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Citations for Bio-Rad :
201 - 250 of 266 citations for Urotensin II Related Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to myosin II was estimated by a gel-shift assay (4–15% gradient gel, Biorad) and determined as >99% for both S1 and lever-arm-less S1 (Supplementary Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosomal DNA was separated in 1% agarose/0.5 × TBE on the CHEF-DR II Pulsed Field Electrophoresis Systems (Bio-Rad) as follows ...
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bioRxiv - Molecular Biology 2020Quote: Yeast genomic DNA was prepared in an agarose plug as described in the Instruction Manual (Bio-Rad, CHEF-DR II) with slight modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasma vitamin B12 concentrations were assessed using the BioRad Quantaphase II radio assay (Hercules, CA, USA;74. Plasma folate concentrations were also measured using the BioRad Quantaphase II radio assay (Hercules ...
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bioRxiv - Plant Biology 2023Quote: ... A quantitative real-time PCR (RT-qPCR) assay was performed on a LightCycler 480 II system using 2x SYBR Green qPCR master mix (BioRad). Maize GAPDH (Zm00001eb233140 ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-FLAG-HRP (clone D6W5B, CellSignalingTechnology #86861S, RRID:AB_2800094) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P, RRID:AB_609796) detection Abs were added for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Linearized plasmid was mixed with 250 μL of cells at 15°C in a 0.4 cm gap electroporation cuvette and transformed immediately by electroporation using a Gene Pulser II (Bio-Rad) set to 800V and 25 μF ...
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Zoology 2019Quote: ... Equal amounts of total protein per sample were separated by SDS gel electrophoresis on a 12.5% polyacrylamide gel [39] using a Mini-PROTEAN II Electrophoretic Cell (Bio-Rad, USA). Wet-transfer of the proteins from the gel to a polyvinylidene difluoride transfer membrane (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: Suprarenal aortas /AAA were isolated from Apoe−/−Il27ra+/− and Apoe−/−Il27ra−/− mice implanted with Angiotensin II pumps and homogenized in PureZol (Bio-Rad) with Ceramic Beads using Omni Bead Ruptor 24 ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
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bioRxiv - Microbiology 2020Quote: ... We covered the wells with a coverslip and we let them polymerize in a UV transilluminator (Bio-Rad Universal Hood II) for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The mixture was subjected to two consecutive electrical pulses of 850 V at 20 μF with a Gene Pulser II electroporator (Bio-Rad) and the cells were then seeded on a confluent monolayer of LR7 feeder cells in a 35mm dish ...
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bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
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bioRxiv - Developmental Biology 2021Quote: ... and 25 µg of the targeting vector was transfected into 1 × 107 of Nmt1-heterozygous cells11 by electroporation (240 V, 500 µF) with Gene Pulser II (Bio-Rad). One week later ...
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bioRxiv - Microbiology 2020Quote: ... baumannii cells using a Bio-Rad GenePulser II (1.8 kV, 25 μF, and 200 Ω) in a 1 mm electrode gap cuvette (Bio-Rad). Cells were recovered by resuspension in 1 mL of SOC medium and incubated at 37°C with shaking (200 rpm ...
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bioRxiv - Zoology 2020Quote: ... Telomeric repeats of sequence TTAGG were separated from restricted DNA with Pulsed Field Gel Electrophoresis (CHEF-DR II system by Bio-Rad) in a 0.8% agarose gel.
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bioRxiv - Molecular Biology 2019Quote: ... and an ATG initiation codon was co-electroporated with the pCAGGS-FLPe-puro into KH2 ESCs at 500V and 25 µF using a Gene Pulser II (Bio-Rad). Cells were selected with hygromycin (140 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with cold water and 10% glycerol and electroporated with the cat or kan PCR product using a GenePulser II instrument (2.5 kV, 0.2 cm gap cuvettes; Bio-Rad, Hercules, CA). Transformants were verified by colony PCR with primers approximately 200 bp up- and downstream of the gene of interest ...
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bioRxiv - Microbiology 2020Quote: ... Samples were assayed by Taqman multiplex real-time PCR using TaqMan Universal Master Mix II and a CFX384 Touch™ Real Time PCR detection system (BioRad). A standard curve was obtained using an in vitro transcript derived from a synthetic plasmid that contained all PCR targets ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reactions were analysed by agarose gel electrophoresis and image capture was performed on a Universal Hood II Gel Doc System (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: Hydrazine synthase α-subunit was purified by applying the 200 mM NaCl fraction from the Q-sepharose column on a 50 ml hydroxyapatite type II 40 μm (Bio-rad) column (XK 26/20 ...
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bioRxiv - Microbiology 2023Quote: ... The suspension was electroporated (310 V, 950 µF, 200 Ω, exponential) in a 2mm electroporation cuvette with a GenePulser II (BioRad). Electroporated parasites were immediately transferred into 5 mL complete culture containing 100 µL fresh ...
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... and chromosomal separation was conducted with the CHEF DR-II System and the CHEF Mapper XA System (Bio-Rad, Richmond CA) using different running and buffer conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Microbiology 2019Quote: ... the samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using the mini-PROTEAN II cell electrophoresis system (Bio-Rad, USA). The proteins were denatured in 2× loading buffer at 100°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...
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bioRxiv - Pathology 2019Quote: ... Vertical electrophoretic separation utilized 12.5% polyacrylamide gels and the following current parameters: 600 V/30 mA/100 W in an electrophoretic chamber (PROTEAN® II xi, Bio-Rad). The obtained gels were subjected to a standard colouring procedure with silver in the presence of formaldehyde as a regulator ...
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bioRxiv - Genomics 2019Quote: ... Protein concentration was measured using the RC DC (reducing agent and detergent compatible) Protein Assay Kit II (5000122, Bio-Rad; Hercules, CA). For qPCR experiments ...
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bioRxiv - Bioengineering 2019Quote: ... For equal loading the protein concentration of the supernatant was determined with BioRad DC protein assay kit II (BioRad#5000116, Cressier, Switzerland) and standard curve with Albumin (ThermoFisher #23210 ...
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bioRxiv - Genetics 2020Quote: ... agarose gel that was then stained with SYBR Green II (Tiandz, China) and imaged using a UV transilluminator from Syngene (Bio-Rad, USA).
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined in triplicate using a commercial colorimetric assay (Bio-Rad Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia). Nuclear and cytosolic fraction enrichment was confirmed by blotting the separated fractions against a nuclear (histone H3 ...
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bioRxiv - Biophysics 2022Quote: ... HbA (from transfusion)) was analyzed via High Performance Liquid Chromatography with a Bio-Rad Variant II Instrument (Bio-Rad, Montreal, QC, Canada) in the core clinical laboratory of University Hospitals Cleveland Medical Center (UHCMC) ...
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bioRxiv - Neuroscience 2023Quote: Proteins were separated on SDS-PAGE under denaturing conditions and transferred to nitrocellulose membrane using a MINI PROTEAN II™ system (Bio-Rad). Membranes were blocked with 5% milk powder/TBS-T and incubated with the primary antibodies overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cylc2-deficient mice were generated by electroporation of ribonucleoprotein (RNP) complexes into zygotes using a GenePulser II electroporation device (BioRad, Feldkirchen, Germany). For RNP formation ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified with the RC DC Protein assay Kit II (cat. No. 5000122; Bio-Rad Labortaories Inc, California, USA). After protein concentration assessment ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of cells were used for plasmid DNA extraction and equal volume of extracted DNA was resolved in agarose gel followed by ethidium bromide staining and visualized using Gel Doc XR+ UV Gel Documentation Molecular Imager Universal Hood II (Bio-Rad, USA).
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bioRxiv - Microbiology 2019Quote: ... The digested fragments were separated by PFGE (6.0 V/cm for 21 hours with a pulsing time linearly ramped from 2.2s to 63.8 s) using the CHEF DR-II apparatus (Bio-Rad Laboratories, Inc., Hercules, CA) on a 1% agarose gel ...
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bioRxiv - Genomics 2021Quote: ... qPCR experiments were conducted using a Roche LightCycler 480 II and Roche LightCycler 480 SYBR Green I Master SYBR-Green (Bio-Rad, Hercules, CA). Primers were designed to detect the KAN-MX resistance gene located on plasmids and genomic TUB1 (control ...
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bioRxiv - Neuroscience 2020Quote: ... Western blot data acquisition was conducted with LiCOR Odyssey Infrared imaging system (Model No. 9120) and Universal hood II (Bio-Rad, California, USA). Mean fluorescence values ...
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bioRxiv - Biochemistry 2020Quote: ... EV proteins were electrophoresed on a 12.5 % polyacrylamide gel (50V, 30 min) to obtain a single band (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France). The gel was stained with Coommassie Blue and the single band of proteins was excised and subjected to in-gel digestion with bovine trypsin (Roche Diagnostics GmbH ...
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bioRxiv - Pathology 2020Quote: ... LC3-II and p-mTOR were separated by electrophoresis and then transferred onto the nitrocellulose membranes (Bio-Rad; Trans-Blot Turbo Transfer System). After several washes with TBST buffer ...
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bioRxiv - Immunology 2020Quote: ... Thermo Fisher Scientific] and TaqMan PerfeCTa® qPCR FastMix® II mix (Quanta BioSciences) and detected by a CFX96 Real-time PCR Detection Machine (Bio-Rad). ΔΔCt was calculated to determine the relative expression normalized to β-actin.
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed by using a HiScript II one-step qRT-PCR SYBR green kit (Vazyme) on a CFX96 real-time PCR detection system (Bio-Rad Laboratories, Inc.). The following primers were used ...