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Citations for Bio-Rad :
2001 - 2050 of 3153 citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was obtained by retrotranscription of 1 μg of total mRNA with iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Reactions were then analysed by 20% denaturing polyacrylamide gel electrophoresis (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Systems Biology 2021Quote: ... Denatured cellular extracts equivalent to 1/120 OD600 were loaded onto a 7.5% precast Mini-Protean TGX (BioRad) and resolved by SDS-PAGE ...
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bioRxiv - Physiology 2020Quote: ... exposed to UV for 1 min and total protein patterns were visualized using ChemiDoc MP Imager (Bio-Rad). The stain-free gel contains a trihalo compound which reacts with proteins during separation ...
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bioRxiv - Molecular Biology 2020Quote: First-strand cDNA was synthesized using 1 μg of RNA with the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Immunohistochemistry was performed on paraffin sections (5 μm) using antibody F4-80 (Bio-rad, France; diluted 1:100). Sections were incubated overnight at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of total RNA was used to synthesize cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad). Real-time RT-PCR was performed using gene specific primers (Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... and an extension at 1 °C in a Bio-RAD T100™ Thermal Cycler (Bio-RAD, Hercules, CA). PCR amplicons from each reaction were purified using a QIAquick PCR Purification kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.2-1 µg of RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad). Real-time PCR assay was conducted using the SYBR Premix ExTaq II (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription and qPCR were performed using the iTaq Universal SYBR Green 1-Step Reaction Mix (BioRad 1725151) on a Bio-Rad CFX Connect Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
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bioRxiv - Plant Biology 2022Quote: ... 10-day-old seedlings true leaves of Arabidopsis lines were bombarded with 1 nm gold particles (Bio-Rad) coated with plasmid DNA ...
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bioRxiv - Genomics 2022Quote: ... Obtained cDNA was diluted 1:10 and subjected to qPCR analysis in triplicates using iQ™ SuperMix (BioRad). Viral mRNA expression was measured with HIV-1 gag primer probe mix (Supplementary Table 9 ...
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bioRxiv - Microbiology 2022Quote: ... for 1 hr and then counted using either an easyCyte 12HT or a ZE5 flow cytometer (Bio-Rad). Prochlorococcus cell sizes were normalized to 2 µm beads (Polysciences) ...
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bioRxiv - Biophysics 2022Quote: ... and 1 mM EDTA in 99.9% deuterium oxide using a spin desalting column (Micro Bio-Spin 6, BioRad). 15N-HSQC spectra were measured every 40 min for 106 hours at 25 °C on Bruker 600 MHz NMR spectrometer ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated from 1 µg of RNA using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad). Real-time quantitative PCR was performed on a StepOnePlus real-time PCT Machine (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein pellet was allowed to dry on air and was dissolved in 1 × Laemmli sample buffer (Bio-rad). Samples were heated at 70 °C for 10 min and 20 μg of protein for each sample was loaded onto a 1.5 mm 12% polyacrylamide gel ...
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bioRxiv - Cell Biology 2019Quote: ... 5 min) and incubated for 1 h at room temperature with goat anti-rabbit IgG (H+L) (BioRad). Membranes were washed and signal was detected using the ChemiDoc XRS+ (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... HRP detection was performed by adding 1 mL Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
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bioRxiv - Biophysics 2020Quote: ... a kind gift from Julian Eaton-Rye) with anti-rabbit HRP-conjugated goat antibody (1:3000, Bio-Rad) as a secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:15000 in blocking buffer) followed by a goat anti-rabbit-HRP conjugate (170-6515, Bio-Rad, Hercules ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) (Bio-Rad, Hercules, CA) or anti-mouse IgG-HRP conjugate (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal total protein concentration were heated at 90°C in 1 × loading buffer (Bio-Rad 1610747) for ten minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: Western Blot samples of unbound fractions and IP were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Neuroscience 2022Quote: ... horseradish peroxidase (HRP) conjugated secondary antibodies (1: 5000) and signal detected using ClarityTM Western ECL Substrate solution (BioRad). Blots were visualised on a ChermiDOC XRS+ system (BioRad ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Immunoreactive bands were visualized using horseradish peroxidase-conjugated goat anti-rabbit IgG (Bio-Rad #170-6515; 1:3000), followed by ECL detection (GE Life Sciences) ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (Biorad ...
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bioRxiv - Biochemistry 2019Quote: Complementary DNA was synthesized from 1 μg of purified RNA using the iScript cDNA Synthesis Kit (BioRad, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Both PK-treated and untreated samples were then mixed 1:2 with 4X Laemmli sample buffer (Bio-Rad) containing DTT ...
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bioRxiv - Microbiology 2019Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF) and protein concentration was determined using the DC protein assay (Bio-Rad). Whole cell lysates were resolved by SDS-PAGE in 4-20% gradient Mini-PROTEAN TGX gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at RT and detected using Clarity or Clarity Max Western ECL Blotting Substrate (Bio-Rad) and imaged (Amersham AI600) ...
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bioRxiv - Cell Biology 2019Quote: ... Immune complexes were detected with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (1:5,000 dilution; Bio-Rad 1706515).
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bioRxiv - Developmental Biology 2019Quote: ... cells were incubated for 1 h on ice and finally measured using the S3e™ Cell Sorter (BioRad) apparatus ...
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bioRxiv - Evolutionary Biology 2020Quote: ... NCBI accession DQ278441.1) (Table 1) were used for quantitative PCR using the iQ SYBR Green Supermix (BioRad, Canada) and the following cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 1 mg of RNA using the iScript cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Blots were subsequently incubated with 1:2000 dilution of goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at RT ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... The membrane was incubated with a primary mouse anti-pig IL-18 antibody (diluted 1:500, Bio-Rad) or anti-β-actin antibody (diluted 1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 h at rtp with 5% (w/v) non-fat milk block (Bio-Rad) in 1x TBS (in mM ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 30 min at room temperature with α-CD3 (1 µg/mL; clone UCHT1; Bio-Rad). CD8+ T cells were negatively selected from blood and TDL using a CD8+ T Cell Enrichment Kit (stemCell Technologies) ...
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bioRxiv - Immunology 2019Quote: ... was detected with goat anti-rabbit AF 647 and sheep anti-TGN46 primary antibody (Biorad, AHP500, 1:100) was detected with donkey anti-goat AF 647 (1:500).
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bioRxiv - Immunology 2019Quote: ... purified PR3 mutant proteins were loaded (1 µg/lane) onto 12% Tris-HCl gels from BioRad (Hercules, CA). The SDS gel electrophoresis was performed at 180 volts for 35 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... then 3 washes in 1% milk were performed prior to imaging the blot with ECL (BioRad Clarity #1705060). Membrane was stripped using the GM Biosciences One Minute Advance Stripping buffer #GM6031 ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated with Goat Anti-Mouse IgG (Heavy + Light)-HRP conjugate (170-6516, 1:1,000; Bio-Rad) overnight and following PBS-T washing detected using the LAS-500 Imaging System (General Electric).
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bioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription of 1 μg of RNA was carried out with iScript cDNA Synthesis Kit (1708891, Bio-Rad) using an extended transcription step of three hours at 42°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4970) overnight at 4 °C and the HRP-conjugated secondary antibodies (BIO-RAD, Cat. No. 1706515, 1:1000) for 1 h at room temperature ...