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Citations for Bio-Rad :
2801 - 2850 of 3233 citations for Recombinant Human Aldehyde Dehydrogenase 1 Family Member A3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1% SDS) and transferred onto nitrocellulose membranes (Protran) with a Trans-Blot SD semi-dry or wet transfer cell (Bio-Rad). Proteins of interest were probed using the appropriate primary and Horse Radish Peroxidase (HRP)-conjugated secondary antibodies (Jackson Immunoresearch) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with PBS-T for 5 min followed by 1 minute incubation with ECL detection reagent (Cytiva, RPN2106) and imaged with ChemiDoc MP Imaging System (Bio-Rad). Membranes were washed twice in TBS and stripped before reprobing according to stripping reagent manufacturer’s protocol (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... and incubated at 65°C for 3 mins before running 1% agarose-Etbr gel electrophoresis to detect the cleavage efficiency by ChemiDoc system (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Immunology 2022Quote: ... and primers to the editing locus producing amplicons of <500bp (1× assay, 900-nM primers and 250-nM probe) using ddPCR supermix for probes (no deoxyuridine triphosphate [dUTP]) (Bio-Rad). Reference reactions were simultaneously performed using a 5’ HEX labeled probe/ primer combination targeting the houskeeping gene B2M ...
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bioRxiv - Plant Biology 2022Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α (5’-CTG GAG GTT TTG AGG CTG GTA T-3’ and 5’-CCA AGG GTG AAA GCA AGA AGA-3’ ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse secondary antibodies (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Microbiology 2022Quote: ... and the RNA profile was measured by absorbance (254 nm) across the gradient in real time using an EM-1 Econo UV Monitor (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... and probed in triplicate by qPCR for the presence of the 1 kb capsule fragment using iTaq DNA polymerase (BIO-RAD) and primer pair DDL34-DDL35 ...
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bioRxiv - Microbiology 2022Quote: ... the generated cDNA was diluted (1:20) and amplified using stat1a- or stat1b-specific primers and 2x SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) in Hardshell® Low Profile Thin-wall 96-well skirted PCR plates (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL re-suspended Fraction B pellets were run 1 cm onto an Any kD Mini-Protean TGX gel (Bio-Rad) followed by Coomassie Blue staining ...
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bioRxiv - Neuroscience 2022Quote: ... First-strand cDNA synthesis was performed from 1 μg of RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad). Primers were designed using the NCBI Primer-BLAST tool and supplied by Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted 1:50 in water and subjected to RT-qPCR using the CFX384 Touch Real-Time PCR Detection System (BIO-RAD) and the ORA-qPCR Green ROX L 2X Mix (HighQu ...
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of diluted (1:8) cDNA were loaded for qPCR analysis along with the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725272) and 0.5 μM forward and reverse primers for target genes (listed below) ...
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bioRxiv - Zoology 2022Quote: ... RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad). Primers for five genes—Clock ...
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bioRxiv - Plant Biology 2022Quote: ... The dicing reactions were performed at 37 °C for 30 min and stopped by adding 1 volume TBE-Urea sample buffer (Bio-Rad), heating at 95 °C for 10 min and quickly chilling on ice for 2 min ...
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bioRxiv - Neuroscience 2022Quote: ... Single cell suspension was stored on ice in 1X EBSS+1%BSA while the live cell count/sample was determined using a TC20 Automated Cell Counter (Bio-Rad). For the extraction of metabolites 1x10^6 cells/sample were transferred into a 1.5ml Eppendorf tube ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were physically disrupted by adding 500 µL of 25:24:1 solution of phenol:chloroform:isoamyl alcohol and homogenizing in a bead beater (TeSeE Precess 48 Homogenizer, Bio-Rad, Hercules, CA) for 90 sec at 5300 rpm ...
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bioRxiv - Synthetic Biology 2024Quote: The samples (1 µL) were subjected to SDS-PAGE and transferred to a nitro cellulose membrane (Bio-Rad Laboratories, CA, USA). The membrane was blocked using 3% skim milk in PBST (137 mM NaCl ...
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bioRxiv - Plant Biology 2024Quote: pTRV1 and pTRV2-Del/Ros-1 harboring the SlATG7- or SlATG2-derived fragments were introduced into Agrobacterium strain GV3101 using electroporation (BIO-RAD). A 5 mL culture was grown overnight at 28°C in a medium containing kanamycin ...
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bioRxiv - Physiology 2024Quote: ... was used to wash the membranes before incubating with goat anti-rabbit horseradish peroxidase conjugated secondary antibody at 1:5000 dilution (1706515, Bio-Rad) for 90 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were mixed 1:6 with a loading dye and run on an agarose gel (1%) in Tris-acetate-EDTA buffer supplemented with 0.2 µG/mL of Ethidium Bromide (Biorad, Hercules, California). DNA was visualized under a short-wavelength UV light to confirm the lack of amplification for Heat-Killed Microbiome PMA-treated in comparison to the Heat-Killed Microbiome control (Supplementary Figure 2).
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bioRxiv - Pathology 2023Quote: ... Signal was visualized using a secondary horseradish-peroxidase-labeled goat anti-mouse antibody (goat anti-mouse IgG-HRP 1:5000, SantaCruzBiotechnology) and enhanced chemiluminescence (ECL Clarity Bio-Rad). The purity as well as equal loading (40γ ...
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bioRxiv - Microbiology 2024Quote: ... 1−10 ng of sample DNA was used for qPCR using a MiniOpticon qPCR detection system (Bio-Rad Laboratories, Hercules, USA). The iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of each total RNA preparation as template and the iScriptTM Reverse Transcription Supermix for qRT-PCR Kit (Bio-Rad). Primers listed in supplemental table S7 were employed for RT-PCR and qRT-PCR analysis of NtGEF12 transcript levels in comparison to reference gene expression ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodisc formation was initiated by adding washed Bio-Beads (200 mg dry weight per 1 mL of the protein mixture; Bio-Rad), and the mixture was rotated for two hours at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: Proteins from SDS-PAGE gels were transferred onto a PVDF (polyvinylidene difluoride) membrane (pre-activated in methanol for 1 min) using Trans-Blot Turbo transfer system (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... then incubated with ProSignal Pico Spray chemiluminescence substrate (Prometheus) for 1-3 minutes at room temperature and imaged using a ChemiDoc Imaging System (Bio-Rad). Contrast was occasionally adjusted to improve visualization of bands ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µL of each sucrose gradient fraction was incubated at 95 ºC with 1 % sodium dodecyl sulfate (SDS) and applied for SDS-PAGE with a 4-20 % gradient SDS-PAGE gel (Bio-rad). The proteins were transferred to a nitrocellulose membrane (Cytiva ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... All the reactants were run on 4.5% non-denaturing PAGE in 1× TBE and then Cy3 or Cy5 fluorescence signal was imaged by ChemiDoc MP imaging system (Bio-Rad). S8 Table presents details of the oligonucleotide sequence used for EMSA.
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... EtOH (p.a.)) and the Bio-Rad trans-blot® TurboTM Trans System (25 V, 0.5-1 A, 25 min; Bio-Rad, Germany). Afterward ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were then stained with Coomassie Brilliant Blue G (80 mg dissolved in 1 L water with 30 mM HCl) and destained with water before visualising using a ChemiDoc MP Imaging System (Bio-Rad). For mNeonTP-loaded encapsulins ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Microbiology 2024Quote: ... using a Superdex 30 Increase 10/300 GL (Cytiva) equilibrated with 50 mL water operated at 500 µL min−1 on an NGC medium-pressure chromatography system (Bio-Rad). Per chromatography ...
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bioRxiv - Cell Biology 2024Quote: ... and 1-1.5 µg RNA was used a template to make cDNA with the iScriptTM cDNA synthesis kit (Bio-rad #1708890), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed thrice and incubated with a secondary antibody (Goat anti-Mouse IgG-HRP, 1706516, Bio-Rad Laboratories, 1:10K), also diluted in 2.5% milk in TBST ...