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Citations for Bio-Rad :
2251 - 2300 of 10000+ citations for E3 Ubiquitin Protein Ligase RNF128 RNF128 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: 40-60μg of protein was electrophoresed through 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hertfordshire, UK) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB1 and DdvT were detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The purified proteins were separated by SDS/4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA), then transferred to an Immobilon-FL PVDF membrane (Merck Millipore Ltd. ...
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bioRxiv - Bioengineering 2020Quote: ... Equal amounts of protein lysates (10-20 μg total protein) were subjected to 4–20% SDS-PAGE (Bio-Rad, Hercules, CA). Western analysis was carried out with primary antibodies against phosphorylated Smad1/5 (p-Smad1/5) ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of the protein samples were separated on 4-20% SDS-PAGE (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) at 20 mA and 250 V as the limiting voltage.
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bioRxiv - Biochemistry 2020Quote: ... the protein concentration was determined using the BioRad DC microplate assay protocol (BioRad DC Protein Assay Kit, BioRad, Hercules, California #5000112). Protein Standards and samples were run on NuPAGE 10% Bis-Tris Gels (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad®) for separation (200 V ...
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bioRxiv - Neuroscience 2021Quote: ... and sonicated (twice; 70 % power for 30 sec, Sonopuls, Bandelin) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... cAMP content in TCA extracts was determined by radioimmunoassay (RIA) (83) and normalized to protein [(determined using a dyebinding protein assay (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... Additional one-ml samples were used for total protein determination using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). β-galactosidase assays were performed as described previously (38) ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μl of brain homogenate from each sample was analyzed for protein concentration using BioRad protein assay reagent (BioRad, Hercules, CA). 1 μl of 0.2 M perchloric acid per sample was added to the remaining homogenate and was centrifuged at 10,000 rpm for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were normalised after Bradford protein quantification and resolved on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad), transferred to nitrocellulose and blocked in 5% milk in PBS-T (PBS + 0.05% Tween 20) ...
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bioRxiv - Physiology 2020Quote: ... 15 μg of protein was separated on a 10% Criterion® TGX™ Precast Protein Gel (BioRad Laboratories, 56671034, Hercules, CA) and transferred to a nitrocellulose membrane using a Trans-Blot TurboTransfer System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2019Quote: Protein concentrations in the EVs and WCP samples were determined by Quick StartTM Bradford protein microplate standard assay (Bio-Rad, USA). For protein separation ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled for 5 min and the proteins were separated in 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-RAD) and electroblotted to nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration of the EV was determined using the Quick Start™ Bradford protein assay according to manufacturer’s guidelines (BioRad, UK). In short ...
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bioRxiv - Neuroscience 2020Quote: ... 40 μg protein of N13 cell lysates was loaded to 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BioRad) and transferred to nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Transfer Kit (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl of the protein extracts were loaded on a 4-12% gradient 26 well Criterion XT Bis-Tris Protein gel (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... steroid concentrations or their ratios were normalized to the total protein content of the incubations as determined in the supernatant after cell lysis using the DC Protein Assay (Bio-Rad).
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and the protein concentration was measured using a DC protein assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of protein (5 μg ...
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bioRxiv - Biochemistry 2021Quote: ... 10 uL of the protein loading mixture was loaded into the wells of either 12% mini-PROTEAN TGX precast protein gels (Bio-rad) or 12.5% SuperSep Phos-tag precast gels (Wako Pure Chemical Industries) ...
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bioRxiv - Biophysics 2019Quote: ... Gradients were manually fractionated into 200 μL fractions and protein concentrations were determined by the Bradford assay (Protein Assay Dye Reagent Concentrate 5×, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a new tube and protein concentration was determined using Protein Assay Dye Reagent Concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 10µg of protein per sample was loaded into 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and run at 105V for 90 minutes ...
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bioRxiv - Pathology 2022Quote: ... 2-5%β-mercapto-ethanol for 10min at 70°C and then migrated with protein ladder (Precision plus protein all blue standards, Bio-Rad) in Bio-Rad gels (Criterion TGX ...
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bioRxiv - Immunology 2019Quote: ... Samples were then centrifuged for 30 min and protein concentration was measured using the DC Protein Assay according to standardized protocols (Bio-Rad). Protein concentration in supernatants was normalized to 1 mg/mL and frozen at −80°C until assay ...
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bioRxiv - Synthetic Biology 2020Quote: ... The boiled samples were centrifuged at 14,000 rpm for 10 minutes and 20 μg of total protein per sample was then loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA) and ran at 140 V ...
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bioRxiv - Immunology 2019Quote: Total protein concentration in bronchoalveolar lavage fluid (BALF) were measured by the Bradford Assay using Protein Assay Dye Reagent Concentrate (Bio-Rad) according to the manufacturer’s protocol ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifugation at 14000 rpm for 8min at 4° C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). The proteins were run on 7%-15% SDS-PAGE gels (50μg per lane ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted 1:20 in 1X PBS and protein concentration was quantified by the DC protein assay with BSA as the standard (BioRad®). Lysates were normalized to 5 μg/μL in 2X loading buffer (Lysis buffer supplemented with 5 mM beta-mercaptoethanol and Coomassie blue) ...
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bioRxiv - Molecular Biology 2019Quote: ... The results were normalized to total protein content in the corresponding cell RIPA lysates (Quick Start Bradford Protein Assay, Bio-Rad). The ELISA detects both the 163S and 157S chemerin.
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bioRxiv - Molecular Biology 2020Quote: ... Glycerol was added to the supernatants (final concentration: 10%) and protein concentrations were adjusted using Bio-Rad protein assay (Bio-Rad). The protein samples were diluted in 6x loading buffer (9.8% glycerol ...
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bioRxiv - Molecular Biology 2021Quote: ... The amount of protein in the extracts was assessed by the Bradford method using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad). Protein electrophoresis in 10% or 12% PAA gels with SDS in 1x Laemmli buffer was carried out ...
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bioRxiv - Molecular Biology 2021Quote: Self-association of GST-SP proteins was determined by native gel electrophoresis using Mini-PROTEAN TGX 4–20% precast polyacrylamide protein gels (Bio-Rad). Purified wild-type or mutant GST-SP (5 μg ...
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bioRxiv - Microbiology 2019Quote: ... we prepared a mixture of 25 µL each of Protein A and Protein G SureBeads Magnetic Beads (Bio-Rad, Hercules, CA) and immobilized 1–2 µg of primary antibody on the beads by rotation at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Precision Plus Protein All Blue and Kaleidoscope Pre-stained Protein Standards molecular weight ladders were purchased from Bio-Rad (Hercules, CA). Vectashield mounting media with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... Austria).52 The luciferase activity data was normalised to protein content measured by DC Protein determination kit (Bio-Rad Laboratories, Inc., USA) and SCO effect was expressed as a fold-change over untreated cells ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was used to quantify the total protein concentration in the sample using the DC protein assay (Bio-Rad) a BSA standard curve [34] ...
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bioRxiv - Immunology 2021Quote: ... Enzymatic biotinylation with BirA ligase was performed and protein was purified by size-exclusion fast protein liquid chromatography using a NGC Quest 10 Chromatography System (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... Protein sample loading buffer was added to cell lysates and proteins were separated on SDS-PAGE (BioRad Criterion TGX Precast Gel). Antibodies used for immunoblotting are listed in Supp ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Bioengineering 2020Quote: ... The concentration of each fraction of proteins was determined by SDS-PAGE and quantitatively by Bradford protein assay (BIO-RAD, USA).
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bioRxiv - Genetics 2021Quote: ... Protein concentration in each sample was measured through Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, USA) and spectrophotometry at 595 nm (BMG Labtech ...
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bioRxiv - Neuroscience 2021Quote: ... 10-50 μg of protein was loaded onto 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and transferred to polyvinylidene difluoride (PVDF ...