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Citations for Bio-Rad :
251 - 300 of 8842 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Stain free gels (BioRad, 456-8093, 4-20%) were transferred onto low-fluorescence PVDF blots (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Precast 4-15% TGX protein gels (Bio-Rad) were loaded with 10-30 µg of total protein lysate and run for 1h at 110-135V ...
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bioRxiv - Neuroscience 2022Quote: ... or Mini-PROTEAN 4–15% Gels (Bio-Rad) and transferred onto PVDF membrane ...
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bioRxiv - Cell Biology 2024Quote: ... Following 4-20% gradient SDS page gel (Biorad) electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... 4%–15% acrylamide (Bio-Rad, catalog #456 –1086) at 185V for 40 min ...
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bioRxiv - Neuroscience 2024Quote: ... 4–20% Mini-PROTEAN Stain-Free Gels (BioRad) were utilized to study the rest of the targets ...
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Temporal Dynamics of Neocortical Development in Organotypic Mouse Cultures: A Comprehensive AnalysisbioRxiv - Neuroscience 2024Quote: ... 4 µl of iScript reaction mix (Bio-Rad), 1 µl of iScript reverse transcriptase (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... or 4-15% Tris-glycine gels (Bio-Rad). After sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Immunology 2024Quote: ... separated on 4-20% polyacrylamide gels (Bio-Rad), and transferred onto PVDF membrane (Millipore) ...
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bioRxiv - Developmental Biology 2022Quote: ... with 4% donkey serum (Bio-Rad Laboratories, #C06SB) and 0.2% TritonX-100 for at least 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... on Criterion 4–12% precast gels (Bio-Rad). Wells were loaded with the total eluent volume from the IP samples ...
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bioRxiv - Synthetic Biology 2022Quote: ... SDS-PAGE gels (4–15% acrylamide, Bio-Rad) and Phos-tag™ gels were transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... 66.7 µl 4× Laemmli sample buffer (Bio-Rad), supplemented with 0.2 M dithiothreitol ...
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bioRxiv - Physiology 2024Quote: ... separated on 4-20% gradient gels (Bio-Rad) by SDS page and subsequently transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... Anti-Ly6B (7/4) was purchased from BioRad. DAPI was included for samples being analysed by Amnis FlowSight ...
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bioRxiv - Developmental Biology 2024Quote: ... 4-20% mini-PROTEAN® TGX (Biorad, #4561094) were loaded with 10uL of sample and western blot analysis were performed according to standard protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... and denatured in 4× Laemmli buffer (Biorad 1610747). Whole cell extracts were resolved by 8–16% Tris-glycine polyacrylamide gel (Invitrogen XP08165BOX) ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg/ml Coomassie Brilliant Blue R250 (Biorad), 5 μg/ml Crystal Violet (MilliporeSigma)) ...
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bioRxiv - Physiology 2024Quote: ... separated on 4-20% gradient gels (Bio-Rad) by SDS page and subsequently transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... TGx Mini-PROTEAN 4-15% precast gel (BioRad), or Bolt Bis-Tris Plus mini-Protein Gel 4-12% (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... separated on 4%-12% SDS-PAGE gels (BioRad), and transferred to PVDF membranes (Trans-blot turbo transfer system ...
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bioRxiv - Neuroscience 2024Quote: ... Precast 4-15% TGX protein gels (Bio-Rad) were loaded with 10-20 μg of total protein lysate and run for 1h at 135V ...
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bioRxiv - Genomics 2024Quote: ... 4× Laemmli Sample Buffer (Bio-Rad, no. 1610747) supplemented with 10% β-mercaptoethanol (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... and mouse hypoxanthine-guanine phosphoribosyltransferase gene (Hprt) (forward, 5’-GGACTTGAATCAAGTTTGTG-3’; reverse, 5’-CAGATGTTTCCAAACTCAAC-3’) in a CFX96 Real-Time PCR Detection System (Bio-Rad). The qRT-PCR results were analyzed using the ΔCt method (relative expression ...
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bioRxiv - Microbiology 2023Quote: ... US28 (sense, 5’-CCAGAATCGTTGCGGTGTCTCAGT-3’; antisense, 5’-CGTGTCCACAAACAGCGTCAGGT-3’) and GAPDH were detected with CFX Connect Real Time System (Bio-Rad) using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... and primer set previously described [25] CtPl+ 5’-TAGTAACTGCCACTTCATCA-3’ and CtP2 5’-TTCCCCTTGTAATTCGTTGC-3’) and using a CFX96TM real-time thermocycler (Bio-Rad)) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... Samples were run on a precasted gradient gel (4 – 15% Criterion TGX, 12+2 wells, 45 μl, BioRad, CA, USA) for 45 min at 60 mA ...
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bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Cell Biology 2024Quote: ... IP samples or cell pellets resuspended in SDS sample buffer containing 2-mercaptoethanol were subjected to SDS-PAGE using a 4%-15% pre-cast gradient gel (BioRad), and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... They were incubated at the room temperature for 5-10 min and they were placed inside a 4-mm cuvette (Bio-Rad). The cuvettes were pulsed using the BTX electroporator (300 V ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... excess antibody subsequently removed by a further 4 washes in TBSTw for 5 minutes before detection with ECL Western blotting reagent (Bio-Rad) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of neuronal lysate and corresponding mHTTex1 seed were boiled for 5 min with sample loading buffer and loaded into a 4-20 % polyacrylamide gel (SDS-PAGE, Criterion Bio-Rad) to detect monomeric endogenous HTT or by semi-denaturing detergent agarose (1.5% ...
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bioRxiv - Microbiology 2020Quote: ... and boiled for 5 min prior to loading on 4-20% SDS-PAGE gradient gels (Mini-PROTEAN TGX precast gels; Bio-Rad). Precision Plus Protein Unstained Protein Standards (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled for 5 min and the proteins were separated in 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-RAD) and electroblotted to nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... cell density was between 4-5 × 106 cells/mL with >95% viability as determined using a TC20 automated cell counter (Bio-Rad). Cells were diluted with warm Expi293 Expression Media to 2 × 106 cells/mL in 42.5 mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
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bioRxiv - Developmental Biology 2022Quote: ... The suspended eggs and siRNA (5 μg/μl stock solution, in MilliQ) were transferred to a 4 mm cuvette (Bio-Rad) with a final volume of 200 μl and were gently mixed ...
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... The second-dimension gel electrophoresis was performed in 1× TBE buffer containing 0.3 μg/ml of ethidium bromide at 6.0 V/cm for 5 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was transferred to Hybond-XL (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...