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Citations for Bio-Rad :
2051 - 2100 of 4753 citations for 8 9 Didehydro 3'N demethyl 9 deoxo 4'' 6 12 trideoxy 6 9 epoxy 3'N ethylerythromycin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... were separated on 12% (w/v) SDS-PAGE gels and transferred to an Immun-Blot low fluorescence-PVDF membrane (Bio-rad). The membrane was first blocked with Odyssey Blocking Buffer (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein (30 μg) was resolved on a 12% SDS-PAGE and transferred to a 0.45 μm nitrocellulose membrane (BioRad Cat# 1620115). The membranes were probed with anti-MAG ...
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bioRxiv - Immunology 2022Quote: 2 µg of GST or GST-IFITM3 protein was loaded into a 12% acrylamide Criterion XT Bis-Tris Precast Gel (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 15-30 μg of protein lysate was loaded and separated on 12% Tris-glycine SDS-PAGE gels and transferred to nitrocellulose membranes (catalog number 1620167, Bio-Rad) using Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2022Quote: ... Equal amounts of protein extracts were resolved by 10%-12% SDS-PAGE and electrotransferred onto a polyvinylidene membrane (Bio-Rad, USA). After blocking in Tris-buffered saline plus 5% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Developmental Biology 2022Quote: Protein extracts were separated on a 12% SDS gel with a 5% stacking gel and transferred to PVDF membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked using 5% milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS‒PAGE in 12% acrylamide gels and then transferred onto PVDF membranes using a Trans-Blot Turbo Transfer System (Bio-Rad) and PVDF transfer packs (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 15 µg of protein from each sample were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) (12%) and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad) for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: Formalin-fixed and paraffin-embedded 5μm sections were stained with hematoxylin and eosin (HE), DCT (Pep8h), SOX10 (clone EP268, Cell Marque, 383R-15) or CD3 (clone CD3-12, BioRad, MCA1477) antibodies using standard immunohistochemistry methods ...
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bioRxiv - Neuroscience 2023Quote: ... An equal amount of sample was loaded and separated on a 12% bis-tris gel and transferred to PVDF membranes (1620177 Bio-Rad) using a Bio-Rad cassette at 70 volts for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...
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bioRxiv - Biophysics 2024Quote: ... total proteins and biotinylated proteins were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Microbiology 2023Quote: Dilution series containing equal amounts of protein were prepared and separated on 12% Mini-PROTEAN TGX gels (BioRad, Hercules, CA, USA) by SDS-PAGE and then transferred onto 0.2 µM polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 µg protein were loaded on 12% SDS-PAGE gels and separated with a Mini protean III system (Bio-Rad). The proteins were transferred onto nitrocellulose membranes by electroblotting adopting standard protocols ...
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bioRxiv - Physiology 2024Quote: ... 5 ug (BAT) or 10 ug (ingWAT) of protein was separated on a 12% polyacrylamide gel (Mini-PROTEAN® TGXTM, BioRad). Proteins were then transferred to a PVDF membrane (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated by SDS-PAGE electrophoresis in 12% or 14% acrylamide gels, and transferred on nitrocellulose membranes (Amersham, 10600021) by semi-dry electrophoretic transfer (Bio-Rad). Accordingly with primary antibody datasheets ...
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bioRxiv - Microbiology 2023Quote: ... run on a 12% SDS‒PAGE gel and transferred onto a PVDF membrane using a Trans-Blot Turbo electrophoretic transfer cell (Bio-Rad) at 25 V for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The digestion products were analyzed by 12-15% SDS-PAGE and stained with Coomassie blue or silver (Silver stain plus kit, Bio-rad). Images were taken using a Chemidoc imaging system (Bio-Rad) ...
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bioRxiv - Developmental Biology 2023Quote: ... Beads were collected after washing thrice with 1× RIPA buffer and separated on a 12% denaturing SDS polyacrylamide gel followed by transfer onto Immuno-blot PVDF membranes (Bio-Rad). Mouse anti-arm antibody at 1:1500 dilution or rabbit anti-FLAG antibody at 1:1000 dilution and goat anti-rabbit IgG–AP conjugate at 1:2000 or goat anti-mouse IgG-AP conjugate at 1:200 dilution (Molecular Probes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... kit with 0.25 µl cDNA in a 12 µl final volume in Hard-Shell Thin-Wall 384-Well Skirted PCR Plates (Bio-rad). Gene-specific primers were designed using Primer3 software to obtain a 100- to 150-bp product and purchased from Sigma-Aldrich (Saint Quentin ...
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies) and in Criterion cells (Bio-Rad) using respective adapters (LifeTechnologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded on SDS-polyacrylamide gels (8 to 10 %) and transferred to nitrocellulose membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were saturated for 1 h with TBST buffer (150 mM NaCl ...
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bioRxiv - Physiology 2019Quote: For serum fetuin-A analysis murine serum was separated by sodium dodecyl sulphate polyacrylamide gel elecrophoresis using mini gels (10% acrylamide, 5 × 8 × 0.1 cm3, BioRad, Hercules, USA). Protein transfer onto nitrocellulose membrane was by semi-dry electroblotting (Owl HEP-1 ...
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bioRxiv - Genetics 2019Quote: ... Samples containing 10 μg of total RNA were separated on 8M urea-containing 8% polyacrylamide gels followed by electroblotting to Zeta-probe membranes (Bio-Rad). The blots were sequentially probed for and using 32P-labeled oligonucleotides (S7 Table) ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were separated by electrophoresis on 8% SDS-PAGE gels and subsequently transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blots were blocked with 5% milk in TBST (TBS + 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Western blot was performed using 8 or 10% mini gels followed by wet transfer (1 h, 100 V) to Western grade nitrocellulose (Bio-Rad). Blots were incubated in Intercept blocking buffer (LI-COR ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated the supernatant on a 3-kDa cutoff centrifugal filter unit (Amicon) and loaded samples of supernatant on a 8-16% SDS-PAGE TGX Stain-Free gels (Bio-Rad) (SI Figure 1c).
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bioRxiv - Cell Biology 2021Quote: ... 10 to 30μg of total protein were subjected to 8% or 10% SDS-PAGE gels and transferred electrophoretically onto PVDF membrane by a semi-dry blotting system (Bio-Rad). The membrane was blocked in 5% fat-free milk/Tris-buffered saline for 1 hour and incubated with primary antibodies at 4°C overnight followed by horseradish peroxidase conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Neuroscience 2021Quote: ... were added to the 8 channel droplet generation cartridge according to manufacturer instructions and this cartridge was placed in the QX200 droplet generator (Bio-Rad). Then ...
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bioRxiv - Immunology 2021Quote: ... and 2D-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) replicates performed on 11cm 8-16% (w/v) polyacrylamide/Tris-HCl gels (Bio-Rad) using a Criterion Dodeca Cell (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Lysates (20–30 μL) were run on 8–10% polyacrylamide gels and transferred to PVDF membranes (Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were blocked with 5% BSA in TBS (TBS ...
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bioRxiv - Microbiology 2020Quote: ... Flow cytometric staining and analysis was done as described [8] and acquired on ZE5 cell analyzer (Bio-Rad, Hercules, CA, USA). Analysis was performed using Flow Jo software.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips pH 5-8 for CDK4 (BioRad, Hercules, CA, USA) or pH 3–10 for CDK2 (Amersham Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... unless otherwise stated) were subjected to sodium docedyl sulfate-polyacrylamide (SDS-PAGE) electrophoresis on an 8% acrylamide gel (MiniProtean, Bio-Rad) including PageRuler Plus Prestained Protein Ladder (Thermo) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: PCR Products were separated on a 0.8% agarose gel and visualized after staining with DNA Stain Clear G (SERVA, Heidelberg) on a ChemiDoc imaging system (Biorad, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... 10nM of templates with each promoter type were added to TXTL reactions and incubated at 30 °C for 8 hours (T100 Thermal Cycler, Bio-Rad). Post-incubation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: ... or 1:8 (for all other quantifications) and 2ul was used to quantify gene expression using iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were separated by 8% SDS-PAGE and Western blotted onto a PVDF membrane (semidry transfer, 15 V, 45 min, Trans blot Bio-Rad). The membranes were incubated overnight at 4°C in blocking solution 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... and treated with or without heat denaturation (96°C, 8 min) and ran on a TGX stain-free precast gel (Bio-Rad) for 38 min at 200 V ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 ng RNA (RIN>8) was transcribed to cDNA using the iScript first strand cDNA synthesis kit (Bio-Rad 1708891). qPCR was carried out on a 384-well Quantstudio Flex system (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of diluted (1:8) cDNA were loaded for qPCR analysis along with the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725272) and 0.5 μM forward and reverse primers for target genes (listed below) ...