Labshake search
Citations for Bio-Rad :
2101 - 2150 of 4753 citations for 8 9 Didehydro 3'N demethyl 9 deoxo 4'' 6 12 trideoxy 6 9 epoxy 3'N ethylerythromycin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... were separated by 8% acrylamide SDS-PAGE and transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer kit (Bio-Rad). 5% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... Aliquots of cell lysates (40-60 μg of protein) were subjected to SDS/PAGE on an 8 to 15% (vol/vol) acrylamide gel (MiniProtean; Bio-Rad) including PageRuler Prestained Protein Ladder (Thermo) ...
-
bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
-
bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
-
bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
-
bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
-
bioRxiv - Microbiology 2023Quote: ... Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380) diluted 1:10,000 in TBST ...
-
bioRxiv - Bioengineering 2023Quote: We used conjugated magnetic beads (6.5 μm) (Bio-Plex Pro Human Chemokine IL-8 / CXCL8 Set #171BK31MR2, BioRad, Hercules, CA, USA) for the binding assay ...
-
bioRxiv - Neuroscience 2022Quote: ... and then subjected to SDS polyacrylamide gel electrophoresis (PAGE) with 8% polyacrylamide and transferred to polyvinylidene difluoride (PVDF) membrane (BioRad Laboratories). The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were heated at 95°C for 5 minutes and cooled to room temperature before being separated on an 8% tris-glycine SDS-PAGE gel and transferred to a nitrocellulose membrane using a semi-dry transfer machine (Bio-Rad). Membranes were blocked in 5% skim milk dissolved in Tris-buffered saline with 0.1% Tween-20 (TBST ...
-
bioRxiv - Biochemistry 2023Quote: ... Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad). Following antibody incubation (anti-GFP ...
-
bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (20-30 μg) were resolved by 8 to 15% SDS PAGE MiniPROTEAN® TGX gels (Bio-Rad), from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad ...
-
bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
-
bioRxiv - Plant Biology 2020Quote: Forward and reverse new PCR primers for the 12 Zn transport-related genes and the two reference genes suitable for SYBR® Green II RT-qPCR assays (Biorad, USA) were designed (Table 2) ...
-
bioRxiv - Molecular Biology 2020Quote: Protein lysates were separated on 12% SDS polyacrylamide gels and transferred to PVDF membranes using the Trans-Blot Turbo System (BioRad, Feldkirchen, Germany). Equal protein loading and successful blotting was verified by Coomassie Brilliant Blue staining ...
-
bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and MgCl2 [12.5 mM]) and annealed by cooling the solution from 95 to 12 °C over 30 min in a thermal cycler (Bio-Rad, T100). Detailed descriptions for the assembly of individual gate complexes are described in the supplementary information ...
-
bioRxiv - Cancer Biology 2020Quote: ... The proteins were resolved by SDS-PAGE 12% self-cast gel and transferred onto polyvinylidene difluoride (PVDF) membrane using the Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) in transfer buffer (25 mM Tris ...
-
bioRxiv - Developmental Biology 2019Quote: ... Subsequent analysis was conducted on a 12% SDS-PAGE gel and blots were probed with either mouse α-V5 (SV5-Pk1, 1:1000, Bio-Rad), mouse α-HA (HA.11 ...
-
bioRxiv - Biochemistry 2021Quote: ... The treated lysates were mixed with the 2 × SDS-PAGE sample buffer with or without 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
-
bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred on a Nitrocellulose membrane for 12 minutes using the semi-dry turbo transfer western blot apparatus (Bio-Rad, USA). Post-transfer ...
-
bioRxiv - Microbiology 2020Quote: ... A total of 2.5-10 µg of each sample was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) through 12% Mini-PROTEAN® TGX™ Precast Gels (4561043, Biorad). After transferring the proteins onto a 0.2 µm Nitrocellulose membrane (Biorad ...
-
bioRxiv - Cell Biology 2021Quote: ... Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were run on denaturing sodium dodecylsulfate-polyacrylamide gel electrophoresis gels (12%) before transferring onto Immuno-Blot™ polyvinylidene fluoride membranes (Bio-Rad). The reference protein β-actin was used as a loading control.
-
bioRxiv - Immunology 2020Quote: ... Proteins were denaturated at 95°C for 5 minutes before separation on SDS-PAGE TGX 12% Stain-Free FastCast Acrylamide (Bio-Rad Laboratories). Proteins were then electro-transferred onto Trans Blot Turbo polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2020Quote: ... using AnykD or 12% polyacrylamide gels and transferred to PVDF membranes using the Transblot Turbo apparatus (Bio-Rad, Hercules, CA, United States). PVDF membranes were blocked for 1-hour in TBST with 5% milk ...
-
bioRxiv - Genomics 2020Quote: ... 5 µL of cDNA was pre-amplified in a 12-cycle PCR reaction using the Sso Advanced PreAmp Supermix (Bio-Rad, USA) in a 50 µL reaction ...
-
bioRxiv - Developmental Biology 2022Quote: ... lieberkuehni developmental stages were mixed in 2x Laemmli sample buffer with 10 % 2-β-mercaptoethanol and heated at 97 °C for 5 min before loading on a 12 % (w/v) TGX FastCast SDS/PAGE gel (Bio-Rad, USA). Membranes were blocked in 3 % (w/v ...
-
bioRxiv - Neuroscience 2022Quote: ... 40 mg of total protein was loaded onto a 12-15% polyacrylamide gradient gel (Mini-PROTEAN TGX Precast Mini Gel; Bio-Rad Laboratories) and transferred to a nitrocellulose membrane following separation ...
-
bioRxiv - Cell Biology 2019Quote: ... conjugates were analyzed by SDS-PAGE under reducing conditions on a 12% Tris-HCl polyacrylamide gel and stained with Coomassie Brilliant Blue R-250 (BioRad, Ontario, Canada). The migration distance in the gel relative to the blue dye front (Rf ...
-
bioRxiv - Zoology 2020Quote: ... An equal volume of each lysate was added to the designated lane on a 12% polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and separated by electrophoresis ...
-
bioRxiv - Microbiology 2021Quote: ... Protein extracts were subjected to SDS-PAGE for 90 min at 130 V at room temperature on 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad #4568046). Samples were transferred to nitrocellulose membranes using a BioRad Trans-Blot Turbo system (“standard sd” protocol) ...
-
bioRxiv - Physiology 2019Quote: ... 25 µg of protein extract per sample was separated on 12% acrylamide gels (#5678044, Criterion™ TGX Stain-Free™ Protein Gel, Biorad) and blotted on PVDF membranes (Immobilon-FL IPFL00010 ...
-
bioRxiv - Genetics 2021Quote: ... A total of 20 μg protein was separated with 12% (vol/vol) sodium dodecyl sulphate (SDS)-polyacrylamide gel and electrophoretically transferred onto a polyvinylidene difluoride membrane (Bio-Rad, L1620177). The membrane was soaked with 5% (w/v ...
-
bioRxiv - Plant Biology 2021Quote: ... 20 μl of eluted protein samples were separated on SDS gel (12%) and transferred to PVDF membrane (Immun-Blot® PVDF, Bio-Rad) through semi-wet blotting protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 20 μg of proteins per sample were resolved in 10 or 12% gels (TGX™ FastCast™ Acrylamide kit, Bio-Rad) via electrophoresis ...
-
bioRxiv - Cancer Biology 2022Quote: Whole cell extracts of NB4 and HT93 were prepared using urea lysis buffer and 30-60μg total protein was loaded on a denaturing 12%-polyacrylamide gel self-cast gel (Bio-Rad Laboratories, CA). Proteins were transferred to polyvinylidene fluoride (PVDF ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
-
bioRxiv - Microbiology 2022Quote: ... Laemmli buffer was added to samples.10 μL of samples were loaded onto 12 % Mini-P ROTEAN® TGX™ Precast Protein Gels (Biorad). Silver staining was carried out at room temperature for 1 h and gels were developed for 2 to 3 mins ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
-
ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of protein from each lysate were resolved on 10% or 12% sodium dodecyl sulphate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were then separated on 10-12% SDS-PAGE gels and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo™ transfer system (Bio-Rad). Membranes were blocked and primary antibodies were diluted in 2% BSA in Tris-buffered saline (TBS) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 µg of extracted proteins mixed with 2× SDS sample buffer were separated on 10% SDS PAGE gel (Mini PROTEAN TGX Precast Gel, 10%, 12-well comb; BioRad, 456-1035) and transferred to a nitrocellulose membrane (Amersham Protran Premium ECL 0.45 µm ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: A total protein amount of 100 μg for each sample was separated on 12% Criterion gels (Bio-Rad Laboratories, Hercules, Ca, USA), following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... Putative genomic deletions in the Fo-w and Fo-cn knockout lines were evaluated by amplifying 12 ng of gDNA in 50 μl PCR reactions using a C1000 Touch Thermal Cycler (BioRad, Berkeley, CA). All reactions were carried out to the manufacturer’s recommendations using Ex Taq polymerase (Takara Bio ...
-
bioRxiv - Bioengineering 2020Quote: ... Samples were denatured by incubating at 95°C for 10 minutes and loaded onto a denaturing 10% polyacrylamide gel with 8 M Urea in a Mini-PROTEAN® Electrophoresis System (BIO-RAD). Electrophoresis was performed at 240 V for 45 minutes ...