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Citations for Bio-Rad :
201 - 250 of 10000+ citations for Plasma membrane calcium transporting ATPase 4 ATP2B4 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with horseradish peroxidase (HRP) conjugated goat anti-rabbit or mouse secondary antibody (Bio-Rad) for 1 h at room temperature ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... The membrane was incubated with a primary mouse anti-pig IL-18 antibody (diluted 1:500, Bio-Rad) or anti-β-actin antibody (diluted 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed 3 times in TBSt and then incubated with HRP secondary antibodies (anti-Mouse HRP, Biorad, 170-6516 ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with goat anti-rabbit IgG (H+L chains) HRP-conjugated secondary antibodies (Bio-Rad) and detected using SuperSignal West Pico ECL (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibody for 1 hour at room temperature (anti-mouse, 1:10.000, Bio-Rad). Antibody binding was detected by chemiluminescence (Clarity kit ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was incubated with 1:10,000 dilution of anti-mousehorseradish peroxidase (anti-mouse-HRP) antibody (Bio-Rad) at room temperature for 1 h and washed again three times in TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed thrice and incubated with appropriate secondary antibodies conjugated with HRP (Bio-rad; 1:3,000). Bands were detected by Western-Lightning Plus Enhanced Chemiluminescence (Perkin Elmer) ...
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bioRxiv - Immunology 2024Quote: ... Membranes were developed and observed by incubating with goat anti-rabbit IgG HRP-conjugated secondary antibody (Bio-Rad) and by using the ECL Western Blotting substrate as described previously 38.
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bioRxiv - Microbiology 2024Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (goat anti-rabbit HRP conjugate (BioRad, 170-5046, 1:5,000) and goat anti-guinea pig HRP conjugate (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight with the following primary antibodies: αWIPI2 1:3000 (mouse, Bio-Rad, Hercules, CA, MCA5780GA), αWIPI4/WDR45 1:2000 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with specific primary and secondary antibodies and visualized using SuperSignal West Pico PLUS (BioRad), a chemiluminescent substrate for horseradish peroxidase ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed in TBST and antibodies were detected by enhanced chemiluminescence (Clarity Western ECL substrate, Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to 0.2 μm PVDF membranes or nitrocellulose membranes (BioRad) by wet transfer using Tris Glycine transfer buffer (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... Nitrocellulose membranes were removed and replaced with PVDF membranes (BioRad #1620177) that had been activated in 100% methanol ...
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bioRxiv - Physiology 2023Quote: Plasma cytokines and chemokines were assessed using a magnetic bead panel (Bio-Rad, Cat. #171K1001M). Plasma samples were diluted 1:4 in assay buffer prior to running the assay according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Molecular Biology 2020Quote: ... Calcium levels were normalized to protein levels measured using the Bradford assay (Bio-Rad Laboratories, Hercules, CA, # 5000006).
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bioRxiv - Immunology 2022Quote: ... calcium in fetal bovine serum (FBS) was depleted by incubation with Chelex 100 resin (BioRad, Hercules, CA, USA) for 1 h at 4 C ...
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bioRxiv - Microbiology 2020Quote: ... and four electroporations were performed using 4×106 HeLa-H1 cells in a 4mm cuvette in 400μL of calcium and magnesium-free PBS using with 8μg of RNA in a Gene Pulser XCell (BioRad) set to 240V and 950uF ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted proteins were separated by NuPAGE 4-12% Bis-Tris gel in MES SDS running buffer and transferred to a PVDF membrane (Bio-Rad, 162-0263). Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty micrograms of protein were loaded on 4-15% gradient TGX Stain-Free™ Protein Gels and transferred to Immun-Blot® LF PVDF membrane (Bio-Rad). The membranes were blocked with 1X TBS with 0.05% Tween 20 and 5% skim milk powder for 2 hours at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The boiled proteins were separated on 4% to 15% SDS-PAGE gradient gels and transferred to LF PVDF membrane (Bio-Rad, Cat #1620260). The membranes were blocked in 5% skim milk in TBST (20 mM-Tris ...
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bioRxiv - Bioengineering 2022Quote: ... and an equivalent amount of protein lysate (12-15 µg) was electrophoresed on a 4-12% NuPAGE gradient gel and transferred onto low florescent polyvinylidene difluoride membranes (Bio-Rad, Cat: 1704274). Immunoblotting was performed with primary antibodies followed by secondary antibodies with the indicated dilutions ...
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bioRxiv - Physiology 2022Quote: ... ran on a 4-20% Criterion TGX Stain-Free Precast Gel and transferred to 0.2 µm PVDF membrane (Bio-Rad Laboratories, Inc, UK). Primary antibodies against FLAG (1:10,000 ...
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bioRxiv - Plant Biology 2020Quote: ... were centrifuged at 5000 x g for 15 min at 4°C and resolved by SDS-PAGE prior to transfer to a PVDF membrane (BioRad, Hercules, CA, USA). The membrane was then incubated with SnTox3 primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Same protein amounts were loaded onto 4-12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and incubated at 95°C for 3 min in SDS sample buffer before separation by SDS–PAGE on 4–15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules CA). Membranes were next blocked with 5% milk in TBS with 0.1% Tween 20 and incubated with primary antibodies and then HRP-coupled secondary antibodies in 5% milk or bovine serum albumin in TBS with 0.1% Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal quantity of the protein was resolved in a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred to an Immun-Blot PVDF Membrane (Bio-Rad) for antibody incubation ...
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bioRxiv - Physiology 2024Quote: ... Then we separated proteins into 4-15% gradient SDS-PAGE gels and transferred them to PVDF immuno-blot membranes (Bio-Rad, Hercules, CA). The primary antibodies S6 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Twenty micrograms of whole cell extracts were resolved on 4−12% SDS-polyacrylamide gels and transferred onto Ready gel blotting sandwiches immune-blot PVDF membranes (Biorad, Hercules, CA, USA) using a XCell II™ Blot Module (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... 20-40µg of total proteins were separated with SDS-PAGE in 4-15% polyacrylamide gel and then transferred to polyvinylidenedifluoride (PVDF) membranes (Bio-Rad, Hercules, CA). The membranes were blocked with 5% non-fat milk and immunoblotted with primary antibodies against CFTR (769 from the UNC distribution program ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with a horseradish-peroxidase (HRP)-conjugated secondary antibody and detected with ChemiDoc XRS+ System (Bio-rad). The antibodies were listed in Supplemental Table S12.
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bioRxiv - Genetics 2021Quote: ... Membranes were washed and probed with secondary antibody (1:5000, anti-Rabbit-HRP, 1706515; anti-Mouse-HRP 1706516, BioRad) in blocking solution ...
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bioRxiv - Physiology 2021Quote: ... The membranes were further washed in TBST buffer before adding secondary antibody (Bio-Rad, Goat-anti-rabbit, #170-6515) in 1% BSA (or 1% milk for SWELL1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with respective anti-mouse or anti-rabbit HRP-conjugated secondary antibody (1:1000, Bio-Rad) and visualized with a chemoluminescence kit (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were washed in TBS-Tween and incubated with the peroxidase-conjugated secondary antibody (Bio-Rad, Table 1) for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was subsequently washed and incubated in HRP-conjugated secondary antibody against the appropriate host (1:10,000, BioRad) for 1 hour RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody and detected with ChemiDoc XRS+ System (Bio-Rad). All western blots within a panel are from the same experiment and all blots were processed in parallel ...
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bioRxiv - Genomics 2024Quote: ... membranes were washed and incubated with HRP-conjugated secondary antibodies (goat anti-mouse or goat anti-rabbit, Bio-Rad). Protein expression was detected using an ECL detection kit (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit F(ab’)2 anti-mouse IgG conjugated to FITC (STAR9B, BioRad), and rabbit F(ab’)2 anti-rat IgG conjugated to FITC (STAR17B ...
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bioRxiv - Microbiology 2020Quote: ... 2 μl of anti-rabbit T lymphocytes fluorescently labeled with FITC (BioRad), and 2 μl of anti-rabbit IgM fluorescently labeled with FITC (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μl of anti-rabbit IgM fluorescently labeled with FITC (BioRad), and then incubated for 15 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The subsequent intracellular staining consisted of α-CD3e-FITC (CD3-12, Biorad) and α-CD79a-APC/eFluor780 (eBioscience) ...
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bioRxiv - Immunology 2020Quote: ... and incubated with either FITC-conjugated Sheep Anti-Human C3 (BioRad AHP031F) at 1:500 or AF488-conjugated Mouse Anti-C5b-9 (ae11 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and anti-F4/80 FITC (3:100, Bio-Rad Laboratories, Temse, Belgium). The single cells were washed with 1XPBS+1%FBS ...
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bioRxiv - Cell Biology 2022Quote: ... at 4 °C for overnight followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detection using Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... a double PARAFILM (Sigma)-layer flow chamber was incubated at 4°C overnight with 25ng/μL Digoxigenin Antibody (Bio-Rad) that adsorbed onto the polystyrene-covered lower surface ...
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bioRxiv - Neuroscience 2020Quote: ... nitrocellulose membrane (Bio-rad), sponges and cassettes were washed in water and soaked in transfer buffer prior to transfer ...
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bioRxiv - Physiology 2021Quote: ... PVDF membranes (BioRad®) and the SuperSignal West Pico Chemiluminescent Substrate Western blotting analysis system were from Thermo Fisher Scientific Inc® ...