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Citations for Bio-Rad :
2851 - 2900 of 4034 citations for 7 3 Chloropropoxy 4 chloroquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
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bioRxiv - Developmental Biology 2019Quote: Oocytes were collected in 0.1% PVP in DPBS and boiled for 3 mins at 95°C in 1x Laemmli Sample Buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... (see Supplementary Table 3 for a full list of oligos used in the present study) into 384-well hard-shell PCR plates (Bio-Rad HSP3901) using Mantis liquid handler (Formulatrix) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated to 50°C for 2 to 3 min before the addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexed vigorously for 5 sec before pipetting in plug mould (Bio-Rad 1703713) ...
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bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2020Quote: ... Two microgram of concentrated viruses were resolved on 4-20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (Biorad) using the Novex™ Sharp Pre-stained Protein Standard (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were cooled briefly and loaded onto 4-20% pre-cast gels (Bio-Rad Mini-PROTEAN TGX Gels Cat# 4561095) and run at 100-120V using the Bio-Rad Mini-PROTEAN Tetra System ...
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bioRxiv - Systems Biology 2020Quote: ... Immunoblotting was performed using SDS-PAGE with 12.5% acrylamide or 4-15% gradient gels (Bio-Rad, Cat #4561046, Cat #4561086).
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: Samples were resolved on Mini-Protean® TGX 4–20% gels or TGX 7.5% gels (BioRad Laboratories, 4568095 and 4568025). Separated proteins were transferred to Trans-Blot Turbo 0.2-μm PVDF membranes (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... In short: OCT is removed with PBS and then tissue is placed in a cold 4% polyacrylamide (1610140 Bio-Rad) hydrogel with 0.25% photoinitiator (VA-044 ...
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bioRxiv - Cell Biology 2020Quote: ... Lyophilized proteins were dissolved in SDS-PAGE sample buffer and resolved by SDS-PAGE (BioRad precast 4-15 % gradient gel). The gel was stained with Simple Blue Safe Stain (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein extracts were resolved on 4-15% Criterion™ Tris-HCl precast gels (#3450029, Bio-Rad Laboratories, CA, USA) and blotted onto PVDF membranes (#1704157 ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Microbiology 2019Quote: ... Fractions of 1 ml were recovered from the top to the bottom of each tubes and loaded on a gradient 4-12 % SDS-PAGE Bis-Tris gel (BioRad). For Western Blot analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were prepared for mass spectrometry by disrupting total or immunopurified ribosomes in 1X Laemmli buffer and running on a 4-20% Mini-PROTEAN TGX Precast Protein Gel in 1X Tris/Glycine/SDS buffer (BioRad) for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 OD equivalents of the resulting sample was subjected to SDS-PAGE and the proteins were separated on 4-15% Mini-PROTEAN-TGX strain-free gels (BioRad). For subsequent immuno-blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... Octamers were visualized using 18% separating (4% stacking) discontinuous Laemmli SDS-PAGE in Mini-Protean gel running system (Bio-Rad) run for 70 minutes at 22mA ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected for BCA assay and analyzed by SDS-PAGE using Mini-Protean® TGXTM 4– 15% (BioRad) and transferred to nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... separated by SDS-PGE on a 4-15% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-Rad #456-8084), and transferred with the Trans-Blot® Turbo™ RTA Transfer Kit (Bio-Rad #170-4275 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated on 4-20% SDS electrophoresis gel and transferred onto PVDF membranes using Trans-Blot Turbo Transfer System (#1704156, Biorad). Images were acquired using Chemidoc Imaging system (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... and equal amount of proteins were separated by SDS/PAGE on pre-cast 4-20% polyacrylamide gradient gels (Cat# 5671094, Biorad) and transferred to immune-blot PVDF membrane (Merck cat# IPVH00010) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by gel electrophoresis on a 4 to 20% polyacrylamide gradient Mini-Protean TGX precast gel (Bio-Rad) and transferred to an Immobilon-P membrane (Millipore) ...
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bioRxiv - Genetics 2022Quote: ... from individuals with DMBT1 size isoform I-IV phenotypes were separated by electrophoresis on 4-15% gradient SDS-PAGE gels (BioRad) and subjected to Western blot-like binding of biotinylated bacteria ...
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bioRxiv - Molecular Biology 2020Quote: ... About 80 µg of denatured proteins were loaded for each condition and separated on gradient 4–12% polyacrylamide classic or TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Neuroscience 2021Quote: ... A 1:5 dilution from serum samples was combined with 4 °C Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: Whole flies or human primary cell pellet was homogenised in 2x Laemmli loading sample buffer (100 mM Tris pH 6.8, 20% glycerol, 4% SDS; Bio-Rad) containing 50 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were heated at 60°C and loaded on Mini-Protean TGX precast gels (456–1084, 4-15 %, Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 14,000×g for 30 min at 4°C and equalized using quick start Bradford 1x dye reagent (BioRad, 5000205). Ni-NTA resin was washed three times with lysis buffer containing 20mM imidazole pH 8.0 and 5mM β-mercaptoethanol while samples were prepared ...
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bioRxiv - Cell Biology 2022Quote: ... using the BioRad transfer system at 100 V for 60 min at 4°C or with Trans-Blot Turbo Mini PVDF Transfer Packs using Trans-Blot Turbo Transfer System (Biorad). The membrane was blocked for 30 min with the Odyssey Blocking Buffer (TBS ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants aspirated and loaded on to 4-12% NuPage Bis/Tris gels for separation before transfer on to a Nitrocellulose membrane (Biorad) at 4°C at a constant current of 350mA in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... were separated on a 4-20% SDS-PAGE gel and blotted onto a PVDF membrane using a Trans-Blot Turbo transfer system (BioRad). Membranes were blocked for 1h in 5% skim milk in TBST and then probed with an anti-S2 mouse monoclonal antibody (GeneTex ...
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bioRxiv - Cell Biology 2019Quote: ... Polyacrylamide gels used in this study were either prepared using 10% EZ-Run protein gel solution (Fisher) or precast gradient gels (4-20%, from Biorad). Any quantification performed on western blots was done using ImageJ software ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples (15 μL) were loaded onto pre-casted gradient (4-15%) SDS-polyacrylamide gels (Bio-Rad Laboratories; Hercules, CA, USA) and subjected to electrophoresis at 180 V for 60 minutes using pre-made 1x SDS-PAGE running buffer (Ameresco ...
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bioRxiv - Plant Biology 2019Quote: Proteins were separated on denaturing SDS-PAGE followed by an electrotransfer at 4 °C onto a PVDF membrane (0.2 µM, Bio-Rad). NRT2.1 was detected using three different anti-NRT2.1 antisera produced by Eurogentec against either the synthetic peptide TLEKAGEVAKDKFGK (anti-NRT2.1 19) ...