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Citations for Bio-Rad :
2801 - 2850 of 4034 citations for 7 3 Chloropropoxy 4 chloroquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg/sample was loaded onto 4-15% gradient polyacrylamide gels (Mini-PROTEAN TGX Gels, Bio-Rad, 4561083), transferred (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (30–50 μg) were loaded onto 4%–20% Mini-PROTEAN TGX Precast Protein Gels (BioRad). Proteins were separated by electrophoresis at 16 mA followed by transferring the proteins to Immobilon-P membranes (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (50 µg) were loaded into 4–20% PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat# 5678081). After overnight transfer to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL of the supernatant was loaded onto a 4-20% Mini-PROTEAN Tris-glycine gradient gel (Bio-Rad) and proteins separated by SDS-PAGE were transferred to a PVDF membrane ...
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bioRxiv - Biochemistry 2023Quote: Protein lysates were boiled in reducing sample buffer and resolved on 4-20% Criterion TGX polyacrylamide gels (Bio-Rad), then transferred to 0.2 µm nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µL samples were separated by 4-15% SDS-PAGE (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad). A mixture of recombinant proteins was used as a standard ...
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein were fractionated by SDS-PAGE (Mini-PROTEAN TGX Precast 4– 15% gradient gels, Bio-Rad), and eletro-transferred onto nitrocellulose membrane (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2024Quote: ... by boiling for 10 min at 98°C before loading onto a 4-20% gel (Bio-Rad, Hercules, CA), then transferred to PVDF 0.22 m membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of lysate was resolved via SDS-PAGE on a 4-20% gradient polyacrylamide gel (4561095; Bio-Rad) and transferred to a nitrocellulose membrane using the TransBlot Turbo mini-nitrocellulose kit (1704270 ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL of each sample was loaded onto a 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 180 V for 45 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled before being subjected to SDS-PAGE on Mini-PROTEAN TGX Precast Gel 4-15 % (Biorad, 4561086). Western blot using anti-SUMO antibody (non-commercial ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled before being subjected to SDS-PAGE on Mini-PROTEAN TGX Precast Gel 4-15 % (Biorad, 4561086). Western blot using anti-GFP (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... 20-30μg protein samples were loaded into 4-20% Mini-PROTEIN TGX Precast Protein Gels (Bio-Rad, Cat# 4561096), and then were transferred to 0.2µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Equal protein lysate concentrations (40μg per well) were loaded on a 4%-20% precast Mini-Protean gradient TGX gel (Biorad). Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were electrophoresed under reducing conditions on 4– 15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins (20 μg per sample) were separated on Mini-PROTEAN TGX 4–15% precast SDS-PAGE gels (Bio-Rad) for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... We loaded the lysates at equal protein amounts and concentrations on 4-15% precast TGX gels (Bio-Rad, USA) or 10% hand- poured SDS-PAGE gels ...
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bioRxiv - Immunology 2023Quote: ... and membrane GAS protein fractions were separated on SDS-PAGE (Criterion TGX Gels, 4%–20% precast gels, Bio-Rad) and proteins were transferred to PVDF membranes using the trans-blot turbo transfer system (BioRad ...
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bioRxiv - Biophysics 2023Quote: 10 µL of Dbp10 or Dbp10(R522V) pre-ribosomes at an OD260 absorbance of 5.0 were run on a 4– 20% denaturing Gel (BioRad) at 160 V for 7 min and stained with Coomassie Brilliant Blue R-250 (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 2.5% (v/v) beta-mercaptoethanol and ran on a 4-20% TGX gel (BioRad #5671094 and #5671095). Protein was transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2024Quote: ... The protein lysates were centrifuged and the supernatants were subjected to SDS-PAGE (gradient, 4 to 20%; Bio-Rad) and electroblotted onto a nitrocellulose membrane ...
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were separated by SDS–PAGE electrophoresis using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) followed by detection of the ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs) ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The same amount of protein was loaded in each lane of Mini-PROTEAN TGX gels (4–20%, Bio-Rad). The antibody signals were detected by chemiluminescence using ImmunoStar LD reagent (290-69904 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the samples were then boiled in the Laemmli buffer and loaded into 4%–20% gradient gel (Bio-Rad), transferred to PVDF membrane (Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were diluted to 4 M urea with water for Bradford Assay quantification (Bio-Rad Laboratories, Inc, Hercules, CA) and trypsin digestion ...
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bioRxiv - Biophysics 2024Quote: ... Equal volumes of protein samples were resolved on 4-12% gradient SDS-polyacrylamide gel and transferred onto 0.2μM nitrocellulose (BioRad #1620112) or PVDF (BioRad #1620177 ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on a 4 to 20% Trist-glycine stain free polyacrylamide 10-well gel (Bio-Rad) at 170 V for approximately 1 h in 1x Tris/glycine/SDS running buffer ...
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bioRxiv - Microbiology 2024Quote: Protein fractions were analyzed by standard denaturing SDS-PAGE electrophoresis using 4 to 20% gradient mini-gels (Bio-Rad), InstantBlue Coomassie protein stain ...
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bioRxiv - Neuroscience 2024Quote: ... Each sample (15 μg) was boiled for 5 min and applied on NuPAGE 4%– 12% Bis-Tris Gel (Biorad). The gel was transferred onto a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2024Quote: ... separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084), and the gel was stained using QC Colloidal Coomassie Stain (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... 40 µg of worm extract were loaded per well onto a precast 4-20%TGX gel (Bio-Rad, #4561094), and run in TGS buffer at 200 V for 45 min.
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bioRxiv - Neuroscience 2024Quote: ... Samples were loaded and run on 4-20% Criterion TGX Midi Protean gels (Bio-Rad #5671094, Hercules, CA, USA) and transferred to nitrocellulose 0.45 µm membrane (Bio-Rad #1620113 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated for 5 minutes (tissue ≤ 40μm) or 10 minutes (tissue ≥ 100μm) in polyacrylamide (PA) solution [4% acrylamide/bis acrylamide (BioRad., 1610154), 60mM Tris HCl pH 8 (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were analyzed with SDS–PAGE using 4-15% Mini-PROTEAN TGX Stain-Free Gels (4568083 Bio-Rad). Proteins were transferred to a nitrocellulose membrane (1704271 ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated by electrophoresis using 4-12% Bis-Tris mini protein gels and transferred to PVDF membranes (BioRad). Blots were incubated with primary antibodies at 4°C and then with HRP conjugated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate were separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BIO-RAD), transferred to polyvinylidene difluoride membranes (0.2 um ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1hr on ice with varying concentrations of fluorescently labeled hybridoma-derived or recombinant mAbs and then washed x3 in flow buffer (PBS + 3% fetal calf serum) prior to analysis on a ZE5 flow cytometer (BioRad, Hercules, CA). Equilibrium binding affinities were calculated via nonlinear regression (One site – total and non-specific binding ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml Tween20 add to 1000 ml with ddH2O) 3 times for 20 min and blocked with 10% dried milk-TBST solution or BlockAce-TBST solution (BlockAce, Bio-Rad, #170223) for 2 h ...