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Citations for Bio-Rad :
2351 - 2400 of 10000+ citations for E3 Ubiquitin Protein Ligase RNF128 RNF128 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected using horseradish-peroxidase conjugated anti-rabbit antibodies (BioRad) and detected with Western Lightning ECL reagents.
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bioRxiv - Plant Biology 2024Quote: ... α-StrepTag antibodies (Biorad). After transferring to nitrocellulose membrane ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... coli antibodies (BioRad, ON) or rabbit anti-Salmonella O antiserum group B antibodies (Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Virus plaques were visualised by immunostaining with a rabbit polyclonal anti-NSP8 antibody (ABIN233792; Antibodies Online) and an HRP-conjugated anti-rabbit antibody (1706515; Bio-Rad). Plaques were quantified and IC50 values calculated using LabView or SigmaPlot software as previously described (28) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... assessment of protein concentration using Pierce™ BCA Protein Assay Kit (Thermo cat#23225) and addition of 4x Laemmli Sample buffer (Bio-Rad). 40ng of each sample was loaded onto Mini-PROTEAN GTX Precast Protein Gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... Concentrations of the purified proteins were determined by Bradford Assay using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad 5000006) and calculating the ratio of the samples’ absorbance at 595 nm and 472 nm ...
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bioRxiv - Immunology 2021Quote: ... Molecular weight changes of the antigen were evaluated by reducing SDS-PAGE and protein bands were visualized with high sensitivity Flamingo Fluorescent Protein Gel Stain (1610491, Bio-Rad Laboratories). Intact antigen fractions were determined by digital imaging of gels followed by ImageJ analysis ...
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bioRxiv - Immunology 2021Quote: ... These tubes were then centrifuged at 300G for 5min at 4C and the supernatants were used for protein quantification using the DC protein quantification assay protocol (Bio-rad 5000112). After incubation at −80 °C for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured protein extracts were resolved using 4-9% SDS-PAGE (4–15% Mini-PROTEAN® TGX™ Precast Protein Gelscat# 4561083, Biorad) and then transferred to a nitrocellulose membrane.
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were centrifuged in v-bottom 96-well plates rather than microcentrifuge tubes and total protein concentration was determined using Protein Assay Dye (BioRad #500-0006) following standard protocols ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...
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bioRxiv - Bioengineering 2021Quote: ... the homogenate was centrifuged at 16,000g for 5 min and 5 µl of the supernatant was used for protein measurement in a microplate format utilizing the Lowry DC protein assay kit and γ-globulin for generating standard curve (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration of the supernatants was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-rad Laboratories, Inc., CA, USA); the absorbance was measured at 595 nm with a Thermo Scientific Multiscan® GO (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatants were transferred into a new set of tubes and protein concentration was determined using Bio-Rad DC™ protein assay kit (Bio-Rad). Western blot protocol is the same as described before ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad, 5000111) using a photospectrometer at 750 nm (Molecular Devices ...
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bioRxiv - Plant Biology 2021Quote: ... The total protein concentration of each sample was calculated by a Bradford assay following the Bio-Rad Protein Assay protocol (Bio-Rad, USA), and protein concentrations adjusted ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were analyzed on 10% Tris-Tricine SDS-PAGE system (38) with Precision plus protein standard (Bio-Rad 1610363) as a molecular weight marker ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble protein content was spectrophotometrically determined using bovine serum albumin as a standard (Bradford Protein Assay Kit; Bio-Rad, Hercules, CA).
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bioRxiv - Biochemistry 2022Quote: ... The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Biorad). Fractions containing high molecular weight species were pooled together and concentrated to <500 µL using a 100-kDa cutoff concentrator ...
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...
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bioRxiv - Biochemistry 2021Quote: ... Harvested cells were lysed in TNI lysis buffer and 10 μg of protein (as measured using Bio-Rad protein assay dye reagent) was aliquoted and precipitated via methanol/chloroform precipitation as described below ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... supernatants were transferred to new Eppendorf tubes and protein concentrations were then determined using a DC protein assay (Bio-Rad, Hercules, CA). 750 µg of protein was diluted in 4X Laemmli sample buffer (LSB ...
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bioRxiv - Molecular Biology 2019Quote: ... The IgG fraction was purified from hybridoma culture medium by Protein A chromatography on a Profinia™ protein purification system (Bio-Rad) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Protein concentration was measured using the RC DC (reducing agent and detergent compatible) Protein Assay Kit II (5000122, Bio-Rad; Hercules, CA). For qPCR experiments ...
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bioRxiv - Bioengineering 2019Quote: ... For equal loading the protein concentration of the supernatant was determined with BioRad DC protein assay kit II (BioRad#5000116, Cressier, Switzerland) and standard curve with Albumin (ThermoFisher #23210 ...
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bioRxiv - Plant Biology 2020Quote: ... This step was repeated two times and the protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, Munich, Germany) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... Protein concentration was determined in triplicate using a Bradford assay (Bio-Rad protein assay dye reagent concentrate, Bio-Rad Laboratories, Hercules, CA), against bovine serum albumin standards (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... followed a method adapted from our previous research.38 Protein concentrations were determined using the DC Protein Assay (Bio-Rad, Hercules, CA).
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bioRxiv - Plant Biology 2021Quote: ... Plant leaf protein samples (30-100 µg) were run on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Physiology 2019Quote: ... 25 µg of protein extract per sample was separated on 12% acrylamide gels (#5678044, Criterion™ TGX Stain-Free™ Protein Gel, Biorad) and blotted on PVDF membranes (Immobilon-FL IPFL00010 ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined in triplicate using a commercial colorimetric assay (Bio-Rad Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia). Nuclear and cytosolic fraction enrichment was confirmed by blotting the separated fractions against a nuclear (histone H3 ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Genomics 2021Quote: ... Both total and mitochondrial protein concentrations were determined using the Bradford method (Bio-Rad protein assay, Bio-Rad Laboratories GmbH, München, Germany). Protein extract volumes equivalent to 50–75 μg of total protein were concentrated into smaller volumes by lyophilizing the samples with a Savant Speed Vac Plus SC210A centrifugal evaporator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was transferred to a new tube and total protein concentration was determined by Bradford protein assay (BioRad, C500-0205). For each condition ...
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bioRxiv - Cancer Biology 2021Quote: Fifteen µg of protein per sample was separated on a BioRad Mini-protein 4-15% precast 12 well 20 µl gels (4561085, Bio-Rad, CA) and electrophoretically transferred to Odyssey Nitrocellulose membrane (P/N 926-31092 ...
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bioRxiv - Microbiology 2021Quote: ... protein pellets were resuspended in 100 µL of 6M urea in 50mM ammonium bicarbonate for DC® protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Neuroscience 2021Quote: ... An aliquot was taken to measure radioactivity and protein content using a liquid scintillation counter and a DC protein assay kit (Bio-rad, USA), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Immunology 2022Quote: ... The protein was concentrated using a 0.5 Centrifugal Filter Unit (Amicon) and then run on a Precast Protein Gel (Bio-Rad, 456-1103) (98 ...
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bioRxiv - Immunology 2022Quote: ... and the amount of protein was quantified by Bradford assay [31] using a colorimetric Protein Assay Dye (500-0006, Bio-Rad, USA) and Bovine Gama Globulin in known concentration for the standard curve ...
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bioRxiv - Immunology 2022Quote: ... and equal amounts of protein were loaded into a 4-15% gradient polyacrylamide gel (BioRad TGX™ Precast Midi Protein Gel #5671085) and proteins separated based on molecular weight using electrophoresis at 110V ...
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bioRxiv - Biochemistry 2022Quote: ... was loaded and electrophorized with a standard of 5□μl of Precision Plus Protein™ All Blue Prestained Protein Standards (BioRad #1610373). For electrophoresis ...