-Rad), in combination with Mini-PROTEAN TGX precast protein gels (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... The upper phase was collected and RNA extracted using the Aurum total RNA kit (Bio-Rad) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2021Quote: Polymerizing gel solution was made by combining 15 mL of 9.5% gel solution with 133 uL of 10% Ammonium Persulfate Solution (APS) and 13.3uL TEMED (BIO-RAD #161-0700) into a beaker under a fume hood ...
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bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: Protein samples were buffer exchanged into 200mM ammonium acetate using either a centrifugal buffer exchange device (Micro Bio-Spin P-6 Gel, Bio-Rad) or a dialysis device (Slide-A-Lyzer MINI Dialysis Unit ...
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bioRxiv - Synthetic Biology 2022Quote: ... 0.02% Bromophenol blue) without a subsequent boiling step and loaded (13ul) using commercially available precast 12% SDS-PAGE gels (BioRad, Singapore; Cat: 4561046). Gels were illuminated with a blue light transilluminator ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Microbiology 2021Quote: ... protein pellets were resuspended in 100 µL of 6M urea in 50mM ammonium bicarbonate for DC® protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Microbiology 2021Quote: ... 25 mL of log-phase Sodalis cells (OD600= 0.3∼0.5; SmartSpec Plus spectrophotometer; Bio-Rad, Hercules, CA) were washed consecutively in 25 mL ...
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bioRxiv - Genomics 2020Quote: ... using a Gene Pulser II device (Bio-Rad). Transformed cells were recovered in S.O.C media (from ElectroMAX Stabl4 kit ...
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bioRxiv - Plant Biology 2021Quote: ... using Gene Pulser II (Bio-Rad, Hercules, CA) with a 0.2 cm-gap cuvette at 2.5 kV cm-1 ...
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bioRxiv - Cell Biology 2022Quote: ... using a Gene Pulser II (Bio-Rad Laboratories) according to manufacturer’s instructions using the following electroporation conditions ...
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bioRxiv - Genetics 2024Quote: ... using the DC Protein Assay Kit II (BioRad). Luciferase activities were measured on a GloMax Luminometer (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... using a Gene Pulser II electroporator (Bio-Rad), 3 µg linearized vector ...
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bioRxiv - Immunology 2024Quote: ... and a Gene Pulser II instrument (Bio-Rad) with settings of 950 µF and 220V ...
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bioRxiv - Microbiology 2024Quote: ... The Gene Pulser® II electroporator (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: Agrobacterium tumefaciens (strain GV3101::mp90) was electroporated in the presence of 500 ng binary vector (pGreen II) using a gene pulser II (Biorad, USA) set to 2.5 kV ...
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bioRxiv - Biochemistry 2021Quote: ... and their respective complexes with Hap40 protein were buffer-exchanged into 150 mM aqueous ammonium acetate (pH=7.5) by using P-6 Bio-Spin gel filtration columns (Bio-rad, Veenendaal, the Netherlands). The protein’s resulting concentration was estimated to be ~2-5 μM before native MS analysis ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Biophysics 2023Quote: Proteins samples were kept on ice and buffer exchanged into 200 mM ammonium acetate buffer at pH 7 using Micro Bio-spin columns with either a 6 or 30 kDa cutoff (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM before analysis ...
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bioRxiv - Biochemistry 2024Quote: Protein samples (S100A9ec and S100A9in) were buffer exchanged into 200 mM ammonium acetate at pH 7 using Micro Bio-spin P6 columns (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM ...
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bioRxiv - Microbiology 2024Quote: ... 0.02% bromophenol blue) was added to samples and loaded onto a 12% polyacrylamide Mini-Protean TGX pre-cast gel (Bio-Rad, does not contain SDS). Samples were electrophoresed in 25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... using a Gene Pulser II Electroporation System (Bio-Rad). Then ...
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bioRxiv - Immunology 2021Quote: ... using a GenePulser II with Capacitance Extender (Bio-Rad). Cells were recovered at room temperature for 10 minutes and plated in 6-well culture plates in complete DMEM or RPMI for 24 hours at 37°C ...
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bioRxiv - Pathology 2022Quote: ... using Bio-Rad Protein Assay Kit II (Bio-Rad), or DNA ...
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bioRxiv - Cell Biology 2021Quote: ... using a mini-protean II apparatus (Bio-Rad, UK) at a constant 85V ...
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bioRxiv - Biophysics 2020Quote: ... linearized plasmid using a Gene Pulse II (Bio-Rad). Clone selection was performed as previously described by an in-house two-step transformant screening strategy 48 to maximize the expression of receptor for subsequent fluorescence labeling 49.
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bioRxiv - Cancer Biology 2020Quote: ... using wet transfer Mini-PROTEAN II electrophoresis system (BioRad). Membranes were blocked in 5% skim milk (Fluka ...
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bioRxiv - Biophysics 2021Quote: ... Cuvettes were transferred to a Gene Pulser II (BioRad) and pulsed with the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... Bio-Rad Protein Assay Kit II (Bio-Rad laboratories) was used to measure the acetylated tubulin concentration from the post-nuclear supernatant collected from the lysates ...
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bioRxiv - Genomics 2024Quote: ... DNA was separated using CHEF-DR II (Bio-Rad). The DNA ladder used was the CHEF DNA Size Marker (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... and electroporated with Gene Pulser® II (Bio-Rad) at a voltage of 0.35 kV and capacitance of 25 µF ...
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bioRxiv - Biochemistry 2021Quote: ... Unincorporated nucleotides were removed from the aqueous phase by using a P-30 Microspin column (RNase Free; Bio-Rad), and the oligonucleotide concentration was measured by using a Qubit ssDNA assay kit (Thermo Fisher Scientific) ...