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Citations for Bio-Rad :
2351 - 2400 of 9277 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Whole cell and tissue protein was extracted using the Active Motif protein extraction Kit and quantified with the Bradford assay (Bio-Rad). Snap-frozen cardiac apical tissues were homogenized using Dounce tissue grinder (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was loaded for each sample (20 to 40 μg of protein lysates) and diluted with Laemmli Sample Buffer (Biorad, 1610747) and RIPA buffer in a final volume of 30 μL and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 10 µg (for FLN-DD experiment) of protein lysates for each treatment condition was fractionated in 4-15% Mini-Protean TGX protein gels (Bio-Rad) and transferred to nitrocellulose membrane using Bio-Rad Trans-Blot Turbo Transfer System with “Turbo Mixed MW” settings (constant 2.5A for 7 minutes) ...
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bioRxiv - Plant Biology 2023Quote: Protein samples of whole cell extracts (0.5 µg chlorophyll or 10 µg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: The protein of the lysates was then adjusted to 20 mg total protein using 4x Laemmli Sample Buffer (#1610747, BIO-RAD). The protein sample was denatured and run on 4-15% Mini-PROTEAN TGX Precast Protein Gels as per the manufacturer’s instructions (BIO-RAD) ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentrations in each tube were measured by Bradford assay using Quick Start Bradford protein assay kit (Bio-Rad). An equal amount of each sample (30-35 μl) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the concentration of the protein was determined either by SDS-PAGE or by Bradford assay using protein assay dye reagent (Bio-Rad) and bovine serum albumin (Carl Roth ...
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bioRxiv - Biophysics 2024Quote: ... total proteins and biotinylated proteins were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Microbiology 2024Quote: ... The same amounts of proteins were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat:4561096) or homemade 6-15% SDS-PAGE gels and transferred to a nitrocellulose membrane (Biorad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein Gels (#4561094, Bio-Rad) and transferred to 0.2 μM PVDF membranes by a Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Microbiology 2023Quote: ... 15 µg of membrane and soluble fraction were loaded after normalization by total protein quantification using the Bio-Rad Protein Assay (Bio-Rad) onto a 12% acrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: The protein extracts were separated by 10% SDS-PAGE and the proteins were transferred on the low fluorescence PVDF membrane (Bio-rad). The molecular weight of the proteins was assigned by Precision Plus Protein Dual Color Standards (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were visualized via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Precision Plus Protein Dual Color Standards (Bio-Rad) at volumes normalized to the initial hair mass ...
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and purity of extracted protein was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006) following manufacturer’ s protocol.
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bioRxiv - Immunology 2024Quote: ... 10 µL of each protein sample was then loaded and run on precast protein gels (4-20% Mini-PROTEAN® TGXTX, BioRad) before staining with Coomassie blue dye ...
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bioRxiv - Genetics 2021Quote: ... assessment of protein concentration using Pierce™ BCA Protein Assay Kit (Thermo cat#23225) and addition of 4x Laemmli Sample buffer (Bio-Rad). 40ng of each sample was loaded onto Mini-PROTEAN GTX Precast Protein Gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... Concentrations of the purified proteins were determined by Bradford Assay using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad 5000006) and calculating the ratio of the samples’ absorbance at 595 nm and 472 nm ...
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bioRxiv - Immunology 2021Quote: ... Molecular weight changes of the antigen were evaluated by reducing SDS-PAGE and protein bands were visualized with high sensitivity Flamingo Fluorescent Protein Gel Stain (1610491, Bio-Rad Laboratories). Intact antigen fractions were determined by digital imaging of gels followed by ImageJ analysis ...
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bioRxiv - Immunology 2021Quote: ... These tubes were then centrifuged at 300G for 5min at 4C and the supernatants were used for protein quantification using the DC protein quantification assay protocol (Bio-rad 5000112). After incubation at −80 °C for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured protein extracts were resolved using 4-9% SDS-PAGE (4–15% Mini-PROTEAN® TGX™ Precast Protein Gelscat# 4561083, Biorad) and then transferred to a nitrocellulose membrane.
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were centrifuged in v-bottom 96-well plates rather than microcentrifuge tubes and total protein concentration was determined using Protein Assay Dye (BioRad #500-0006) following standard protocols ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...
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bioRxiv - Bioengineering 2021Quote: ... the homogenate was centrifuged at 16,000g for 5 min and 5 µl of the supernatant was used for protein measurement in a microplate format utilizing the Lowry DC protein assay kit and γ-globulin for generating standard curve (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration of the supernatants was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-rad Laboratories, Inc., CA, USA); the absorbance was measured at 595 nm with a Thermo Scientific Multiscan® GO (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatants were transferred into a new set of tubes and protein concentration was determined using Bio-Rad DC™ protein assay kit (Bio-Rad). Western blot protocol is the same as described before ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad, 5000111) using a photospectrometer at 750 nm (Molecular Devices ...
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bioRxiv - Plant Biology 2021Quote: ... The total protein concentration of each sample was calculated by a Bradford assay following the Bio-Rad Protein Assay protocol (Bio-Rad, USA), and protein concentrations adjusted ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were analyzed on 10% Tris-Tricine SDS-PAGE system (38) with Precision plus protein standard (Bio-Rad 1610363) as a molecular weight marker ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble protein content was spectrophotometrically determined using bovine serum albumin as a standard (Bradford Protein Assay Kit; Bio-Rad, Hercules, CA).
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bioRxiv - Biochemistry 2022Quote: ... The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Biorad). Fractions containing high molecular weight species were pooled together and concentrated to <500 µL using a 100-kDa cutoff concentrator ...
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...
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bioRxiv - Biochemistry 2021Quote: ... Harvested cells were lysed in TNI lysis buffer and 10 μg of protein (as measured using Bio-Rad protein assay dye reagent) was aliquoted and precipitated via methanol/chloroform precipitation as described below ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... supernatants were transferred to new Eppendorf tubes and protein concentrations were then determined using a DC protein assay (Bio-Rad, Hercules, CA). 750 µg of protein was diluted in 4X Laemmli sample buffer (LSB ...
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bioRxiv - Molecular Biology 2019Quote: ... The IgG fraction was purified from hybridoma culture medium by Protein A chromatography on a Profinia™ protein purification system (Bio-Rad) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Protein concentration was measured using the RC DC (reducing agent and detergent compatible) Protein Assay Kit II (5000122, Bio-Rad; Hercules, CA). For qPCR experiments ...
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bioRxiv - Bioengineering 2019Quote: ... For equal loading the protein concentration of the supernatant was determined with BioRad DC protein assay kit II (BioRad#5000116, Cressier, Switzerland) and standard curve with Albumin (ThermoFisher #23210 ...
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bioRxiv - Plant Biology 2020Quote: ... This step was repeated two times and the protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, Munich, Germany) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... Protein concentration was determined in triplicate using a Bradford assay (Bio-Rad protein assay dye reagent concentrate, Bio-Rad Laboratories, Hercules, CA), against bovine serum albumin standards (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... followed a method adapted from our previous research.38 Protein concentrations were determined using the DC Protein Assay (Bio-Rad, Hercules, CA).
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bioRxiv - Plant Biology 2021Quote: ... Plant leaf protein samples (30-100 µg) were run on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Physiology 2019Quote: ... 25 µg of protein extract per sample was separated on 12% acrylamide gels (#5678044, Criterion™ TGX Stain-Free™ Protein Gel, Biorad) and blotted on PVDF membranes (Immobilon-FL IPFL00010 ...