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Citations for Bio-Rad :
2101 - 2150 of 2503 citations for 5 chloro 2 hydroxyacetophenone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hr and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 µg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Biophysics 2022Quote: Detergent removal and generation of proteoliposomes was achieved using polystyrene beads (Bio-Beads in SM-2 Adsorbent Media, BioRad, Inc.). The protocol was adapted and refined from 20 ...
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bioRxiv - Systems Biology 2023Quote: ... and total protein abundance was normalized to 2 μg using the Detergentcompatible Protein Assay (Biorad, Hercules, CA, USA, Cat. # 5000111). 2 μg α-RelA antibody (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 mg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the nitrocellulose membrane was incubated with ECL reagent SignalFire for 1-2 min and imaged using a ChemiDoc (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Cancer Biology 2023Quote: ... qPCR duplicates were carried out using 2 µL of diluted cDNA (1 into 100) in a 20 µL-reaction using Sso Fast EvaGreen reagent (BioRad) following supplier’s instructions on a StepOne Plus™ Real-Time PCR system (Applied BioSystems) ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a 0.2 µm or a low-fluorescence 0.45 µm polyvinylidene difluoride (PVDF) transfer membrane using a Trans-Blot Turbo (Bio-Rad). Following antibody incubation ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hour and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated from ∼2 ng of total RNA from each sample using iScript Reverse Transcription Supermix for qRT-PCR (170-8891; BioRad) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... 390 μl of cells were mixed with 10 μg of in vitro transcribed RNA and added to a 2 mm electroporation cuvette (BioRad). BHK-21 cells were electroporated with 1 pulse at 1.2 kV ...
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bioRxiv - Immunology 2023Quote: ... Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad, Cat Nos. 12015406 and 12014773), and Bio-Plex Pro Human IgA detection antibody (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Duplicates of samples and plasmid standards were amplified by using the following protocol ...
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bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Cell Biology 2023Quote: ... then the pellet was resuspended in 212 µL of Laemmli sample buffer with 2-mercaptoethanol added (Bio-Rad, Hercules, CA). To neutralize the residual TCA ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was determined using the 2−ΔΔCT method129 as part of the CFX Maestro for Mac 1.0 software package (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The mixture was allowed to stand at room temperature in the dark for 30 min before the detergent was removed by successive addition of BioBeads SM-2 (BioRad). Nanodiscs were concentrated using Vivaspin® 500 centrifugal filters (10 kDa MWCO PES membrane).
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were resuspended by addition of ice-cold PBS supplemented with 2% FBS and counted using a TC-20 cell counter (BioRad). 2.5-5x105 cells were harvested ...
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bioRxiv - Microbiology 2024Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Microbiology 2024Quote: ... DD-HA-BFD2Δeif1.2 were subjected to electrophoresis using 10% polyacrylamide SDS-PAGE gels and transferred onto 0.45-µm nitrocellulose membranes (Bio-Rad) with wet/tank transfer system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 500μL of sample was dialyzed (20 kDa cutoff) in 250 mL Buffer A with 0.5 g Biobeads SM-2 (Bio-Rad) for 16-18 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The ddPCR reaction mixture consisted of 12.5 µl of a 2× ddPCR Supermix for Probes no dUTP (1863024; Bio-Rad), 1.25 µl of each 20× primer-probe mix (Table 2) ...
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bioRxiv - Immunology 2024Quote: ... incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min and visualized using the chemiluminescent setting on a ChemiDoc XRS+ (Biorad).
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bioRxiv - Immunology 2024Quote: ... and dialyzed overnight at 4 °C into 4 L of Experimental Buffer (25 mM Tris, 100 mM NaCl, pH 7.4) with 2 g/L Chelex 100 resin (Bio-Rad) to chelate residual transition metal ions ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed using 2 µL of genomic DNA in SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, 172-5271) and primers for a 529-base pair repetitive element of T ...
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bioRxiv - Molecular Biology 2024Quote: ... Gene-specific cDNA synthesis was carried out with 2 µg of mtRNA using the iScript Select cDNA Synthesis Kit (BioRad) and oligo 2278 (see Supplementary Table ST2) ...
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bioRxiv - Immunology 2024Quote: ... and incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min before visualization using the chemiluminescent setting on the ChemiDoc XRS+ (Biorad).
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bioRxiv - Cell Biology 2020Quote: ... The protein samples were mixed with LDS sample loading buffer at 4X concentration followed by addition of beta-mercaptoethanol (5%) (Bio-Rad, Hercules, CA). The resultant mixture was denatured at 95°C for 9 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X) on QuantStudio 5 Real-time PCR system (Bio-Rad, CA, USA) following the manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Microbiology 2020Quote: ... 2,5 or 5 μL of the eluate were analysed by RT-qPCR using CFX96 Real-Time Thermal Cycler (Bio-Rad, Hercules, California, USA) as described elsewhere [76] with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... was electroporated into cells at 5×106 cells/0.4 ml under conditions of 220V and 950 µF using a Gene Pulser™ (Bio-Rad, Hercules, CA) and plated on DMEM containing 10% FBS.
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bioRxiv - Cell Biology 2021Quote: ... After three washes (5, 10, 15 minutes) the blot was incubated for 60 minutes with secondary Goat Anti-Rabbit-HRP (Bio-Rad, 1:7000) and Alexa 647 Goat-Anti-Mouse antibodies (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted 1:10 and 5 μl of the diluted cDNA was loaded in a total volume of 20 μl (SYBR Green Supermix (Bio-Rad 172-5124) and run on the Bio-RAD CFX Connect Real-Time System ...
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bioRxiv - Genomics 2022Quote: ... The new plate with droplets was sealed with a pierceable PCR foil at 180 °C for 5 seconds using PX1 PCR Plate Sealer (Bio-Rad, Cat. 1814000). The PCR reaction was then carried out using the C1000 Touch™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges, BIO-RAD 7324661), then dialyzed ...
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bioRxiv - Systems Biology 2022Quote: ... sample buffer (5 minutes at 95°C) and subjected to gel electrophoresis (4– 15% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and immunoblotting (polyvinylidene difluoride Transfer Membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
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bioRxiv - Developmental Biology 2022Quote: ... and up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 172-5038). Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were denatured for 5 min at 95°C and separated by SDS-PAGE using precast 10% polyacrylamide 10-well (Bio-Rad, #456-1034) or 18-well (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μl of each sample was taken out separately for measuring protein concentration using Bradford protein assay reagent (BIO-RAD, Hercules, CA, USA). 5% of the lysates were boiled with 2 x laemmli buffer (BIO-RAD ...
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bioRxiv - Bioengineering 2020Quote: ... and placed into a precooled (5 °C) real-time PCR instrument (C1000™ Thermal Cycler and CFX96™ Real-Time System; Bio-Rad). For the fluorescence detection ...
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bioRxiv - Plant Biology 2021Quote: ... from Eurofins Genomics (https://www.eurofinsgenomics.eu/) were mixed and melted at 95◻°C for 5◻min in TE buffer in a T100™ thermocycler (Bio-rad). Then the denatured oligonucleotides were allowed to anneal as the temperature was reduced by 1 °C every 20 sec ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...