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Citations for Bio-Rad :
2701 - 2750 of 3233 citations for Recombinant Human Aldehyde Dehydrogenase 1 Family Member A3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Then the samples were treated at 60oC for 1 h and then enzyme deactivated 95oC for 15 min in a thermocycler (Bio-rad). After the treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... qPCR analysis was performed using 1 μl diluted cDNA with biological (2-3) and technical replicates (2-3) using SsoAdvanced SYBR reagent (Bio-Rad) and Bio-Rad qPCR platform ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated in secondary antibody solutions (TBST with 5% Non-Fat Dry Skinned Milk and Horseradish Peroxidase conjugated secondary antibodies Goat Anti-Rabbit/Mouse, 1:10000, Bio-Rad) for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2020Quote: Organoids fixed in 4% w/v PFA and immunostained were embedded in 1% w/v low melting agarose (Bio-Rad Laboratories) solution and placed in an Eppendorf tube ...
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bioRxiv - Biophysics 2021Quote: ... The DDM/CHS detergent was then removed by overnight incubation with Bio-Beads SM-2 (15 mg per 1 ml protein/detergent/amphipols mixture, Bio-Rad) at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed according to the manufacturer’s instructions with minor modifications (SDS-PAGE run at 110 V, transfer onto PVDF membrane for 1 h at 110 V (BIO-RAD)) ...
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bioRxiv - Molecular Biology 2022Quote: ... at a 1:5 w/w ratio for 2 hours before addition of 100 mg mL-1 Bio-Beads SM-2 resin (Bio-Rad) and then incubated overnight at 4 °C with gentle agitation to remove detergent ...
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bioRxiv - Physiology 2022Quote: ... pH was brought back to neutral with 1 µL of TRIS buffer pH9 and samples were denatured in Laemmli buffer (Bio-Rad) at 95°C for 5 minutes and loaded for SDS-PAGE.
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bioRxiv - Genetics 2022Quote: Template cDNA pools were generated from 1 µg RNA using cells grown under standard glucose conditions (as above) by using iScript (Bio-Rad) RT with first-strand synthesis priming from a mixture of random hexamers and oligo-dT ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were separated on a pre-heated 45 cm sequencing gel (45 °C, 65 W, 1 h) and visualized using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... and the primers for murine IFNAR 1 and beta-Actin in a MyiQ™ Single-Color Real-Time PCR System (BioRad). Products of RT-PCR were separated by electrophoresis on a 2.5% agarose gel in 1x TBE buffer ...
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bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
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bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... All PCR reactions were run in triplicate using 1 μL of cDNA in a 25 μL final volume with iQ SYBR Green Supermix (Bio-Rad) and 200 nM each of forward and reverse primers (see Supplementary Table 5) ...
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bioRxiv - Bioengineering 2022Quote: ... All the eluted fractions were tested using a Bradford reagent assay (diluted 1:5 with ddH2O) (190 μL Bradford, 10 μL sample) (Bio-rad). Positive fractions were desalted using 100 kDa amicons (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary and HRP-secondary antibodies in TBST buffer with 5% milk (RT for 1 hour or overnight at 4 °C) and revealed with an ECL solution (BIO-Rad) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of SsoAdvanced Universal SYBR Green Supermix and 1 μl of the following DNA templates (Prime PCR Assay, Bio-Rad): mt-Co2 (mitochondrial ...
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bioRxiv - Plant Biology 2021Quote: ... The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad). Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration (Thermo ...
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bioRxiv - Immunology 2020Quote: Total RNA (up to 1 µg per reaction) from BMDM was reverse transcribed with iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative PCR was performed using 1 µl of cDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... and 25 µg of the targeting vector was transfected into 1 × 107 of Nmt1-heterozygous cells11 by electroporation (240 V, 500 µF) with Gene Pulser II (Bio-Rad). One week later ...
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bioRxiv - Biochemistry 2021Quote: ... and fractionated into 12 fractions (1ml each) using a Beckman fraction recovery system connected to an EM-1 UV monitor (Bio-Rad).
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bioRxiv - Plant Biology 2021Quote: ... and cDNA synthesized by reverse transcription of 1 µg of isolated total RNA using the iScriptTM Reverse Transcription Supermix (Bio-Rad) in a 20 µl reaction volume ...
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bioRxiv - Microbiology 2020Quote: ... membranes were subject to sequential incubation with anti-FLAG mouse antibody (1: 2000 dilution) and secondary anti-mouse IgG conjugated to HRP (Bio-Rad). After washing three times with TBST buffer ...
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bioRxiv - Microbiology 2020Quote: ... baumannii cells using a Bio-Rad GenePulser II (1.8 kV, 25 μF, and 200 Ω) in a 1 mm electrode gap cuvette (Bio-Rad). Cells were recovered by resuspension in 1 mL of SOC medium and incubated at 37°C with shaking (200 rpm ...
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bioRxiv - Pathology 2021Quote: ... The sections were then incubated in blocking buffer (10% goat serum; 1 hour) followed by incubation with either rat anti-F4/80 antibody (Bio-Rad) diluted 1:500 or rat anti-CD68 antibody (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Each cDNA sample was diluted 1:15 and used directly for qPCR analysis using specific primers and the iTaq Universal SYBR green mastermix (Bio-Rad). Primers for qPCR were designed using the Primer3 software44 ...
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bioRxiv - Neuroscience 2021Quote: ... ChAT) for 5 minutes or ECL Standard (β -actin) for 1 minute and then imaged using the ChemiDocMP Imaging System (BioRad). One sample from the Vehicle group was excluded from analysis for the BDNF western blot due to a transfer bubble over the band of interest for that sample ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Macrosep and Microsep Advance centrifugal devices 3 kDa MWCO and 0.2 μm syringe filters were purchased from Pall Corporation ...
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bioRxiv - Microbiology 2021Quote: ... or wet transferred using 0.5 M sodium phosphate transfer buffer (at a constant 1 Amp for an hour using a Bio-Rad apparatus). Membranes were blocked in 5% milk in PBS-T and probed with primary antibody (overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were incubated with the appropriate secondary antibodies for 1 h at room temperature and visualized using the Chemi-Doc XRS+ system (Bio-Rad). Primary antibodies used were anti-BCL-XL (abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Shooting: Dil-labeled bullets were shot through a 3.0 μm mesh 1 using a Helios Gene Gun system (Biorad, München, Germany) at 130 psi helium gas pressure.
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bioRxiv - Microbiology 2022Quote: ... added to a 15 μL reaction mixture containing 500 nM primers (sequences in Table 1) and iTaq Universal SYBR Green Supermix (Biorad, # 1725124) using a thermocycler (Mastercycler ep realplex2 ...
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bioRxiv - Evolutionary Biology 2022Quote: Plasmids pMAL-c4X-RecA and pMAL-c4X were electroporated with 1 pulse of 2.5 kV using a MicroPulse electroporator (Bio-rad, USA) into electrocompetent Lambda-lysogenized E ...
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bioRxiv - Genetics 2022Quote: ... The conidia were electroporated using 1 mm gap cuvettes (BIO-RAD Gene Pulser ®/ MicroPulser™ Cuvette) (Bio-rad, Hercules, California) at 1.5 kV ...
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bioRxiv - Genetics 2022Quote: ... Blots with HRP-linked secondary antibodies were additionally incubated for 1-5 minutes with Clarity or Clarity Max ECL Western Blotting substrates (Bio-Rad). Blots were then imaged on a ChemiDoc MP Imaging System and analyzed with Bio-Rad Image Lab software (v5.2.1).
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated at 37°C for 1 h with goat anti-mouse IgG StarBright Blue 520 or anti-rabbit IgG Starbright Blue 700 (Bio-Rad) secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 h at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... was used at a dilution of 1:1.000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:10.000) followed by a chemiluminescence reaction (Clarity ™ Western ECL Substrate, BIO-RAD).
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bioRxiv - Genetics 2019Quote: ... blocked in 5% non-fat dry milk, primary blotting using mouse anti-mNeonGreen (1:1000, Chromtek 32F6) or hFAB™ Rhodamine Anti-Actin (12004164 BioRad), and secondary blotting using 1:3000 anti-mouse HRP (NXA931 ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Molecular Biology 2020Quote: ... 1:1000)The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1hr and the detection was performed using the immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Products were separated by electrophoresis on a 0.7% agarose gel run in 1× TAE buffer at 55 V in the cold room and imaged on a ChemiDoc Touch instrument (Bio-Rad).