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Citations for Bio-Rad :
2351 - 2400 of 10000+ citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins ≤ 250 kDa were separated by SDS-PAGE electrophoresis using 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and transferred to Immobilon-P PVDF Membrane (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was quantified by Qubit and 15ug protein was run on a 4-20% Mini-PROTEAN(R) TGX Stain-Free Protein gel (Bio-Rad). Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma ...
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bioRxiv - Developmental Biology 2023Quote: Whole cell and tissue protein was extracted using the Active Motif protein extraction Kit and quantified with the Bradford assay (Bio-Rad). Snap-frozen cardiac apical tissues were homogenized using Dounce tissue grinder (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was loaded for each sample (20 to 40 μg of protein lysates) and diluted with Laemmli Sample Buffer (Biorad, 1610747) and RIPA buffer in a final volume of 30 μL and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 10 µg (for FLN-DD experiment) of protein lysates for each treatment condition was fractionated in 4-15% Mini-Protean TGX protein gels (Bio-Rad) and transferred to nitrocellulose membrane using Bio-Rad Trans-Blot Turbo Transfer System with “Turbo Mixed MW” settings (constant 2.5A for 7 minutes) ...
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bioRxiv - Plant Biology 2023Quote: Protein samples of whole cell extracts (0.5 µg chlorophyll or 10 µg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: The protein of the lysates was then adjusted to 20 mg total protein using 4x Laemmli Sample Buffer (#1610747, BIO-RAD). The protein sample was denatured and run on 4-15% Mini-PROTEAN TGX Precast Protein Gels as per the manufacturer’s instructions (BIO-RAD) ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentrations in each tube were measured by Bradford assay using Quick Start Bradford protein assay kit (Bio-Rad). An equal amount of each sample (30-35 μl) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the concentration of the protein was determined either by SDS-PAGE or by Bradford assay using protein assay dye reagent (Bio-Rad) and bovine serum albumin (Carl Roth ...
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bioRxiv - Cell Biology 2024Quote: The protein extracts were separated by 10% SDS-PAGE and the proteins were transferred on the low fluorescence PVDF membrane (Bio-rad). The molecular weight of the proteins was assigned by Precision Plus Protein Dual Color Standards (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were visualized via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Precision Plus Protein Dual Color Standards (Bio-Rad) at volumes normalized to the initial hair mass ...
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and purity of extracted protein was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006) following manufacturer’ s protocol.
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bioRxiv - Immunology 2024Quote: ... 10 µL of each protein sample was then loaded and run on precast protein gels (4-20% Mini-PROTEAN® TGXTX, BioRad) before staining with Coomassie blue dye ...
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bioRxiv - Neuroscience 2024Quote: ... 20µg of protein was used for polyacrylamide gel electrophoresis (10% Mini-PROTEAN TGX Precast 15-well Protein Gels, Biorad, Hercules, CA) followed by transfer to a PVDF membrane (Merck KgaA ...
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bioRxiv - Neuroscience 2024Quote: ... equal amounts of protein (50 µg/lane) were loaded onto 4-20% gel electrophoresis of mini-protein TGX precast (Cat. #4561094, BIO-RAD) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were boiled at 95°C for 5 min and proteins were separated on 4–15% Mini-Protean TGX precast protein gel (Bio-Rad) and transferred onto hydrophobic polyvinylidene fluoride (0.45 μm PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... and Western blots were performed using standard molecular biology procedures Proteins were resolved by 4%–20% Criterion TGX protein gels (Bio-Rad) and transferred to PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 30 to 40 µg of protein were loaded into 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, 4561084), electrophoresed between 125 and 200 V for 30 min and wet-transferred to PVDF membranes (Millipore ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration in the homogenates was determined using a commercial assay kit (Bio-Rad Protein Assay, Bio-Rad, Hercules, CA, USA). Na+/K+-ATPase and H+-ATPase activities were expressed as µmol ADP mg protein-1 h-1 and carbonic anhydrase activity was expressed as µmol H+ mg protein-1 min-1.
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bioRxiv - Bioengineering 2024Quote: Protein samples were also isolated from iBMECs grown on transwell inserts in co-culture with iPericytes following the steps described above and 40 µg of total protein extract were separated by molecular weight using 10% Criterion TGX Precast Protein Gels (Bio-Rad). In this case transfers were carried out at 80V for 1.5h and membranes were also blocked for 1h before incubation with primary antibodies targeting tight junction proteins claudin-5 and ZO-1 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the Bio-Rad protein assay according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA). The proteins were then separated on a 10% polyacrylamide-SDS gel (90 µg of protein/lane ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg total protein from each sample was loaded onto 10% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) and separated for 1 h at 150 V ...
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bioRxiv - Molecular Biology 2024Quote: ... isolated mitochondria either bound to HA-beads or extracted from whole tissues homogenates were evaluated for protein concentration using the DC Protein Assay kit (Bio-Rad). Specific amounts of mitochondria based on assayed substrates were then resuspended in 20 μL of mitochondrial isolation buffer and plated onto a 96-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracted from the samples were quantified using RC DC Protein Assay Reagents Package (Bio-Rad Laboratories Ltd, Cat. No. 5000120,).
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bioRxiv - Neuroscience 2024Quote: ... SDS-PAGE-separated proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Immun-Blot® PVDF Membranes for Protein Blotting, Bio-Rad) with a transfer buffer (25 mM Tris-base ...
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bioRxiv - Cancer Biology 2024Quote: ... The same total protein amounts were loaded into each lane of 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Completed gels were transferred onto 0.2 um nitrocellulose membrane ...
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bioRxiv - Synthetic Biology 2024Quote: ... the samples were analyzed by western blotting using equal protein amounts of cell lysates separated by SDS–PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... and 20 μg of proteins were separated by SDS-PAGE using 4-20% Criterion TGX Stain-Free Protein Gels (5678094, Bio-Rad). Total protein stains were activated by UV excitation using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting supernatants were retained and protein concentrations were determined using the DC Protein Assay Kit II (Bio-Rad, Feldkirchen, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Samples (30 μg per sample) were loaded in TGX Stain-Free Protein Gels with one lane dedicated to a protein standard (Bio-Rad) and run at 100 V ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the PierceTM BCA protein assay kit and diluted with the 4x Laemmli sample buffer (BioRad, 1610747) before denaturation at 95°C for 5 minutes ...
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bioRxiv - Genetics 2024Quote: We loaded 10µL of protein sample per well on the Any kD Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad Laboratories). We ran the gel for 90 minutes at 100V in Tris/Glycine/SDS buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated by SDS-polyacrylamide gel electrophoresis (4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, BIO-RAD), transferred to nitrocellulose ...
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bioRxiv - Developmental Biology 2024Quote: ... and images of both the target proteins and the total protein were analyzed using ImageLab software (version 6.0.0, Bio-Rad, Hercules, USA) to determine the intensity per mm² of each band and lane ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected using horseradish-peroxidase conjugated anti-rabbit antibodies (BioRad) and detected with Western Lightning ECL reagents.
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody was HRP- conjugated goat anti-mouse antibody (Bio-Rad) used at a 1:5,000 dilution and incubated for 1 hr at room temperature ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... coli antibodies (BioRad, ON) or rabbit anti-Salmonella O antiserum group B antibodies (Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... α-StrepTag antibodies (Biorad). After transferring to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2024Quote: V5-antibody (Bio-Rad) was functionalised with a 20 kDa biotin-PEG-SVA linker (Laysan Bio ...
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bioRxiv - Immunology 2021Quote: ... Virus plaques were visualised by immunostaining with a rabbit polyclonal anti-NSP8 antibody (ABIN233792; Antibodies Online) and an HRP-conjugated anti-rabbit antibody (1706515; Bio-Rad). Plaques were quantified and IC50 values calculated using LabView or SigmaPlot software as previously described (28) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... assessment of protein concentration using Pierce™ BCA Protein Assay Kit (Thermo cat#23225) and addition of 4x Laemmli Sample buffer (Bio-Rad). 40ng of each sample was loaded onto Mini-PROTEAN GTX Precast Protein Gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... Molecular weight changes of the antigen were evaluated by reducing SDS-PAGE and protein bands were visualized with high sensitivity Flamingo Fluorescent Protein Gel Stain (1610491, Bio-Rad Laboratories). Intact antigen fractions were determined by digital imaging of gels followed by ImageJ analysis ...
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bioRxiv - Immunology 2021Quote: ... These tubes were then centrifuged at 300G for 5min at 4C and the supernatants were used for protein quantification using the DC protein quantification assay protocol (Bio-rad 5000112). After incubation at −80 °C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were centrifuged in v-bottom 96-well plates rather than microcentrifuge tubes and total protein concentration was determined using Protein Assay Dye (BioRad #500-0006) following standard protocols ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...