Labshake search
Citations for Bio-Rad :
2201 - 2250 of 2503 citations for 5 chloro 2 hydroxyacetophenone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... concentration of total aerobic GNB and of MDR-GNB were determined by plating serial dilutions (pure, 10−2, 10−4) of initial faeces or EA sample onto Drigalski agar (Bio-Rad) with or without 1mg/L cefotaxime ...
-
bioRxiv - Immunology 2021Quote: ... Lysates were finally adjusted to a final concentration of 2 µg/µl with 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA) containing 10% β-Mercaptoethanol.
-
bioRxiv - Microbiology 2019Quote: ... 250 µl packed cells were combined with 250 µl of 1 × Cytomix in a 2 mm transfection cuvette (Bio-Rad Laboratories). The precipitated and resuspended DNA was added to the cell suspension in the cuvette ...
-
bioRxiv - Biochemistry 2019Quote: ... MenD mutants were generated using the pYUB28b-menD construct and oligonucleotide primers (Supplementary Table 2, Integrated DNA Technologies) with iProofTM high-fidelity DNA polymerase (Bio-Rad). The PCR products were then treated with DpnI and ligated using T4 DNA ligase (Roche) ...
-
bioRxiv - Developmental Biology 2019Quote: ... and incubated with 1:200 rabbit-anti NF-B p65 polyclonal antibody (Pierce) (2 h) and 1:5000 Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) (1 h) ...
-
bioRxiv - Microbiology 2019Quote: ... was determined for different genes (primers are listed in Table 2) by quantitative PCR (qPCR) using iTaq SYBR Green (Bio-Rad) with 400 pg of cDNA and 0.25 μM each primer in 10 μL reactions ...
-
bioRxiv - Bioengineering 2019Quote: ... Cells were then washed three times with DI water and stained with 2 ml hematoxylin (Bio-Rad Laboratories, Hercules, CA, USA) for 15 min at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... We then performed quantitative PCR (qPCR) using 2 ng of cDNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
-
bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
-
bioRxiv - Biophysics 2021Quote: ... The mixture was diluted to ~30 mL with sample buffer and stirred at 4°C for 30 min prior removal of C12E8 by adding 4 g of Bio-Beads SM-2 (Bio-Rad) in stages of 0.5 ...
-
bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... and indicated primers (2 μL) were added in the PCR reactions (ddPCR Supermix for Probes (No dUTP) mix from Bio-Rad) in a total of 25 μL ...
-
bioRxiv - Biophysics 2020Quote: ... Inputs (2 μl) and elutions (10 μl) samples were analyzed by 4-15% SDS-PAGE (MINI-PROTEAN TGX stain-free, Bio-Rad) and staining with Quick Coomassie (Generon ...
-
bioRxiv - Neuroscience 2021Quote: ... Incubation with appropriate HRP-conjugated secondary antibodies (Thermo) was for 2 hours at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD). Densitometric analysis was performed using ImageJ software (NIH).
-
bioRxiv - Molecular Biology 2020Quote: ... for 2 h at room temperature and a HRP-conjugated goat anti-rabbit secondary antibody (Bio-Rad 1706515; 1:3000 dilution) for 1 h at room temperature ...
-
bioRxiv - Synthetic Biology 2021Quote: ... reactions were analyzed by urea-PAGE 2 μL of the reaction was mixed with 12 μL of TBE-Urea (Bio-Rad) loading dye and boiled for 10 minutes at 100 °C ...
-
bioRxiv - Plant Biology 2020Quote: ... PCR products were resolved electrophoretically on ethidium bromide stained-2% agarose gel immersed in 1× TAE and visualized with Biorad Gel Doc XR instrument (Biorad, USA).
-
bioRxiv - Microbiology 2020Quote: ... and placed on ice for 2 min before loading on a 10% denaturing urea polyacrylamide gel in TBE buffer (Bio-Rad) next to an RNA marker (low range ssRNA ladder ...
-
bioRxiv - Microbiology 2021Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 μg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
-
bioRxiv - Neuroscience 2020Quote: ... 2 μl of brain homogenate from each sample was analyzed for protein concentration using BioRad protein assay reagent (BioRad, Hercules, CA). 1 μl of 0.2 M perchloric acid per sample was added to the remaining homogenate and was centrifuged at 10,000 rpm for 10 minutes at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... The DDM/CHS detergent was then removed by overnight incubation with Bio-Beads SM-2 (15 mg per 1 ml protein/detergent/amphipols mixture, Bio-Rad) at 4 °C ...
-
bioRxiv - Biophysics 2021Quote: ... DIV15-30 neurons were transfected 2-6 days before imaging via biolistic gene transfer (160 psi, Helios gene gun, Bio-Rad), as described in a detailed video protocol (Woods and Zito 2008) ...
-
bioRxiv - Developmental Biology 2022Quote: ... anti-SARS CoV-2 Spike Neutralizing antibody (SAD-S35, ACRO, USA) or anti-Human IgG Kappa (STAR 127, Bio-Rad, USA) antibody ...
-
bioRxiv - Cell Biology 2022Quote: ... 25-50 follicles were collected per sample and boiled in sample buffer (Laemmli Sample Buffer, Bio-Rad + 2-mercaptoethanol, Bio-Rad). Samples were run on 4-15% gradient gels (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... 25-50 follicles were collected per sample and boiled in sample buffer (Laemmli Sample Buffer, Bio-Rad + 2-mercaptoethanol, Bio-Rad). Samples were run on 4-15% gradient gels (Bio-Rad ...
-
bioRxiv - Biophysics 2022Quote: ... detergent was removed from the sample by the gradual addition of 400 mg/mL SM-2 polystyrene Bio-Beads (Bio-Rad) over the course of four hours ...
-
bioRxiv - Microbiology 2022Quote: ... 0.02% (v/v) H2O2) were prepared and mixed followed by incubation for 2 min for chemiluminescence detection with ChemiDocTM MP System (BIO-RAD).
-
bioRxiv - Genetics 2022Quote: ... The entire sample was loaded on 1.5 mm 8% SDS-PAGE gel and ran at 85 volts for 2 hours and transferred to PDVF membrane via semidry transfer (Bio-Rad) at 19 V for 2 hours and 30 minutes ...
-
bioRxiv - Genetics 2022Quote: ... The samples were boiled at 65°C for 10 minutes and then loaded on 10% SDS-PAGE gel and ran at 90 volts for 2 hours and transferred to a PDVF membrane via semidry transfer (Bio-Rad) at 13 V for 3 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.2 μm nitrocellulose membranes were prewet for 10 minutes in TBS then secured into the Bio-Dot Microfiltration apparatus (Bio-Rad). Wells were rehydrated once with TBS ...
-
bioRxiv - Neuroscience 2022Quote: ... was performed for 2 h at room temperature and protein bands were visualized using the Clarity Western ECL Substrate (BIO-RAD).
-
bioRxiv - Cell Biology 2022Quote: ... The PCR product was separated in a 2% agarose gel and imaged by ChemiDoc™ Touch Imaging System (Bio-rad Laboratories).
-
bioRxiv - Plant Biology 2021Quote: ... and 2 μg of the RNA was employed to synthesize cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). Quantitative RT- qPCR analysis was performed on a Bio-Rad iQ5 real-time PCR instrument with the iQ SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad) were added and incubated for 16 hours at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad) were added and incubated for 16 hours at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... Primers (Supplementary Table 2) were designed using Primer3 Plus (24, 25) to quantify transcripts using Universal SYBR Green Supermix (Bio-Rad) using a Quantstudio 6 Flex (Applied Biosystems ...
-
ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The RNA sample was denatured by incubating it at 70°C for 2 min in S1000TM or C1000TM Thermal Cycler PCR machine (Bio-Rad). The denatured RNA was quickly transferred on ice ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was diluted 1:4 in nuclease free water and each cDNA was combined with segment specific primers (Supplementary Table 2) and Precision Melt Supermix (Bio-Rad) and analyzed by qPCR in a CFX384 Touch real-time PCR detection system (Bio-Rad ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 2 hours at room temperature with HRP-conjugated goat anti-rabbit or goat anti-mouse secondary antibodies (Bio-Rad), diluted 1:5000 in 5% nonfat milk in TBS-T ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Reaction mixtures were incubated for 2 hours at 25 °C and quenched by adding 10 μL 4x Laemmli Sample buffer (Bio-Rad). Samples were boiled at 95 °C for 5 min and each 8 μL were loaded onto 12% SDS-PAGE gels ...
-
bioRxiv - Plant Biology 2022Quote: ... 500 nM concentration of each PCR primer and 2 μL of 15x diluted cDNA templates using CFX96 real-time PCR system (Bio-Rad) as described (Gayomba et al. ...
-
bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... The reactions were assembled isothermally on a PTC-225 Peltier Thermal Cycler (MJ Research) or a Tetrad 2 Peltier thermal cycler (Bio-Rad) at a suitable growth temperature (e.g ...
-
bioRxiv - Developmental Biology 2019Quote: ... total RNA from Astyanax embryos of various stages (2-24 hpf) was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad) and amplified using the following primers:
-
bioRxiv - Zoology 2020Quote: ... Samples were incubated in a 1:1 (w/v) ratio of 2× sample buffer (0.2M DTT, BioRad Laemmli Sample Buffer #1610737) at room temperature for 20 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... cells were infected with SINV at an MOI of 2 and pictures were taken 6 hours post-infection using ZOE fluorescent cell imager (Bio-Rad). Proteins were collected with lysis buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Immunology 2019Quote: ... to nitrocellulose membranes using a constant voltage of 100 V for 2 h using the BioRad Midi PROTEAN Western blotting module according to the manufacturer’s instructions (BioRad, CA, USA). The membranes were blocked with blocking solution [Tris-buffered saline (TBS ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
-
bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
-
bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).