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Citations for Bio-Rad :
151 - 200 of 10000+ citations for Plasma membrane calcium transporting ATPase 4 ATP2B4 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg per lane of total protein samples were separated on 4-12% Bolt Bis-Tris Plus Gels and blotted onto polyvinylidene difluoride membrane (Bio-Rad) using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... resolved on Novex 4–20% tris–glycine gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad). As a loading control ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of total protein were resolved in 4-20% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo transfer system (Bio-rad). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated on ExpressPlus™ PAGE Ready gels 4 - 20% (A2S, M42015) and transferred on the cellulose membrane (300 mA, 90 min) using a BioRad blotting device (BioRad, USA). After overnight blocking in 5% milk in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein samples (cell lysates and DRG fractionized membrane and cytosol) were size separated using 10% or 4-20% SDS-PAGE gels (Bio-Rad), transferred to Immun-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Cellular protein lysates were resolved on Criterion or 4%–20% Bio-Rad precast SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). Membranes were saturated and proteins were blotted with antibodies in 5% non-fat dry milk ...
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bioRxiv - Cell Biology 2020Quote: Transfected cells were lysed in RIPA buffer on day 4 and proteins extracted were separated by 10% - 15% SDS polyacrylamide gel and transferred to Nitrocellulose membranes (Bio-Rad) at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal masses of protein (5 ug – 40 ug) were separated by 4—15% of SDS/ PAGE and were transferred onto nitrocellulose membranes (Bio-Rad) for protein blot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were loaded to each lane on a 4-20% gradient SDS/PAGE gel and transferred to nitrocellulose membranes (Bio-Rad). Signals were developed with HRP-conjugated secondary antibodies and SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were resolved using 4–20% precast polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA). After blocking ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 µg protein was separated by electrophoresis on 4-12% polyacrylamide gels followed by transfer to nitrocellulose membranes (all gels/reagents from Bio-Rad). Membranes were then blocked (5% milk in TBS-Tween buffer ...
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bioRxiv - Immunology 2021Quote: ... Cell lysate proteins were resolved on a 4–12% gradient SDS-PAGE gel and transferred to Trans-Blot nitrocellulose membrane (Bio-Rad). Blots were incubated with polyclonal goat anti-mouse TLR2 Ab (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were incubated with the following primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µg proteins were analyzed through a 4-15% gradient SDS-PAGE before being transferred to the PVDF membrane with the TurboBlot system (Bio-Rad). The membrane was blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then transferred to a PVDF membrane at 400mA for 2 hours at 4°C in 1x Tris/Glycine buffer (1610771, Bio-Rad). Membranes were then blocked in casein blocking buffer (PI37528 ...
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bioRxiv - Neuroscience 2023Quote: ... Ten µg of total proteins quantified by PierceTM BCA protein assay kit (ThermoScientific) were loaded on 4-15% gradient SDS–PAGE gels and transferred to nitrocellulose membranes (Trans-Blot turbo, Bio-Rad). Immunoblotting of rodent cultures was detected by chemiluminescence with clarity Western ECL on ChemiDoc Touch system with Image Lab software (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved on 4–20% FastPAGE precast gels (Tsingke Biotechnology) and transferred to nitrocellulose membranes in a Trans-Blot Turbo machine (Bio-Rad). Membranes were blocked with 5% nonfat milk dissolved in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... The gel was run at 125 V for 3 to 4 hours and the RNA was then transferred to a Zeta-Probe GT membrane (Bio-Rad) by capillary action with 20 x SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Neuroscience 2023Quote: ... then 20 μg of protein was resolved on a 4-12% Tris gel and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo system (Bio-Rad) according to standard protocols ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were blocked with 4% bovine serum albumin (BSA) (Gemini Bio, 700-109P) dissolved in Tris-buffered saline (Bio-Rad #1706435) supplemented with 0.025% Triton X-100 (TBST) ...
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bioRxiv - Systems Biology 2023Quote: ... Whole-cell lysates were separated on an SDS-PAGE gradient gel (4–15%) and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad). p21 and Cdk2 were detected in 15 μg of whole-cell lysates ...
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bioRxiv - Cancer Biology 2023Quote: Protein samples were electrophoresed on home-made 4-16% Tris-glycine SDS-polyacrylamide gels and transferred to nitrocellulose membranes (0.45 µm, Bio-Rad, 1620115). Membranes were blocked in 5% non-fat milk ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... Protein was transferred to a methanol-activated PVDF membrane (IPFL85R, sigma) at 30 V overnight at 4°C (1703930, Bio-Rad). The membrane was then washed with 1x TBST with 2% SDS ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were incubated overnight at 4°C with primary antibodies and then probed with secondary antibodies before ECL development and imaging (Bio-Rad). Primary antibodies used for immunoblotting are as follows ...
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bioRxiv - Physiology 2021Quote: ... DA level was determined using the HPLC method (Plasma Catecholamine Kit, Bio-Rad, Germany) in combination with an Acclaim HPLC (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Nitrocellulose membranes (BioRad) were blocked with 5% w/V BSA in Tris-Buffer-Saline-Tween (TBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... PVDF membrane (BioRad). Other primary antibodies that were used are ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes (PVDF, BioRad) were developed using ECL reagent (Immobilon ...
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bioRxiv - Biochemistry 2023Quote: ... Zeta membrane (Biorad) was incubated in nuclease-free H2O while rocking for 10 min and air-dried for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes (BioRad, #1620177) were briefly reactivated in methanol and rinsed in water 3x ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-pig CD203a-FITC (clone PM18-7; Bio-Rad). Granulocytes were defined as 2B2+CD203a- ...
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bioRxiv - Biochemistry 2022Quote: ... calcium in fetal bovine serum was depleted by incubation with Chelex 100 resin (BioRad, Hercules, CA, USA) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... 1:4 & 1:8) and loaded in triplicates onto a nitrocellulose membrane assembled in a 96-well dot-blot apparatus (Biorad, Cat#1703938). The membrane was then stained with Ponceau (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Cell Biology 2021Quote: ... The cell lysates were separated by electrophoresis on pre-cast 4-15% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: 40 μg of protein lysate was resolved on NuPAGE Novex 4-12 % Bis-Tris gels and transferred onto nitrocellulose membranes (Bio-Rad system). Membranes were blocked with 5 % BSA in TBS-T (10 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Lysates were separated by NuPAGE 4- 12 % Bis-Tris Gels (Thermo Fischer Scientific) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer Systems (Bio-Rad Laboratories). Membranes were blocked with Odyssey Blocking Buffer (Li-Cor ...
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bioRxiv - Cell Biology 2023Quote: ... Protein from whole cell lysates and conditioned media were analyzed by running samples on SDS/polyacrylamide gel electrophoresis (4-15% Stain-free SDS-PAGE minigels) and transferred to PVDF membrane (Immun-Blot PVDF, Bio-Rad 1620177). Membranes were blocked in 3% BSA in Tris-buffered saline containing 1% Tween-20 (pH7.4) ...
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bioRxiv - Bioengineering 2022Quote: ... Fifteen micrograms total protein extracts were separated by 4–20 % stain-Free SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories). Membranes were blocked using Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated overnight at 4°C in blocking buffer containing 0.05 μg/ml anti-Mono-ADP-Ribose (Bio-Rad, AbD43647 #TZA020), which had been conjugated to BiCatcher2:HRP (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were loaded on 4–15% Criterion™ Tris-HCl Protein Gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad). Nonspecific binding sites were blocked for 1 h at room temperature in 5% nonfat milk in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C followed by horseradish peroxidase-conjugated secondary IgG antibody (Bio-Rad Laboratories) for 1h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were rinsed and incubated with the fluorescent secondary antibodies StarBright Blue 700 anti-mouse (1:5.000, BioRad) and StarBright Blue 700 anti-rabbit (1:5.000 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed in PBS-T or TBS-T and incubated with StarBright Blue secondary antibody (Bio-Rad) diluted in 5% milk in PBS-T for 30 min at room temperature in the dark and imaged using a Bio-Rad ChemiDoc imager.