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Citations for Bio-Rad :
2401 - 2450 of 9277 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined in triplicate using a commercial colorimetric assay (Bio-Rad Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia). Nuclear and cytosolic fraction enrichment was confirmed by blotting the separated fractions against a nuclear (histone H3 ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Genomics 2021Quote: ... Both total and mitochondrial protein concentrations were determined using the Bradford method (Bio-Rad protein assay, Bio-Rad Laboratories GmbH, München, Germany). Protein extract volumes equivalent to 50–75 μg of total protein were concentrated into smaller volumes by lyophilizing the samples with a Savant Speed Vac Plus SC210A centrifugal evaporator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was transferred to a new tube and total protein concentration was determined by Bradford protein assay (BioRad, C500-0205). For each condition ...
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bioRxiv - Cancer Biology 2021Quote: Fifteen µg of protein per sample was separated on a BioRad Mini-protein 4-15% precast 12 well 20 µl gels (4561085, Bio-Rad, CA) and electrophoretically transferred to Odyssey Nitrocellulose membrane (P/N 926-31092 ...
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bioRxiv - Microbiology 2021Quote: ... protein pellets were resuspended in 100 µL of 6M urea in 50mM ammonium bicarbonate for DC® protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Neuroscience 2021Quote: ... An aliquot was taken to measure radioactivity and protein content using a liquid scintillation counter and a DC protein assay kit (Bio-rad, USA), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Immunology 2022Quote: ... The protein was concentrated using a 0.5 Centrifugal Filter Unit (Amicon) and then run on a Precast Protein Gel (Bio-Rad, 456-1103) (98 ...
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bioRxiv - Immunology 2022Quote: ... and the amount of protein was quantified by Bradford assay [31] using a colorimetric Protein Assay Dye (500-0006, Bio-Rad, USA) and Bovine Gama Globulin in known concentration for the standard curve ...
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bioRxiv - Immunology 2022Quote: ... and equal amounts of protein were loaded into a 4-15% gradient polyacrylamide gel (BioRad TGX™ Precast Midi Protein Gel #5671085) and proteins separated based on molecular weight using electrophoresis at 110V ...
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bioRxiv - Biochemistry 2022Quote: ... was loaded and electrophorized with a standard of 5□μl of Precision Plus Protein™ All Blue Prestained Protein Standards (BioRad #1610373). For electrophoresis ...
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bioRxiv - Plant Biology 2024Quote: ... The protein concentration of the filtered lysate was determined using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad; www.bio-rad.com) and bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2024Quote: Protein samples of whole cell extracts (5 μg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, US) and blotted onto nitrocellulose membranes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Proteins were separated on Any kD Mini-PROTEAN TGX Precast Protein Gels in a mini electrophoresis system (Bio-Rad Laboratories, California, USA). For molecular weight sizing ...
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bioRxiv - Molecular Biology 2024Quote: ... in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific. Proteins were transferred to a 0.2 µm nitrocellulose membrane by Trans-Blot Turbo™ Transfer system (Bio-Rad). Membranes were washed with TBS (50mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... Histone samples (4 μg protein/well) or whole cell lysates (see above, 40 μg protein/well) were separated on precast gels (4-20%, BioRad, CA, USA) and transferred to 0.2 μm nitrocellulose membranes (Turbo transfer pack ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Bioengineering 2024Quote: Protein expression was confirmed by SDS-PAGE analysis using 10% precast gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Cat# 4561033). The gels were run for 55-minutes at 150V and stained with G-250 Coomassie stain (Bio-Rad ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Equal amounts of protein (15ug) were loaded into each well of a Criterion XT Bis-Tris Protein Gel (Bio-Rad, Hercules, CA). Following gel electrophoresis ...
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bioRxiv - Microbiology 2022Quote: ... The reaction rate and total protein concentration (determined by the method of Bradford with the BioRad Protein Assay reagent; BioRad, Hercules, CA) were used to determine CAT activity ...
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bioRxiv - Synthetic Biology 2023Quote: ... prestained protein ladder were applied to SDS-PAGE using 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad, Hercules, CA, USA). Western blots were carried out in a Trans-Blot Turbo system using Trans-Blot Turbo Mini 0.2 μm Transfer-Packs (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was kept and the total protein concentration was determined by standard Bradford method (Bio-Rad Protein Assay Dye Reagent #5000006) using a BSA calibration curve (MP BIomedicals #160069 ...
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bioRxiv - Genetics 2023Quote: ... 50 µg of whole protein lysate was used for all immunoblots and resolved on 4-20% Mini-PROTEAN TGX Precast Protein gels (BioRad; Cat# 4561094). Protein samples were transferred to 0.2 µm PDVF membranes (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... and cell pellets were lysed in 1× Laemmli buffer before proteins were separated on 4-20% Mini- PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and transferred to a nitrocellulose membrane (Odyssey® nitrocellulose membrane ...
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bioRxiv - Biochemistry 2023Quote: ... ATP contents were expressed in nmol/mg protein (using the Bio-Rad Protein Assay Cat. #500-0006, Bio-Rad, Hercules, California, USA).
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 4°C at 20,160 x g for 10 minutes and supernatants were collected for protein measurement using the DC Protein Assay Kit (Bio-Rad, 5000111). Equal amounts of protein were loaded to run the gels (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified with the RC DC Protein assay Kit II (cat. No. 5000122; Bio-Rad Labortaories Inc, California, USA). After protein concentration assessment ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extracts were sonicated and cleared by centrifugation and protein concentration estimated using the Bio-Rad DC protein assay kit (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblotting of relevant phosphorylation-sites of proteins as well as total proteins was performed by standard immunoblotting techniques using commercially available equipment (BioRad Laboratories, USA). After the transfer of protein to polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, California, USA) and concentrated to approximately 100-200 μM.
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples containing 236 µg protein lysate in final 96 µL of Lysis Buffer using a Bradford protein concentration assay (Bio-Rad 5000006) were then prepared for subsequent reduction and alkylation for 25 min at room temperature by the simultaneous addition of 10X tris(2-carboxyethyl)phosphine hydrochloride (TCEP ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated by SDS-PAGE on 4–15% gradient Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Watford, Hertfordshire, UK), transferred to PVDF membranes (IPVH00010 ...
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bioRxiv - Biophysics 2024Quote: ... we collected the supernatant in a new 1.5 ml reaction tube and determined the protein concentration of each sample by a Bradford protein assay (Biorad, Cat. No.: 5000006). For each sample ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µg of proteins from cell lysate or a quarter of the EV protein extract were loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad) in Tris-glycine buffer for electrophoresis at constant amperage (20 mA per gel ...
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bioRxiv - Molecular Biology 2024Quote: ... The apparent molecular weights (aMWs) of the protein complexes were calculated on the basis of the GFC protein standards (Bio-Rad, USA) as a control using the equation ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates were cleared by centrifugation (16000 g, 15 min) and protein concentrations determined by protein assay with Bradford dye reagent (Bio-Rad, 5000006). Proteins separated by SDS-PAGE without boiling due to the heat sensitivity of WDR62 [7] ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a nitrocellulose membrane (Bio-Rad) at 115V for 90 min ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined with Bradford reagent (Bio-Rad). 10% input was saved and equal amounts of protein were used for all Co-IPs ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... and the Fast Protein Liquid Chromatography (FPLC) system (BioRad). The proteins were eluted with filter sterilized buffer B (50 mM NaH2PO4 ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred to PVDF blotting membranes (Bio-Rad) in standard 25mM Tris-base ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were transferred to nitrocellulose membranes (Bio-Rad). Membranes were incubated in primary antibodies overnight at 4°C following blocking in 5% w/v nonfat dry milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... Protein estimation was done using Bradford assay (Bio-Rad). Total extracted proteins (20 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (0.45 μm, BioRad) and blocked using LI-COR blocking buffer mixed 1:1 with PBS for 1 hr at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Precision plus protein Kaleidoscope molecular weight (MW) standards (BioRad) were separated in one lane of every gel ...