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Citations for Bio-Rad :
2201 - 2250 of 3153 citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... which was inoculated at 30°C for 1 hour and then photographed using a UV transilluminator (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... Amplification products were resolved in 1% (w/v) agarose gel and analysed with GelDoc XR+ System (Bio-Rad Laboratories). Primers and probes are listed in Table S1.
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were detected with anti-mouse HRP-conjugated secondary antibodies (1:5000; Bio-Rad, Hercules, CA, USA), and the signal was developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used: rabbit anti-mouse collagen-IV (Bio-Rad Company, cat.# 2150-1470, 1:300), rat anti-mouse CD45 (BD Pharmingen ...
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bioRxiv - Genomics 2019Quote: ... All blots were visualized using a goat anti-rabbit IgG:alkaline phosphatase fusion protein at 1:3,000 dilution in conjunction with BCIP/NBT color development (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... First-strand cDNA synthesis was performed from 1 μg isolated RNA template using iScript RT supermix (Bio-Rad #1708841). PCR products were amplified using a CFX96 Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Biophysics 2019Quote: ... Gel precursor solutions were prepared with 6% v/v acrylamide and 0.2% v/v bisacrylamide (30% acrylamide/bisacrylamide stock 29:1, BioRad), 2 mM LAP photoinitiator (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... RNA (1 μg) was reverse transcribed in 20 μl reactions using the BioRad iScript cDNA Synthesis Kit (BioRad Laboratories) with 1:1 mixture of oligo-dT and random hexamers for 30 min at 42°C ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA was synthesized from 1 μg total RNA with an iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). A CFX 96 Connect™ system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the Amylose resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were washed in TBS-Tween and incubated with the peroxidase-conjugated secondary antibody (Bio-Rad, Table 1) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Molecular Biology 2021Quote: ... the Netherlands) and blocked for 1 hour at room temperature using Odyssey blocking buffer (LI-COR biosciences, Bio-Rad) in a 1:1 dilution with tris-buffered saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of mRNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). RNA was hydrolyzed and re-suspended in nuclease free water ...
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad). ImageLab (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR analysis was performed using the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-rabbit Igg (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6515,) were used as secondary antibodies ...
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bioRxiv - Genomics 2022Quote: ... membranes were rinsed threetimes in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Plant Biology 2022Quote: ... USA (17) and a 1:3000 dilution of goat anti-rabbit IgG horseradish peroxidase (HRP) conjugate secondary antibody (Biorad). Chemiluminescence was detected using WesternBright ECL (Advansta ...
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bioRxiv - Microbiology 2022Quote: ... A 15% SDS-Tricine gel was made by mixing: 2.5 mL 30% acrylamide/Bis solution 37.5:1 (BioRad #1610158), 1.25 mL gel buffer (1.5 M Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was subsequently washed and incubated in HRP-conjugated secondary antibody against the appropriate host (1:10,000, BioRad) for 1 hour RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... together with 2.5 µl of single (1 µl assay + 1.5 µl NF water) or multiplexed (0.5 µl of each assay) PrimePCR Probe Assays (Bio-Rad) as follows with single or multiplex reactions grouped in parentheses and formatted as Gene/UniqueAssayID ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 μ of total RNA using iScript gDNA clear cDNA synthesis kit (Bio-Rad, 1725035) with random primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli and plasmid DNA were electroporated in 1 mm cuvettes at 1.8 kV using the MicroPulser electroporator (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... We synthesized the first strand cDNA with 1 µg of total RNA using iScriptTM Reverse Transcription Supermix (BIO-RAD) followed by quantitative PCR with CFX96 Real-Time System (BIO-RAD ...
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bioRxiv - Cancer Biology 2024Quote: Cells were first embedded in gel plugs with approximately 1 million cells per gel plug (BioRad no. 170-3592). The high molecular weight genomic DNA was extracted and purified using the Bionano Genomics SP kit.
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bioRxiv - Cell Biology 2024Quote: Polyacrylamide gel electrophoresis was carried out using 10 % gels (acrylamide/bis-acrylamide ratio 37.5:1) or any kDa precast gels (Biorad) and a MiniProtean (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysates and purified supernatants were separated on an SDS-PAGE and analyzed through immunoblotting against vinculin as a loading control (1:2,000, MGA465GA, BioRad) or CHIKV E1 glycoprotein (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad). 20 μL of the end products were diluted by adding 180 μL nuclease-free water and stored at -20 °C until further analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated goat anti-rabbit IgG (1:5000; 1706515, Bio-Rad) at RT for 1 h followed by chemiluminescent detection with ECL Substrate (1705060 ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription (RT) was performed on 1 µg RNA with the iScript™ Reverse Transcription Supermix (Bio-Rad 1708841) for 5 min at 25°C ...
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bioRxiv - Genomics 2024Quote: ... F43E2.6 siRNA and miR-1 Ct values were assayed using a CF96 Real-Time PCR Detection System (Bio-Rad). Ct values were averaged across three technical replicates for each of 3 biological replicates.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using candidate primers (Integrated DNA Technologies; Table 1) and an iScript cDNA Synthesis Kit (Bio-Rad). Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... and goat anti-rabbit (Cat. # 170-6515) antibodies were used at 1:2000 for immunoblotting and purchased from Biorad.
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bioRxiv - Genomics 2024Quote: ... samples were embedded in a thin polyacrylamide film by inverting them onto a GelSlick-coated microscope slide with a droplet of 4% acrylamide solution (4% v/v 20:1 acrylamide:bis-acrylamide [BioRad, 1610144] with 0.15% v/v TEMED [Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Immunology 2024Quote: ... qPCR was then performed with a 1:10 dilution of cDNA using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 6 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: 0.2 – 1 mL (sufficient to cover the sample) of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) with 0.2% Tween20 and 0.1 mg/mL proteinase K (Merck ...
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bioRxiv - Immunology 2024Quote: ... Amplicons were resolved in a 1% agarose gel and visualized using the ChemiDoc MP Imaging System (Biorad, Hercules, CA). When specified ...
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bioRxiv - Neuroscience 2023Quote: ... each primary was detected via HRP conjugated Goat α-Rabbit secondary antibody (1:4000, Bio-Rad, product 170-6515) after incubation with Clarity ECL substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked for 1hr in PBS with 1mg/ml and 0.1% triton and then stained overnight with anti-alpha Tubulin antibody (1:100, MCA78G, BioRad). Cells were then washed ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:10,000, 52200336 and 52200341, Kirkegaard & Perry Laboratories) and detected by enhanced chemiluminescence using a Chemidoc (BioRad) gel imaging system ...