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Citations for Bio-Rad :
201 - 250 of 1783 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: For the separation of myosin heavy chain isoforms a large format SDS-PAGE setup with integrated cooling core was used (Protean II xi, Bio-Rad Laboratories, Inc.). A 5% stacking gel ...
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bioRxiv - Immunology 2023Quote: ... Viral RNA genome was detected and quantified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on a BioRad real-time CFX96 detection system (BioRad, Hercules, United States). The target sequence for amplification was the viral RNA-dependent RNA polymerase [26].
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bioRxiv - Bioengineering 2024Quote: ... heavy chain (HC) and glutamine synthase (GS) selection marker genes was co-transfected in triplicate by electroporation (Gene Pulser Xcell™ Bio-Rad Laboratories) into exponential phase CHOK1SV GS-KO® host cells with piggyBac transposase mRNA according to Lonza proprietary methods ...
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bioRxiv - Zoology 2024Quote: ... Figure S1-S8) were measured using reverse transcription quantitative polymerase chain reaction (RT-qPCR) with Bio-Rad CFX96 (Bio-Rad, Hercules, CA, USA) in a reaction of 20 μ L ...
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bioRxiv - Molecular Biology 2024Quote: ... The real-time quantitative polymerase chain reaction (RT-qPCR) reactions were performed using a CFX96 touch real-time PCR system (Bio-Rad, CA, USA) and the RNA targets were identified utilizing the SYBR green fluorescent method ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with 30 ng of cDNA using the CFX Connect qPCR system (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was mixed with urea (0.5 M final concentration) before batch binding overnight at room temperature (RT) to nickel-charged IMAC resin (Profinity, BioRad) that had been previously equilibrated with the DARET Lysis Buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
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bioRxiv - Microbiology 2024Quote: ... Immune complexes were washed three times to exclude any non-specific binding followed by elution in Laemmli buffer (Biorad) containing 1 mM DTT and Western Blotting of eluates for HCoV-HKU1 or SARS-CoV-2 ΔPRRA spike.
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bioRxiv - Microbiology 2020Quote: ... vascular endothelial growth factor (VEGF) and IL-18 were measured on a Bio-Plex 200 instrument (Bio-Rad) using the Non-Human Primate Cytokine MILLIPLEX map 23-plex kit (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
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bioRxiv - Cell Biology 2023Quote: ... were removed from the eluted samples post-purification and quantified using quantitative Real Time polymerase chain reaction using the Bio-Rad CFX 96™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and Promega Plexor®HY Quantitation System (Promega Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time quantitative polymerase chain reactions (RT-qPCR) were performed in 10 μl reactions containing Power SYBER Green PCR master mix (Bio-Rad; Cat #: 172-5121), 5 ng of cDNA ...
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bioRxiv - Genetics 2021Quote: ... unspecific binding sites were blocked over night at 4°C with 3% milk powder (Marvel) in Tris Buffered Saline solution (BioRad) including 1% Tween 20 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... serially diluted rabbit serum (5-fold dilution starting with 1:80) were tested for binding to color-coded beads by Bio-plex (Biorad). The beads were coated with avi tagged C.1086 WT ...
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bioRxiv - Microbiology 2020Quote: Steady-state equilibrium binding of post-vaccination rabbit polyclonal serum was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant Spike proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
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bioRxiv - Immunology 2021Quote: Steady-state equilibrium binding of hCoV-2IG lots was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant SARS-CoV-2 RBD proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared with icecold lysis buffer and the protein concentration was measured by Coomassie binding (Bio-Rad). Equal amounts of protein were added to each well of a G-LISA plate and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: All binding assays were performed on a ProteOn XPR36 SPR Protein Interaction Array System (Bio-Rad Laboratories, Hercules, CA, USA). The instrument temperature was set at 25 °C for all kinetic analyses ...
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bioRxiv - Genetics 2023Quote: ... Differential binding was assessed by densitometry analysis of photostimulated luminescence (PSL) using Molecular Imager PharosFX System (Bio-Rad, #170-9450) and Quantity One 1-D analysis software (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-12 (10 ng/mL)] or transferred to a high-binding 96-well plate pre-coated with indicated amounts of control or TAG72 antigen (BioRad). Reagents and buffers for flow cytometry processing were pre-chilled on ice unless otherwise stated ...
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bioRxiv - Molecular Biology 2024Quote: ... binding was revealed with an enhanced chemiluminescence detection kit (1705062; Clarity Max Western ECL Substrate; BIORAD, Marnes-la-Coquette, France). Membranes were imaged on a ChemiDoc™ system (BIORAD ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies were incubated with the membrane before binding was visualised using EZ-ECL (Biological Industries, 20-500-120) and imaging on a ChemiDoc Imaging System (BioRad) [38].
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Microbiology 2024Quote: Gene expression analysis for host factors was performed using real-time RT-qPCR with iTaq SYBR Green Supermix (Biorad) in a CFX384 Touch RT-PCR detection system (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Physiology 2022Quote: ... real-time quantitative polymerase chain reaction (RT-qPCR) was executed with 13 ng cDNA using SSO advanced SYBR Green supermix (Bio-Rad Laboratories, Hercules, CA, USA) with the following settings ...
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bioRxiv - Immunology 2022Quote: Excess Eα peptide was removed with Bio-spin 30 kDa size exclusion columns (Bio-Rad Laboratories) equilibrated with PBS according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10, Biorad) and anti-2A peptide (3H4 ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Biochemistry 2021Quote: Primary screening for nanobody binding was achieved by a small-scale pulldown method in which Affi-gel 10 affinity resin (Bio-Rad) was bound to laforin and subsequently to each purified VHH clone34 ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Biochemistry 2023Quote: ... The slurry was gently stirred for 3 h to allow binding and then loaded into a glass Econo-Column (Bio-Rad). The column was washed with 10 Column Volumes (CV ...
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bioRxiv - Biochemistry 2023Quote: ... to saturate the N-terminal metal binding site,10 prior to buffer exchange using a centrifugal buffere exchange device (Bio-Spin, Bio-Rad) into 200 mM ammonium acetate supplemented with 2 x CMC of C10E5 ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Genetics 2024Quote: ... Bio-Rad was calibrated prior to measurements according to the manufacturer’s instructions with FAM dye and external well factor solution (Bio-Rad). All qPCR preparations were performed in a PCR hood (Fisher Scientific PCR Workstation ...
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bioRxiv - Immunology 2020Quote: ... Gene expression was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using SYBR Green PCR Master Mix (Bio-Rad Laboratories Inc., Hercules, CA, USA) using the gene-specific primers (Table 2 ...
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bioRxiv - Plant Biology 2020Quote: ... Peptide concentration was determined by a modified Lowry procedure using the DC Protein Assay (BioRad; Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Electrophoresis was used to separate peptides in a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Bio-Rad) with tris-tricine (Bio-Rad ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 10μl of EMSA binding reactions were loaded on 5% nondenaturing polyacrylamide Mini-PROTEAN TBE gels (Catalog #4565015, Bio-Rad Laboratories, Hercules, CA) for electrophoretic analyses ...
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bioRxiv - Cancer Biology 2020Quote: ... To reduce non-specific binding single cell suspensions were incubated for 5 min at 4°C with PBS/10% AB-serum (Bio-Rad, Germany), subsequently stained with fluorescence-labeled or biotinylated antibodies for 15 min at 4°C and washed once with PBS/2% FCS/0.01% NaN3 ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent, Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... A 1:1 Langmuir binding model was used to fit the data measured on a ProteOn™ XPR36 instrument (Bio-Rad Laboratories) using ProteOn™ Manager Software (Version 3.1.0.6 ...
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Physiology 2021Quote: ... chemokines and growth factors were quantitated by using a Bio-Plex Pro Mouse 23-plex Immunoassay and Bio-Plex Reader System (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). Peptides were labeled using 10-plex TMT reagents as described above and 4 μg of proteins were used for each TMT channel ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). 50 μg of each sample were set aside for TMT labeling ...