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Citations for Bio-Rad :
2801 - 2850 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 10-50 μg of protein was loaded onto 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and transferred to polyvinylidene difluoride (PVDF ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Molecular Biology 2021Quote: ... equal amounts of protein for each sample was loaded on 4-15% Criterion TGX pre-cast midi protein gel (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Bioengineering 2021Quote: Bradford assays were performed to quantify the concentration of total soluble protein (TSP) by using a protein assay dye reagent (Bio-Rad). For each BSA standard ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was estimated colorimetrically using a Bio-Rad protein assay and 25 μg of protein with equal volume of 2X Laemmli sample buffer (Bio-Rad) was denatured at 70°C for 10 min ...
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bioRxiv - Immunology 2021Quote: Lysates were centrifuged for 15 minutes at 4°C to recover supernatant and quantified for protein concentration using Protein Assay Dye Reagent Concentrate (Bio-Rad). 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Purified products were analyzed by 10% TBE PAGE and total protein quantified with Bio-Rad protein quantification reagent (Bio-Rad, 5000006). LPS contamination after conjugation was below 0.5 EU/mg as mentioned below in the vaccinations section.
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bioRxiv - Microbiology 2022Quote: ... Equivalent protein amounts (EVs and proteins) were separated on 4-15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... the protein concentration in the supernatant was assayed by Bradford’s method and 60ug of protein/sample was electrophoresed (Bio-Rad, USA) on a 5%/10% (loading/separating ...
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bioRxiv - Biochemistry 2022Quote: ... The protein concentration was measured on Tecan (Schoeller) and approximately 20 μg of protein lysate was loaded on the 10-well protein gels (Mini-PROTEAN TGX, Bio-Rad) and run at 120 V ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on 10% or 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Pierce™ Protease& Phosphatase Inhibitor Mini Tablets) and final protein concentration was determined with BCA protein Assay (Biorad Laboratories, Feldkirchen, Germany). Heart tissue samples of the infarct areas were excised (2 weeks or 8 weeks post-surgery ...
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bioRxiv - Biophysics 2024Quote: ... total proteins and biotinylated proteins were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Microbiology 2024Quote: ... The same amounts of proteins were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat:4561096) or homemade 6-15% SDS-PAGE gels and transferred to a nitrocellulose membrane (Biorad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein purity was analyzed by SDS-PAGE and the protein concentration was determined using the Bradford assay (QuickStartTM, Bio-Rad, Germany) with BSA as the protein standard (Zor & Selinger ...
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bioRxiv - Microbiology 2024Quote: ... These concentrations were used to load equal sample of proteins into Any-kDTM Mini-PROTEAN® TGXTM Precast Protein Gels (BioRad) for SDS-PAGE ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein Gels (#4561094, Bio-Rad) and transferred to 0.2 μM PVDF membranes by a Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was used to quantify the total protein concentration in the sample by using the DC protein assay (Bio-Rad) and a BSA standard curve [54] ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein amount in each lysate sample was measured using a DC™ protein assay (Bio-Rad, Cat#5000113, #5000114, #5000115). Caspase-3 activity in each sample was calculated as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Totals of 10-30 µg of protein lysate and 10 µL of dual color protein standard ladder (Bio-Rad #161-0374) were loaded onto 4-20% Mini-PROTEAN gels (Bio-Rad #4568094 ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the DSG-crosslinked SATB2 interaction protein complexes were cleaved from Protein A/G beads by boiling in Laemmli Sample Buffer from Bio-Rad without adding reducing reagents (DTT or 2-Mercaptoethanol ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 μg of protein extracts were run on a 4%–15% polyacrylamide gels (Mini Protein TGX Stain-Free gels, Bio-Rad) and transferred to PVDF (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The post-nuclear fraction was collected and protein concentration was determined by the Bradford protein assay (Bio-Rad Laboratories, Hercules, USA) using bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2023Quote: The flash-frozen purified proteins were thawed on ice and the protein concentration was estimated using Bradford reagent (Bio-Rad, USA). Ten µg of the purified protein was boiled in 1X sample buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins ≤ 250 kDa were separated by SDS-PAGE electrophoresis using 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and transferred to Immobilon-P PVDF Membrane (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was quantified by Qubit and 15ug protein was run on a 4-20% Mini-PROTEAN(R) TGX Stain-Free Protein gel (Bio-Rad). Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was loaded for each sample (20 to 40 μg of protein lysates) and diluted with Laemmli Sample Buffer (Biorad, 1610747) and RIPA buffer in a final volume of 30 μL and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 10 µg (for FLN-DD experiment) of protein lysates for each treatment condition was fractionated in 4-15% Mini-Protean TGX protein gels (Bio-Rad) and transferred to nitrocellulose membrane using Bio-Rad Trans-Blot Turbo Transfer System with “Turbo Mixed MW” settings (constant 2.5A for 7 minutes) ...
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bioRxiv - Plant Biology 2023Quote: Protein samples of whole cell extracts (0.5 µg chlorophyll or 10 µg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: The protein of the lysates was then adjusted to 20 mg total protein using 4x Laemmli Sample Buffer (#1610747, BIO-RAD). The protein sample was denatured and run on 4-15% Mini-PROTEAN TGX Precast Protein Gels as per the manufacturer’s instructions (BIO-RAD) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the concentration of the protein was determined either by SDS-PAGE or by Bradford assay using protein assay dye reagent (Bio-Rad) and bovine serum albumin (Carl Roth ...
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bioRxiv - Physiology 2023Quote: ... The resulting lysates were used for quantification of the total protein content with the DC protein assay (Bio-Rad, Hercules, USA) according to Lowry et al ...
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bioRxiv - Microbiology 2023Quote: ... 15 µg of membrane and soluble fraction were loaded after normalization by total protein quantification using the Bio-Rad Protein Assay (Bio-Rad) onto a 12% acrylamide gel ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were visualized via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Precision Plus Protein Dual Color Standards (Bio-Rad) at volumes normalized to the initial hair mass ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and purity of extracted protein was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006) following manufacturer’ s protocol.
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bioRxiv - Cell Biology 2024Quote: The protein extracts were separated by 10% SDS-PAGE and the proteins were transferred on the low fluorescence PVDF membrane (Bio-rad). The molecular weight of the proteins was assigned by Precision Plus Protein Dual Color Standards (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 10 µL of each protein sample was then loaded and run on precast protein gels (4-20% Mini-PROTEAN® TGXTX, BioRad) before staining with Coomassie blue dye ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories, Inc., CA, USA). ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized from 1 μl/RNA sample using an iScript kit (Bio-Rad, catalog #1708891), and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription using the iScript cDNA synthesis kit (Bio-rad). The gene expression analysis was carried out using the SsoAdvancedTM Universal SYBR Green Supermix (Bio-rad ...
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bioRxiv - Bioengineering 2019Quote: ... and 1 μg of RNA was transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). Primer pairs MFS17/MFS18 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of RNA was reverse transcribed into cDNA using the Reverse Transcription Kit (Bio-Rad, USA). qRT-PCR was carried out using SYBR Green (Roche ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was retro-transcribed using the iScript cDNA Synthesis Kit and polydT primers (Biorad). The cDNA was amplified using the primers listed in S2 Table and the SsoAdvanced universal SYBR Green supermix (Biorad) ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 µg of total RNA was converted to cDNA using iScript Select cDNA Synthesis kit (Bio-Rad). Quantitative RT-PCR was performed in triplicate using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was prepared from 1 ug of purified RNA using the iScript cDNA Synthesis Kit (Bio-Rad) as stated in the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA synthesis was performed with 1 μg of total RNA using iScript cDNA Synthesis Kit (Bio-Rad) and diluted 10-fold ...