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Citations for Bio-Rad :
2451 - 2500 of 10000+ citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples containing 236 µg protein lysate in final 96 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006) were then prepared for subsequent reduction and alkylation for 25 min at room temperature by the simultaneous addition of 10X tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated by SDS-PAGE on 4–15% gradient Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Watford, Hertfordshire, UK), transferred to PVDF membranes (IPVH00010 ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µg of proteins from cell lysate or a quarter of the EV protein extract were loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad) in Tris-glycine buffer for electrophoresis at constant amperage (20 mA per gel ...
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bioRxiv - Molecular Biology 2024Quote: ... The apparent molecular weights (aMWs) of the protein complexes were calculated on the basis of the GFC protein standards (Bio-Rad, USA) as a control using the equation ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates were cleared by centrifugation (16000 g, 15 min) and protein concentrations determined by protein assay with Bradford dye reagent (Bio-Rad, 5000006). Proteins separated by SDS-PAGE without boiling due to the heat sensitivity of WDR62 [7] ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 µg protein was used per well of a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad). Protein denaturation was carried out using 8 M Urea in 1x Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was transferred to a clean tube and total protein concentration was measured with the DC protein assay (Bio-Rad #500-0112). 4X Laemmli Sample buffer (Bio-Rad #1610747 ...
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bioRxiv - Biophysics 2024Quote: ... we collected the supernatant in a new 1.5 ml reaction tube and determined the protein concentration of each sample by a Bradford protein assay (Biorad, Cat. No.: 5000006). For each sample ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 8–25 μg protein with 6x Laemmli buffer was loaded onto 4-15% or 4– 20% Mini-PROTEAN TGXTM Precast Protein Gels (Bio-Rad, 4561084) or 8 or 12% self- casted acrylamide/bisacrylamide (29:1 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 ug of protein samples were loaded per lane and electrophoresed on 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad, 4561025) and transferred to 0.2 μm nitrocellulose membrane using semi dry transfer system (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble lysate was normalized to 30 µg protein lysate in 15 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006). Proteins were then reduced and alkylated by the addition of 10x tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a nitrocellulose membrane (Bio-Rad) at 115V for 90 min ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined with Bradford reagent (Bio-Rad). 10% input was saved and equal amounts of protein were used for all Co-IPs ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... and the Fast Protein Liquid Chromatography (FPLC) system (BioRad). The proteins were eluted with filter sterilized buffer B (50 mM NaH2PO4 ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred to PVDF blotting membranes (Bio-Rad) in standard 25mM Tris-base ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were transferred to nitrocellulose membranes (Bio-Rad). Membranes were incubated in primary antibodies overnight at 4°C following blocking in 5% w/v nonfat dry milk in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (0.45 μm, BioRad) and blocked using LI-COR blocking buffer mixed 1:1 with PBS for 1 hr at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Precision plus protein Kaleidoscope molecular weight (MW) standards (BioRad) were separated in one lane of every gel ...
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bioRxiv - Cell Biology 2020Quote: ... protein was purified using the NGC Chromatography Systems (BioRad). In 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein content was measured by Bradford assay (Biorad). For each condition ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the Bradford reagent (Biorad). Immunoprecipitations were performed on 400 μg of protein extracts with the indicated antibodies and rotated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto a nitrocellulose membrane (Bio-Rad) and probed with primary antibodies following standard protocols ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (Bio-Rad) and blocked in 3% BSA in Tris Buffered Saline with 0.1% Tween 20 (TBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... in between Precision Plus Dual Color protein ladder (BioRad). After electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentrations were determined by Bradford assay (BioRad) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentrations were determined by Bradford assay (BioRad) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: Proteins were transferred on PVDF membrane (BioRad, Catalog: 1620177) by Bio-Rad tans-blot turbo transfer system at mixed weight transfer setting and incubated in 5% blotting grade blocker (BioRad Catalog ...
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bioRxiv - Microbiology 2021Quote: ... the proteins were transferred to nitrocellulose membranes (BioRad Laboratories) using the Trans-blot SD Semi-Dry system (BioRad Laboratories) ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined by Bradford assay (Biorad, USA). Further ...
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bioRxiv - Genomics 2020Quote: ... Following protein separation on precast SDS-PAGE gels (BioRad), proteins were detected using different primary antibodies (see Table S3) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using enhanced chemiluminesence reagent (Bio-Rad).
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bioRxiv - Pathology 2022Quote: ... using Bio-Rad Protein Assay Kit II (Bio-Rad), or DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were quantified with Quantity One (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined by Bradford assay (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to a nitrocellulose membrane (Bio-Rad) by wet transfer at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... bound proteins were eluted using sample buffer (BioRad, 1610747), complemented with 50 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purity was assessed on an SDS gel (BioRad). The enzymes were concentrated to 10-19.5 mg ml−1 with Amicon Ultra 10k and 30k MWCO centrifugal filters (Merck) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to 0.2 µm PVDF (Bio-Rad). Membranes were blocked with 5% milk in PBS-T and then blotted with a mouse anti-HTT (MAB2166 ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were transferred to a PVDF membrane (Bio-Rad) and blocked using 5% milk ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was determined using Bradford assay (Bio-Rad). 50 µL anti-GFP beads were added to 1 mg total protein diluted in a total volume of 600 µL RIPA buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using Bradford reagent (Bio-Rad). Lysates were boiled at 95 °C for 5 min and separated by SDS–PAGE electrophoresis using 8-16% Tris-Glycine gels (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Protein concentrations were determined with Bradford reagent (Bio-Rad). 15 µg of protein extract were loaded with 5x Laemmli buffer on Mini Protean TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Precision Plus Protein™ WesternC™ Standards (Bio-Rad) (2.5 μL ...
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bioRxiv - Immunology 2020Quote: ... Proteins were run on SDS-PAGE gels (Bio-Rad) and then transferred to nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... and stained with Flamingo fluorescent protein stain (Bio-Rad). Visualisation of the fluorescent signals was performed using the ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein quantification was done by Bradford assay (BioRad #5000201) or Pierce BCA protein assay kit (Thermo Scientific #23225 ...
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bioRxiv - Genomics 2021Quote: ... Protein content was measured with Bradford assay (Bio-Rad). 20 µg protein were denaturated at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Biorad, California, USA) using the Biorad TurboBlot™ system and blocked for one hour at room temperature in Odyssey® TBS-Blocking Buffer (Li-Cor BioSciences ...