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Citations for Bio-Rad :
2901 - 2950 of 4293 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 Integrase 2E3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... for 1 minute at RT and imaged on the ChemiDoc system (Bio-Rad, #17001401). Protein expression was normalized to housekeeping genes and quantified using ImageJ ...
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bioRxiv - Molecular Biology 2024Quote: ... This solution was rapidly combined with 40 % acrylamide (37.5:1 mono:bis) solution (Bio-Rad) (sufficient for a final concentration of 2 %) ...
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bioRxiv - Immunology 2024Quote: ... transferred to 1 ml columns with pre-wetted bottom frit (Bio-Rad, cat. 7326008) and further washed three times with 1 ml WB by gravity flow ...
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bioRxiv - Microbiology 2024Quote: ... 80 µL was mixed with 320 µL 1% agarose (Bio-Rad Certified Megabase Agarose) at 45 C ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 µL of a 1:3000 dilution of HRP- conjugated protein G (Bio-Rad) and 100 µL of HRP substrate buffer (20 ml NaCitrate pH 4.5 + 1 ml ABTS + 0.4 ml H2O2 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times for 10min with 1xTBS-T and then incubated with HRP-coupled anti-rabbit secondary antibodies (170-6515, Biorad) for 1h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Tween 20 (TBS-T) in 5% (w/v) milk (pH 7.4) followed by incubation with an anti 6xhistidine antibody (Bio-Rad) overnight ...
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bioRxiv - Immunology 2019Quote: ... After quantifying the optimal virus-to-erythrocyte concentration (4HA units), serial two-fold dilutions of Fc, control IVIG (GammaGard, Baxter Healthcare) and polyclonal goat anti-influenza B (Biorad) were prepared ...
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bioRxiv - Cell Biology 2022Quote: ... and affinity purified anti-HSP-90 antibodies by use of an MBP-HSP-90 antigen coupled to Affigel matrix (BioRad), using a procedure previously published (Benian et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... transferred to nitrocellulose membranes and blotted with either polyclonal rabbit anti-GFP (EU2; Eusera) and Tidyblot Western Blot Detection Reagent:HRP (STAR209P; Bio-Rad) or monoclonal mouse anti-HA (MMS-101R ...
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bioRxiv - Microbiology 2019Quote: ... followed by incubation with EndoS34 or SpeB68 antiserum The membranes were washed followed by incubation with a secondary antibody (goat anti-rabbit HRP-conjugated antibody, BioRad). The membranes were developed using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... After incubation with the secondary antibody (goat anti-rabbit IgG HRP conjugate, JD111036047) 5mC signal was detected using the ChemiDocTM XRS+ Imaging System (Biorad) and analyzed using Image LabTM Software (Biorad) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Bovine Interleukin-8 PBP039 and Goat anti Bovine Interleukin-8 Ab conjugated to biotin AHP2817B (all from Bio-Rad, protocol according to the manufacturer’s intructions).
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bioRxiv - Cell Biology 2022Quote: ... and probed with designated primary antibodies followed by a goat anti-rabbit IgG HRP-conjugated secondary antibody (BioRad, Hercules, CA). Bands were visualized using SuperSignal reagent (Thermo-Fisher ...
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bioRxiv - Immunology 2022Quote: ... Transfectants were stained for viability using a Live dead blue dye followed by anti-HLA DR DP DQ antibodies (BioRad clone Bu26 ...
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bioRxiv - Immunology 2023Quote: ... IgA and IgG antibody isotypes were detected by horse reddish peroxidase (HRP)-conjugated goat anti-pig antibodies specific for porcine IgA (clone AAI40P, BioRad) diluted 1:5 000 ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was visualized by incubating the membrane with an HRP-labelled anti-rabbit antibody followed by detection using ECL (Biorad) at the Chemidoc (Biorad).
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with an Alkaline Phosphatase-conjugated Anti-Digoxigenin antibody and the chemiluminescent signal was revealed using a ChemiDoc MP imaging system (Biorad).
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... CldU or IdU were detected by immunofluorescence with either anti-BrdU antibody that recognizes CldU (MCA2060, Bio-Rad Laboratories, Hercules, CA) or anti-BrdU antibody that recognizes IdU (347583 ...
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bioRxiv - Microbiology 2020Quote: ... anti-GFP antibody was used and bound primary antibody was detected using anti-rabbit IgG-HRP secondary antibody following visualization with the Clarity™ Western ECL Substrate (BioRad) and subsequent detection in a ECL Chemocam Imager (Intas Pharmaceuticals Limited) ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were incubated with HRP-conjugated secondary antibodies for both FLAG and GFP stainings (Rabbit anti-goat-HRP conjugate, #1721034, Bio-Rad) diluted at 1:5000 ...
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bioRxiv - Bioengineering 2019Quote: ... The anti-SSTR2 mAb was purified using our two-step antibody purification protocol by the NGC system (Bio-Rad, Hercules, CA) equipped with Protein A column and ion exchange column.(34 ...
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bioRxiv - Microbiology 2022Quote: ... Bound IgG1 and IgG2 antibodies were detected by adding 100μl/well sheep anti-bovine IgG1 or IgG2 HRP (Bio-Rad, USA) at 1:5000 and 1:2500 respectively.
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bioRxiv - Biophysics 2022Quote: ... Aqueous solutions in BRB80 pH 6.9 were introduced in the chamber in the following sequence: 20 μL of 0.05 mg/mL anti-tubulin antibody (Bio-Rad Laboratories) for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were washed three times with T-TBS for 15 min and incubated with goat anti-rabbit HRP-conjugated secondary antibodies (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: 100 µL of well mixed anti-FLAG M2 Affinity Gel aliquots were loaded on columns (Micro Bio-Spin Columns, Bio-Rad) and washed two times with 1 mL of cell lysis buffer by gravity flow ...
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bioRxiv - Molecular Biology 2019Quote: ... 80 μL of the supernatant was removed (termed ‘whole cell extract [WCE] sample’) and 5 μg of antibody (anti-PK (Bio-Rad) or anti-HA (Roche) ...
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bioRxiv - Pathology 2020Quote: ... proteins were subjected to primary antibody recognition using an anti-P1 antibody raised in rabbit (Siré et al, 2008) and purified by Affi-Gel 15 immnunoaffinity chromatography (BIO-RAD) using the recombinant P1 protein as the ligand (used at a 1:1000 dilution) ...
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bioRxiv - Neuroscience 2019Quote: ... and lysed then immunoprecipitated as described above using homemade rabbit anti-PGRN antibodies bound to Affi-Gel 15 (Bio-Rad Laboratories). Samples were then mixed and boiled 5 minutes with 10mM DTT followed by alkylation by treating samples with a final concentration of 28 mM iodoacetamide ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates of WT OSC were incubated with 1μg of Rabbit anti-Piwi polyclonal antibody (18) and 30 μl Surebeads Protein A Magnetic beads (Bio-Rad, 1614013). Immunoprecipitation was performed at 4°C overnight ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... The membrane was incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies in 5% milk and bands were developed with Chemi-Doc XRS imaging (Bio-Rad). The primary antibodies used are listed in Supplementary Table 3.
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and then incubated with the secondary antibody (Goat Anti-Rabbit IgG – HRP conjugate, Bio-Rad cat# 1706515) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and followed by one-hour incubation with diluted secondary antibody (1706515 Goat Anti-Rabbit IgG-HRP Conjugate, Bio-Rad, Hercules, CA). Protein lysates from each cell line were analysed on multiple gels (four per cell line ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were washed and shaken in PBST thrice and then re-probed with a secondary goat anti-rabbit IgG-HRP-conjugated antibody (172-1019, Bio-Rad) at 1:10000 ...
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bioRxiv - Microbiology 2024Quote: ... Blots were incubated with rabbit antisera raised against purified ShfP or σA and detected using anti-rabbit IgG StarBright (Bio-Rad) with a ChemiDoc MP imager (BioRad).
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bioRxiv - Neuroscience 2023Quote: ... and the supernatants were incubated with either 2 µg of anti-PAR antibody (clone #19, custom designed at AbD Serotec, Bio-Rad) or Human IgG F(ab)2 (Rockland ...
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bioRxiv - Immunology 2023Quote: ... The role of Mincle was investigated by incubating the cells with custom-made HuCAL anti-bovine Mincle CRD antibodies (AbD31621 and AbD31662) and HuCAL Fab-dHLX-FH Negative control (Bio-Rad) at 20 µg mL-1 for 15 min prior to addition of GFP-BCG.
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bioRxiv - Microbiology 2023Quote: Membrane was then washed three times with TBS-T and incubated with secondary antibody (Goat Anti-Rabbit IgG StarBright Blue 700 by Bio-Rad) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Immunology 2023Quote: ... the eluted samples were dot-blotted and UV cross-linked to a nitrocellulose membrane that was immunoblotted with anti-BrdU (BioRad #AHP2405), anti-8OHdG (Thermo Fisher #BS-1278R ...
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bioRxiv - Cell Biology 2023Quote: ... The analyses were performed in duplicate with 100nM of both sense and anti-sense primers in a final volume of 20 µL using iQTM SYBR Green Supermix (Bio-Rad). Specific primers were designed using Primer3 (Supplementary Table 1-2) ...
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bioRxiv - Microbiology 2023Quote: ... Signal detection was conducted with a goat anti-rabbit-HRP secondary antibody followed by development with Clarity ECL Substrate (Bio-Rad). Blots were imaged on Chemidoc XRS Imaging System (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Cell Biology 2024Quote: ... Signal intensities of anti-HA-immunoreactive bands representing tGnd1-HA and stGnd1-HA were normalized to the Stain-free signal (BioRad Laboratories) of the total proteins originating from the whole lane ...
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bioRxiv - Genetics 2020Quote: ... quantitated and 1 μg of RNA reverse transcribed into cDNA using iScript kit (Bio-Rad). Three overlapping MTM1 cDNAs were amplified using three pairs of cDNA primers from Tosch et al(Tosch et al ...
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bioRxiv - Cell Biology 2020Quote: ... 0.01% (w/v) bromophenol blue) and loaded on a 5% poly-acrylamide (29:1, BioRad) 0.5 x TBE gel ...
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bioRxiv - Developmental Biology 2020Quote: ... secondary antibody diluted in TBST containing 0.5% BSA with streptavidin-HRP (Bio-Rad, 1:10,000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 1 µL cDNA was used to perform qPCR using SsoAdvanced SYBR Green supermix kit (BioRad) on a CFX96 machine (BioRad) ...