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Citations for Bio-Rad :
251 - 300 of 4372 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Tween-20 (Bio-Rad), and Glycine (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... 0.1% Tween-20 (BioRad) PBS solution for 1 hour at RT ...
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bioRxiv - Immunology 2022Quote: ... using the transfer buffer (192 mM glycine, 25 mM Tris, 20% methanol) for 20 min at 20 V semi-dry transfer apparatus (BioRad, USA). Blocking was done with 1% BSA for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: The 20 μl RT-dPCR reaction mixture consisted of 5 μl One-Step RT-dPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 µl of Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Physiology 2022Quote: ... such that a total of 2 μg protein in 20 μL of buffer was loaded into wells of a Mini-PROTEAN TGX Stain-Free gel (5-14%) (Bio-Rad, Mississauga, ON, Canada). Protein ladder (5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... Proteins were denatured at 94°C for 5 min before separation on SDS-PAGE (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels were used (Bio-Rad Laboratories)) ...
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bioRxiv - Cell Biology 2024Quote: ... and boiled for 5 min before loading into a 12% or 4-20% acrylamide pre-cast SDS- PAGE gels (Bio-Rad, 4561045 or 4568094) for electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... CHAPS (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate) were purchased from Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... samples were buffer exchanged into 150 mM of ammonium acetate pH 8 using Bio-Spin P6 columns (BioRad) and the final protein concentrations were adjust to 1-5 µM ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... unreacted ITC-DTPA was removed by dialysis against 0.25 M ammonium acetate (NH4Ac) pH 5.4–5.5 (metal free) supplemented with 2 g/L Chelex (Bio-Rad) using 20,000 molecular weight cut-off dialysis cassettes (Slide-a-Lyzer ...
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bioRxiv - Biochemistry 2022Quote: ... solutions containing purified CytbX were buffer-exchanged into 0.2 M ammonium acetate (pH 8.0) containing 2x CMC of Lauryldimethylamine oxide (LDAO) using Biospin 6 columns (Biorad). 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1× TBE buffer at 2.0 V/cm for 21 hr at room temperature with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). The gel was stained with 0.5 μg/ml of ethidium bromide.
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
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bioRxiv - Molecular Biology 2024Quote: ... The same AAV2 ITR primers were used for ddPCR along with a FAM labeled probe 5 ’-CACTCCCTCTCTGCGCGCTCG-3 with the QX200 Bio-rad Laboratories’ droplet digital system (Bio-rad Laboratories, California, USA) and ddPCR supermix for probe (Bio-rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... WHO°3 n=3) using the Bio-Plex Cell Lysis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 30μg total protein from tissue samples with 4X Laemmli buffer was heat denatured at 99°C for 5 minutes and ran on resolving SDS PAGE gel (Biorad 4-20% precast gel #4561096). Sperm protein samples were prepared using a low volume of lysis buffer (∼100) ...
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bioRxiv - Cell Biology 2020Quote: ... Neutralized samples were incubated with Safranin-O solution (0.05% in 50 mM sodium acetate) on a dot blot apparatus (BioRad) with a 0.45 μm nitrocellulose membrane in duplicate ...
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bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
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bioRxiv - Microbiology 2022Quote: All proteins were buffer exchanged into 0.2 M ammonium acetate using Bio-Spin P-6 Size Exclu-sion Spin Columns (BioRad) as previously described47 ...
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bioRxiv - Biochemistry 2021Quote: ... Preparation of the 5x Tris Acetate buffer was completed by dissolving 145.4 g Tris base (BIO-RAD, 161-0719) in 700 mL of MilliQ water (pH expected between 11.0 and 11.4) ...
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bioRxiv - Molecular Biology 2023Quote: ... Bands were resolved in precooled Tris-acetate-EDTA (TAE) buffer on a 10 % TGX (Tris-Glycine eXtended)-Gel (BioRad). Gels were running at 4 °C and 100 V (≈ 30–35 mA ...
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bioRxiv - Microbiology 2022Quote: ... Acetate was measured by high performance liquid chromatography (HPLC) with an Aminex HPX-87H column (Bio-Rad, Hercules, CA), coupled to an RI and UV detectors (Waters ...
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bioRxiv - Microbiology 2022Quote: ... Acetate was measured by high performance liquid chromatography (HPLC) with an Aminex HPX-87H column (Bio-Rad, Hercules, CA), coupled to RI and UV detectors (Waters ...
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... 20 µg protein per lane was loaded onto a 4-20% gel (Bio-Rad) and samples were run at 50 mA followed by transfer on PVDF membranes using the Trans-Blot Turbo Transfer System (biorad) ...
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bioRxiv - Genetics 2024Quote: ... Approximately 20-30µg of total protein was separated using 4-20% SDS-PAGE (Biorad), transferred to nitrocellulose (SantaCruz ...
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bioRxiv - Molecular Biology 2021Quote: ... 4–20% gel (Bio-Rad) using SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... 4-20% TGX gels (BioRad) were used ...
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bioRxiv - Cell Biology 2021Quote: ... 4-20% gradient (Bio-Rad,); and then proteins were transferred to PVDF membranes ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20 (Bio-Rad, cat# 1706531] for a minimum of 30 minutes and then incubated overnight in primary antibodies diluted in the milk-TBSt blocking solution ...
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bioRxiv - Biophysics 2024Quote: ... 0.1 % Tween-20 (Bio-Rad) pH 7.6 (TBST ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein was resolved in 4-20% precast polyacrylamide gel (Bio-Rad, USA). For immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg sample were loaded onto 4-20% Mini-PROTEAN® TGXTM gels (Bio-Rad), and SDS-PAGE was performed for 40 min (200 V ...
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bioRxiv - Microbiology 2024Quote: ... using precast 4-20 % polyacrylamide gels (4-20 % CriterionTM TGX BioRadTM, BIO-RAD, Hercules, USA).
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bioRxiv - Genetics 2023Quote: ... 20 μg of protein was loaded in a 4-20% precast protein gel (Biorad, #4561096) and subjected to electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 or 40 µg were loaded onto a 4-20% pre-cast gel (Bio-Rad) alongside with molecular weight markers (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cellular protein (∼20 µg) was separated on a 4-20% polyacrylamide gel (Bio-Rad) by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred onto Immobilon-FL PVDF membrane (Millipore) ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 µL of cell lysates were separated via 4-20%–gradient SDS-PAGE (Bio-Rad) and transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Biochemistry 2020Quote: ... and CyaY were buffer exchanged into 200 mM ammonium acetate (pH = 7.5) using Micro Bio-Spin 6 Columns (Bio-Rad). Instrument parameters were tuned to maximize transmission but simultaneously preserve the native-like state of proteins as determined by ion mobility ...
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bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
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bioRxiv - Biochemistry 2022Quote: ... were neutralized by adding an equal volume of 2 M ammonium acetate (pH 7.0) and vacuum-transferred to a pre-washed nitrocellulose membrane using a BioDot SF microfiltration apparatus (Bio-Rad). Each well was washed 2 times with SSC buffer ...
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bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: All proteins except for the full-length HSPB7 were buffer-exchanged into 200 mM ammonium acetate (pH 6.9) using biospin (Bio-Rad); full-length HSPB7 was buffer-exchanged into 1 M ammonium acetate (pH 6.9) ...
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...