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Citations for Bio-Rad :
201 - 250 of 8299 citations for 7 Bromo 3 4 dihydro 1H benzo e 1 4 diazepine 2 5 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated 1h with HRP-conjugated secondary antibodies at 1:5,000 (Bio-Rad). After 4 more washes ...
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bioRxiv - Microbiology 2019Quote: ... for 1h at 1 mA/cm2 using a wet transfer system (Bio-Rad Laboratories). Blots were blocked and proved with 1° antibody in 5% milk (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
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bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Samples were boiled for 3 min and sonicated for 30 s prior to loading on 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad) and electrophoresis in Tris-glycine buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 3-4 μg of total RNA was reverse transcribed using the iScript advanced cDNA synthesis kit (Bio-Rad). Quantitative PCR of target genes (Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... The gel was run at 125 V for 3 to 4 hours and the RNA was then transferred to a Zeta-Probe GT membrane (Bio-Rad) by capillary action with 20 x SSC buffer (3 M NaCl ...
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... is used for calibration (Figure 1H Biorad #7318223 connected to tubing Picture 1C Tygon #R-3603) ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µg of protein was separated by SDS-PAGE using pre-cast TGX 4-15% gradient gels (BioRad). Protein was transferred to a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were run on 4-20% precast polyacrylamide gel (4-20% Mini-PROTEAN TGX Precast Protein Gels, BioRad) to separate them and transferred onto PVDF membranes using semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... SDS–PAGE was carried out using 4-15% or 4-20% precast polyacrylamide gels (Bio-Rad, 4561086 or 4561093) and run at a constant voltage of 80 V for 5 min followed by 120 V for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluent was isolated by centrifugation at 100 g for 4 min at 4°C (Bio-Rad 732-6204), buffer exchanged (Amicon Ultra 10 kDa MWCO UFC501024 ...
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bioRxiv - Cell Biology 2024Quote: ... with DTT and resolved on 4-15% 4-20% or 12% Mini-PROTEAN® TGX™ Precast Gels (Biorad) run in Tris/Glycine/SDS running buffer (Biorad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the samples were separated on 4-20% or 4-15% Mini-PROTEAN TGX Precast Protein Gels (BioRad Laboratories). The gel was transferred to a PVDF membrane using the iBlot transfer system (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... Total numbers of viable cells were determined after 2 and 4 days using the TC10 cell counter (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM borate pH 10 was prepared at 5 μM and loaded onto a 4-20% Mini-Protean TGX pre-cast gel (BioRad). 10 μL of each folding sample was subsequently loaded onto the same gel ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed with IP lysis buffer three time and boiled in 25 ul of 2X SDS-loading buffer for 5 minutes and loaded into 4-15% polyacrylamide gels (BioRad).
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bioRxiv - Neuroscience 2022Quote: ... heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl; BioRad) and transferred onto Immun-Blot PVDF 0.2 µm (BioRad ...
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bioRxiv - Cell Biology 2019Quote: ... a total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... pBAC-BoHV-4-A-revORF45HA and pBAC-BoHV-4-A-ΔORF45KanaGalK DNAs (5 μg) were electroporated in 600 μl DMEM high without serum (Biorad, Gene Pulser XCell ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed in 4 x 5 minutes in 0.2% TBS tween before incubating in Clarity™ Western ECL Substrate (BioRad) for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Immunology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Evolutionary Biology 2021Quote: ... gene expression was normalized with the housekeeping gene Act5c (primers: 5’ –gcgcccttactctttcacca-3’ and 5’ - atgtcacggacgatttcacg-3’. We performed the RT-qPCR analysis with SYBR Green (BioRad) or qPCRBIO SyGreen Blue Mix LO-ROX (PCR BIOSYSTEMS ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was 1:4 diluted in water and mixed with SYBR® Green (Bio-Rad) and the appropriate primers at 5µM ...
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bioRxiv - Biophysics 2020Quote: ... Color Development was obtained using the chromogenic substrate 4-chloro-1-naphthol (4CN, Bio-Rad) and H2O2.
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bioRxiv - Plant Biology 2023Quote: ... for 1 h at 4 °C and incubated with protein-G magnetic beads (Bio-rad) for 0.5 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha-Tubulin (rat, YL1/2, MCA77G, Bio-Rad, 1:2000 (Figure 5) or 1:10000 (other figures) ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were resolved on 4-12% Criterion XT Bis-Tris or 4-20% Mini-Protean TGX precast gels (BioRAD) and transferred to Immobilon-FL PVDF membranes (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µg of total protein per sample was loaded on NuPage 4-12% Bis-Tris Protein gels (Thermo Fischer Scientific) or 4–15% Mini-PROTEAN TGX StainFree Protein gels (Biorad) and run at 200 V as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... pH 5.3 (murine P-domains) or pH 7.3 (GII.4 Saga P-domain) at 4 °C via Micro Bio-Spin 6 columns (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Cells in monolayer were fixed in 4% PFA for 20 minutes at 4°C and blocked for 30 minutes in 10% donkey serum (BioRad) and 0,1% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... Typically 10-25 μg total protein was separated on reducing SDS-PAGE (4-15% or 4-20% gradient gels, BioRad). Proteins were transferred to nitrocellulose membranes ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... either on 7-12% Tris-glycine gels or gradient NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4-15%) (BioRad). List of antibodies and their working dilution used in this work are reported in (Table S4) ...
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bioRxiv - Plant Biology 2023Quote: ... either on 7–12% Tris-glycine gels or gradient NuPAGE 4–12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4–15%) (BioRad). A list of antibodies and their working dilution used in this work are reported in (Table S3) ...
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bioRxiv - Immunology 2023Quote: ... samples were run on either 4 to 15% or 4 to 20% Mini-PROTEAN TGX™ Tris-Glycine gels (BioRad) for 40-60 min at 160 V ...
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bioRxiv - Biochemistry 2023Quote: ... Protein in the supernatant was determined using Bradford assay and 30 µg loaded into 4-10% or 4-12% gradient SDS-PAGE gels (Mini-PROTEAN TGX Precast Gels, BioRad). Proteins were then transferred to 0.45 µm PVDF membranes (Immobilon-P ...