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Citations for Bio-Rad :
201 - 250 of 4372 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
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bioRxiv - Biochemistry 2021Quote: ... and the sample was heated to 90°C for 5 minutes before loading of 3 pmol onto a 4-15% Mini-PROTEAN TGX Stain-Free Precast Gel (Bio-Rad), alongside PageRuler Prestained Protein Ladder (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in TBS-T/milk for 45 min and washed 3 × 5 min with TBS-T before detecting using a Chemidoc imager (Bio-Rad). NearIR-conjugated secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were washed in 1X PBS with 0.1% Tween 3 times for 5 minutes each before scanning using the ChemiDoc™ MP Imaging System (Bio-Rad). Note ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times for 5 min with TBS and were subsequently incubated with Clarity Western ECL substrate working solution (Bio-Rad) for 5 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed 3 times with TBST for 5 min each and then the membrane was incubated with Clarity Western ECL Substrate (Bio-Rad) for 2 min before exposure to film and development in a darkroom.
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bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out with 3-5 μL of the linear PCR product (metH homology extension-flanked kan cassette) at 2.5 kV (Bio-Rad MicroPulser), and cells were immediately recovered with 1 mL SOC and cultured at 37°C for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Biophysics 2023Quote: ... Viroporins were exchanged into each of these ammonium acetate solutions using BioSpin 6 columns (Bio-Rad) and diluted to a final protein concentration of 20 μM (per monomer ...
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bioRxiv - Biochemistry 2024Quote: ... 4-20% (BioRad), or 6% (Thermofisher ...
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bioRxiv - Genetics 2021Quote: ... Forty micrograms of total protein from hippocampal nuclear lysates was boiled for 5-minutes to dissociate complexes before separation in a 4-20% gradient gel (Bio-Rad 4561096) for 1-hour at 150V in 1x Tris/Glycine/SDS (Bio-Rad 1610732) ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked for 1 h at room temperature (RT) in Tris-buffered saline with 0.05 % Tween-20 (TBST) containing 5 % blotting-grade dry milk (Biorad cat# 170-6404), incubated overnight with primary antibody (dilutions above mentioned ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were incubated with TBST-Blotto buffer (50 mM Tris-pH 7.5, 150 mM NaCl, 0.1% Tween-20, 5% w/v Bio-Rad Blotting Grade Blocker) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Five µL of each supernatant were run on a 5% to 20% gradient polyacrylamide gel (Wako) and then transferred onto an Immun-Blot® polyvinylidene fluoride membrane (Bio-Rad). Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween-20 (PBST ...
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bioRxiv - Immunology 2023Quote: ... Blots were probed using primary antibodies in PBST (1X DPBS with 0.05% Tween-20 (BioXtra, catalog #P7949) with 5% Blotting Grade Blocker (Bio-Rad, catalog #1706404) for approximately 16 hours (overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... heated up for 10 minutes at 95°C and centrifuged before loading onto a 10 well 5-20% gradient gel (BioRad 456-1093). The finished gels were blotted via semi dry method (20V ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were boiled at 95 °C for 5 min and separated by a 4–20% Mini-PROTEAN® TGX Stain-Free™ SDS PAGE (BioRad) or 12 % Tris-Glycine SDS PAGA (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then blocked for 1 hour in 5% non-fat milk in PBS containing 0.1% Tween 20 (Bio-Rad, Hercules, CA) at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μg of in vitro-transcribed RNA (2.5 μg of SNX27/LRFN2 RNA and 2.5 μg of the defective helper plasmid) was electroporated into 0.6×107 BHK-21 cells using a Gene Pulser II electroporation system (BioRad) in a gene pulser cuvette (0.2 cm gap) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The primer sequences for detecting ACTB are 5’-GACCTGACTGACTACCTCAT-3’(forward) and 5’-TCTCCTTAATGTCACGCACG-3’ (reverse) Quantitative PCR (qPCR) reactions were conducted using the SYBR green PCR master mix (Bio-Rad, 1725121) in CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were immobilized on methanol equilibrated cellulose acetate membrane through a dot blot void system (BioRad).
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bioRxiv - Microbiology 2020Quote: Extracellular concentrations of organic acids (acetate, lactate, formate) and ethanol (from 2.4.3) were determined by HPLC (BioRad HPX-87H 300*7.8 mm column ...
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bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted 1:10 and 5 μl of the diluted cDNA was loaded in a total volume of 20 μl (SYBR Green Supermix (Bio-Rad 172-5124) and run on the Bio-RAD CFX Connect Real-Time System ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated 5 min at 95°C and separated on 4-20% Mini-PROTEAN® TGX Stain-Free™ Precast gels (Bio-Rad) or on home-made 7% polyacrylamide gel for OPA1 immunodetection ...
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled at 95°C for 5 min and resolved in 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed overnight at 4°C with primary antibodies ...
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bioRxiv - Genetics 2022Quote: ... we made 21 µL of reaction mix for each sample according to the manufacturer’s directions (Bio-Rad Laboratories, Hercules, CA). Each reaction consisted of 2× ddPCR Supermix for Probes ...
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bioRxiv - Cell Biology 2022Quote: ... Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) in exactly 2.4L of 0.5x Tris-borate/EDTA buffer in a Rotaphor apparatus (Biometra ...
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bioRxiv - Microbiology 2022Quote: ... To this end, BHK-21 cells were electroporated (1500V, 25μFd, no shunt resistance) using a Gene pulser apparatus (Bio-Rad) with viral RNA produced by in vitro transcription (RiboMax P1300 ...
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bioRxiv - Bioengineering 2023Quote: ... was electroporated (pulsed twice using a square wave of 850 volts for 25 milliseconds) into BHK-21 cells using a Gene Pulser Xcell electroporator (BioRad); transfected cells were cultured in a T175 for 48h at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 60 min at 37°C and a final 10 min at 21°C in a T100 Thermal Cycler (BioRad). All samples were pooled ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... were detected as dark spots after a 10-min reaction with 5-bromo-4-chloro-3-idolyl phosphate and nitro blue tetrazolium using an alkaline-phosphatase-conjugate substrate (Bio-Rad, CA, USA). SFUs were counted using the AID ELISpot Reader System (Autoimmun Diagnostika) ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Evolutionary Biology 2022Quote: ... 0,5 Tween-20 (Biorad), 0,5 NP-40 (Sigma Aldrich ...