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Citations for Bio-Rad :
2851 - 2900 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated using 1 μg of RNA and the iScript cDNA Synthesis Kit (Bio-Rad, #1708891). qPCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891). Intron-spanning primers and probes for cep290 and ef1a1l1 were designed with Primer3Plus (http://primer3plus.com) ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was generated from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad). Assays were performed with PerfeCTa SYBR Green SuperMix ...
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bioRxiv - Plant Biology 2021Quote: ... 1-2μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis kit (BioRad) or StarScript II First-strand cDNA Synthesis Kit (GeneStar) ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 1 μg RNA using the iScript Advanced cDNA Synthesis Kit (Bio-Rad, #1725037). For the 3-12 mo trial ...
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bioRxiv - Molecular Biology 2021Quote: 1 μg Turbo DNase-treated RNA was reverse transcribed with the iScript cDNA synthesis kit (BioRad, 1708890), treated with 0.5 μL RNase cocktail (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... followed by streptavidin-alkaline phosphatase (1 µg/mL) and development with AP conjugate substrate kit (Bio-Rad). For analysis of intracellular cytokine production ...
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bioRxiv - Developmental Biology 2022Quote: 1 µg of RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Cat# 1708891, Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 ug of RNA using the iScript cDNA Synthesis Kit (BioRad #170-8891). Reverse Transcriptase Quantitative PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg total RNA was subjected to reverse transcription with the iScript cDNA synthesis kit (Bio-Rad). In case of RNA isolated with TRIzol ...
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bioRxiv - Neuroscience 2023Quote: 1 to 0.5 μg of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). A volume corresponding to 5 ng of initial RNA was employed for each real-time PCR reaction using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... RNA (0.5 to 1 μg) was reverse transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad), and real-time PCR reactions were performed as described previously10 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 µg RNA was used to synthesize cDNA using the iSCRIPT cDNA kit (Biorad, Hercules, USA) according the manufacturers description.
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bioRxiv - Molecular Biology 2023Quote: 1 μg Turbo DNase-treated RNA was reverse transcribed with the iScript cDNA synthesis kit (BioRad, 1708890), treated with 0.5 μL RNase cocktail (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 1 µg RNA using iScript gDNA Clear cDNA Synthesis Kit (BIORAD, Hercules, CA), according to manufacturer’s instructions and the cDNA was diluted to 5 ng/µl.
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized from 1 μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using the LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... RNA (1 μg) was then converted to cDNA using an iScript cDNA Synthesis Kit (Bio-Rad: #1708891BUN), which was then analyzed on a BioRad CFX96 Real Time Thermocycler using 2X SYBR green qPCR master mix (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... and cDNA was prepared from 1 μg of RNA using the iScript cDNA synthesis kit (Bio-Rad), both according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2023Quote: ... 1 μg of RNA was used for cDNA synthesis with iScript cDNA Synthesis Kits (BioRad, Hercules, CA) in a 20μl reaction volume ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 1 µg RNA samples using the iScript cDNA Synthesis kit (Bio-Rad, USA). qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of RNA was used for reverse transcription using an iScript cDNA synthesis kit (Bio-Rad). cDNA was diluted 5 times in Milli-Q water and used as a template for PCR amplification using GoTaq G2 Green Master Mix (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of total RNA was converted into cDNA with iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and 50 ng of cDNA was used in each reaction ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from ∼ 1 μg of extracted RNA using an iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed for IFNB and IL-1β on the Applied Biosystems PRISM 7500 qPCR machine ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... Concentrations of the purified proteins were determined by Bradford Assay using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad 5000006) and calculating the ratio of the samples’ absorbance at 595 nm and 472 nm ...
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bioRxiv - Immunology 2021Quote: ... Molecular weight changes of the antigen were evaluated by reducing SDS-PAGE and protein bands were visualized with high sensitivity Flamingo Fluorescent Protein Gel Stain (1610491, Bio-Rad Laboratories). Intact antigen fractions were determined by digital imaging of gels followed by ImageJ analysis ...
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bioRxiv - Immunology 2021Quote: ... These tubes were then centrifuged at 300G for 5min at 4C and the supernatants were used for protein quantification using the DC protein quantification assay protocol (Bio-rad 5000112). After incubation at −80 °C for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured protein extracts were resolved using 4-9% SDS-PAGE (4–15% Mini-PROTEAN® TGX™ Precast Protein Gelscat# 4561083, Biorad) and then transferred to a nitrocellulose membrane.
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were centrifuged in v-bottom 96-well plates rather than microcentrifuge tubes and total protein concentration was determined using Protein Assay Dye (BioRad #500-0006) following standard protocols ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration of the supernatants was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-rad Laboratories, Inc., CA, USA); the absorbance was measured at 595 nm with a Thermo Scientific Multiscan® GO (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
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bioRxiv - Plant Biology 2021Quote: ... The total protein concentration of each sample was calculated by a Bradford assay following the Bio-Rad Protein Assay protocol (Bio-Rad, USA), and protein concentrations adjusted ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were analyzed on 10% Tris-Tricine SDS-PAGE system (38) with Precision plus protein standard (Bio-Rad 1610363) as a molecular weight marker ...
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bioRxiv - Biochemistry 2022Quote: ... The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Biorad). Fractions containing high molecular weight species were pooled together and concentrated to <500 µL using a 100-kDa cutoff concentrator ...
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bioRxiv - Biochemistry 2021Quote: ... Harvested cells were lysed in TNI lysis buffer and 10 μg of protein (as measured using Bio-Rad protein assay dye reagent) was aliquoted and precipitated via methanol/chloroform precipitation as described below ...
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bioRxiv - Physiology 2022Quote: ... supernatants were transferred to new Eppendorf tubes and protein concentrations were then determined using a DC protein assay (Bio-Rad, Hercules, CA). 750 µg of protein was diluted in 4X Laemmli sample buffer (LSB ...
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bioRxiv - Molecular Biology 2019Quote: ... The IgG fraction was purified from hybridoma culture medium by Protein A chromatography on a Profinia™ protein purification system (Bio-Rad) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration was determined in triplicate using a Bradford assay (Bio-Rad protein assay dye reagent concentrate, Bio-Rad Laboratories, Hercules, CA), against bovine serum albumin standards (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... followed a method adapted from our previous research.38 Protein concentrations were determined using the DC Protein Assay (Bio-Rad, Hercules, CA).
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bioRxiv - Plant Biology 2021Quote: ... Plant leaf protein samples (30-100 µg) were run on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Physiology 2019Quote: ... 25 µg of protein extract per sample was separated on 12% acrylamide gels (#5678044, Criterion™ TGX Stain-Free™ Protein Gel, Biorad) and blotted on PVDF membranes (Immobilon-FL IPFL00010 ...
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bioRxiv - Genomics 2021Quote: ... Both total and mitochondrial protein concentrations were determined using the Bradford method (Bio-Rad protein assay, Bio-Rad Laboratories GmbH, München, Germany). Protein extract volumes equivalent to 50–75 μg of total protein were concentrated into smaller volumes by lyophilizing the samples with a Savant Speed Vac Plus SC210A centrifugal evaporator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was transferred to a new tube and total protein concentration was determined by Bradford protein assay (BioRad, C500-0205). For each condition ...
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bioRxiv - Cancer Biology 2021Quote: Fifteen µg of protein per sample was separated on a BioRad Mini-protein 4-15% precast 12 well 20 µl gels (4561085, Bio-Rad, CA) and electrophoretically transferred to Odyssey Nitrocellulose membrane (P/N 926-31092 ...
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bioRxiv - Microbiology 2021Quote: ... protein pellets were resuspended in 100 µL of 6M urea in 50mM ammonium bicarbonate for DC® protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Immunology 2022Quote: ... The protein was concentrated using a 0.5 Centrifugal Filter Unit (Amicon) and then run on a Precast Protein Gel (Bio-Rad, 456-1103) (98 ...