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Citations for Bio-Rad :
2001 - 2050 of 6633 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Immunoblots were developed using the SuperSignal West Pico chemiluminescent substrate (Pierce,ThermoScientific) and analyzed using the ChemiDoc XRS + imagingsystem (Bio-Rad, http://www.bio-rad.com/). Immunoblots in the linear range of detection were quantified using Quantity One software (Bio-Rad Laboratories).
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bioRxiv - Immunology 2022Quote: ... diluted to 1:5,000 in 1X TBST 5% milk powder for 1 hour before visualisation with Clarity™ Western ECL Substrate (Bio-Rad, Hercules, California, US) using a Syngene G ...
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bioRxiv - Bioengineering 2019Quote: ... the protein bands were visualized upon few minutes of incubation of the membranes with Bio-Rad Clarity Western ECL Substrate (Bio-Rad#1705061, Cressier, Switzerland). Blots were imaged using ChemiDoc imaging system for chemiluminescence assays with 0.1 s exposure ...
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bioRxiv - Neuroscience 2019Quote: ... 1:5,000 for β-actin) and the Clarity Western ECL system served as the substrate for the peroxidase enzyme (Cat# 1705060, Bio-Rad, Hercules, CA). For semi-quantitative analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... Chemiluminescent detection was performed using film or a VersaDoc CCD camera with Clarity™ Western ECL Substrate and horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA) the next day ...
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bioRxiv - Cell Biology 2023Quote: ... They were then washed thrice with TBST (5 minutes/wash) at room temperature and signal developed for one minute using Clarity™ Western ECL substrate (Bio-Rad, Cat #: 1705060) or Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... the incubated membranes were washed again three times with 1x TBST for 10 minutes each at RT and visualised with Clarity Western ECL Substrate (BIO-RAD, Cat. #170-5060).
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bioRxiv - Developmental Biology 2023Quote: ... The membranes were washed with TBST three times and then incubated with a secondary antibody for 1 h before using Luminol/enhancer solution and ClarityTM Western ECL Substrate (Bio-Rad Laboratories, Inc. US). Western blot images were scanned by using Gel Doc XR system (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... The blots were developed using the Luminata Forte Western Chemiluminescent HRP Substrate (MilliporeSigma, Burlington, MA, USA) and visualized on a ChemiDoc Imaging System (Bio-Rad, Hercules, CA, USA). Densitometry analysis was performed using Image Lab software version 6.0 (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and loaded on 10% gels (Bio-Rad) for electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... and 72°C for 1 min in a C100 thermocycler (BioRad, Hercules, CA, USA). 5 μL of this reaction was removed and used for a qPCR side reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... then stored at −20°C in 1X Laemmli sample buffer (Bio-Rad 161-0747) with 10% betamercaptoethanol (BME ...
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bioRxiv - Physiology 2019Quote: ... and blocked for 24 hours at 4°C in 5% blotting-grade blocker (BioRad). Blots were incubated in primary OXPHOS antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Western blotting was done at 4°C using Mini Trans-Blot Cell Assembly (BioRad) at 25 V ...
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bioRxiv - Microbiology 2021Quote: ... 67.1 and 70°C for 10 min in a C1000 TouchTM Cycler (Bio-Rad) and measuring the enzymatic activity with the Mu-NANA assay ...
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bioRxiv - Biophysics 2020Quote: ... Detergents were removed by incubation overnight at 4⁰ C with Bio-Beads (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... and annealing at 60 °C for 30 s in a CFX96 System (Bio-Rad). The primer sequences are listed in supplementary Table S1.
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bioRxiv - Immunology 2022Quote: ... for 2 hours at 4 °C using Mini-trans blot wet tank transfer (BioRad). After transfer ...
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bioRxiv - Cancer Biology 2021Quote: ... Electrophoresis was performed at 14 °C in 0.9% pulse field-certified agarose (Bio-Rad) using Tris-borate-EDTA buffer in a Bio-Rad Chef DR III apparatus (9 h ...
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bioRxiv - Microbiology 2020Quote: ... and a final extension of 72°C for 5 minutes (C1000, BioRad Labs., Inc.). Samples were immediately removed upon completion of amplification and stored at -20°C until the emulsion was broken as described above.
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bioRxiv - Microbiology 2020Quote: ... and a final extension of 72°C for 5 minutes (C1000, BioRad Labs., Inc.). Samples were immediately removed upon completion of amplification and stored at -20°C until the emulsion was broken.
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bioRxiv - Microbiology 2021Quote: ... at 37 °C as previously described (84) and included reduction/alkylation with dithiothreitol (BioRad) and iodoacetamide (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... and the samples were placed in a preheated 37°C thermal cycler (BioRad T100) to digest for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4 °C hold in a thermocycler (model C1000 Touch Thermal Cycler, Bio-Rad Laboratories - Life Sciences ...
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bioRxiv - Bioengineering 2022Quote: ... stored at 4°C in the dark until acquisition on ZE5 cytometer (Bio-Rad). For analysis of mouse samples ...
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bioRxiv - Molecular Biology 2022Quote: ... for passive rehydration overnight at 20 °C in a Protean IEF Cell equipment (BioRad). Isoelectric focusing (IEF ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 hour at 37 °C before resolved on 10% TBE PAGE gels (BioRad) run at 100 volts for 2 hours ...
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bioRxiv - Biophysics 2022Quote: Analytical SEC experiments were carried out at 4 °C using an NGC Quest (BioRad) chromatography system ...
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bioRxiv - Cell Biology 2023Quote: ... and then heated to 95 °C in the presence of 2x Laemmli buffer (BioRad) to elute proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... then 4°C hold in a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA). cDNA was stored at −20°C.
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bioRxiv - Immunology 2023Quote: ... and then heated at 98°C in Laemmli Sample Buffer (Bio-rad, Cat# 1610747). Samples were run in a SDS-PAGE gel and lipid-interacting proteins were detected by immunoblotting using HA-tag antibody (Proteintech Group ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C using the Mini Trans-Blot Cell (Bio-Rad). After the transfer ...
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bioRxiv - Immunology 2024Quote: ... Reaction was performed for 2h at 37°C in nuclease-free microcentrifuge tubes (BioRad). A260/295 was measured with a nanodrop (company ...
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bioRxiv - Neuroscience 2023Quote: Samples were denatured at 95°C for 10 min in Laemmli buffer (Bio-Rad) with 2% β-mercaptoethanol and the indicated amount of proteins were resolved by SDS–PAGE and transferred onto PVDF membrane (Immobilon-P ...
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bioRxiv - Microbiology 2024Quote: ... The assays were incubated for 2 hours at 37 °C in a thermocycler (Biorad). Samples were then filtered through 3 kDa filter plates and derivatized with 4,5-dimethoxy-1,2-phenylenediamine hydrochloride (DMB) ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were treated with ECL Prime Western Blotting reagent (Amersham, UK) or Clarity Western ECL Substrate and exposed with a ChemiDoc Touch Imaging System (Bio-Rad, Marnes-la-Coquette, France). The band density was quantified with Image Lab software (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Thermo Fischer Scientific, Waltham, MA, USA) and the Chemidoc Touch Imaging system (Bio-Rad, Hercules, CA, USA). Quantification of band intensities was performed using ImageJ and normalized to the loading control hsp90.
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times for 10 mins with TTBS and once for 10 mins with TBS before developing it with the Clarity Western ECL Substrate (Bio-Rad Laboratories, cat. #170-5061) and visualizing it using the Bio-Rad ChemiDoc MP Imaging system ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoreactive proteins were detected using SuperSignal West Dura Extended Duration Substrate (34076, Thermo, Rockford, IL, USA) and detected using a ChemiDoc MP Imaging system (Bio-Rad Laboratories, Hercules, CA, USA). The band intensity was densitometrically evaluated using Image Lab software (Version 5.2 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Molecular Biology 2019Quote: ... A chemiluminescent signal was generated with addition of Clarity™ Western ECL substrate and imaged in a ChemiDoc™ Touch Imaging System (Bio-Rad, Hercules, CA, USA). Image Lab software (Version 5.2.1 ...
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bioRxiv - Physiology 2022Quote: ... The PVDF membranes were treated with a chemiluminescent substrate (ThermoFisherScientific, Waltham, MA, USA) and imaged using a ChemiDoc™ Imaging system and Image Lab™ software (Bio-Rad, Hercules, CA, USA). Total protein was assessed via BLOT-FastStain (G-Bioscience ...
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bioRxiv - Physiology 2023Quote: ... The antibody-probed PVDF membranes were treated with chemiluminescent substrate (ThermoFisherScientific, Waltham, MA, USA) and images acquired with a ChemiDoc™ Imaging system and Image Lab™ software (Bio-Rad, Hercules, CA, USA). Total protein was measured via BLOT-FastStain (G-Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... 60 °C for 1 min for 40 cycles and analyzed using 7900HT SDS software (Biorad). Each run consisted of triplicate technical repeats per experiment ...
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bioRxiv - Microbiology 2020Quote: ... and gradually cooled to 25°C over 45 minutes in a S1000 Thermocycler (Bio-Rad) and stored at −20°C ...
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bioRxiv - Cell Biology 2021Quote: ... in a 20 μl reaction on a thermal cycler (C-1000 Touch; Bio-Rad Laboratories). Product sizes were controlled by DNA gel electrophoresis and the melt curves were evaluated using CFX Manager (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: The concentrations of the 37°C samples were determined with a DC Protein Assay (BioRad). A volume corresponding to 50 μg of the 37°C sample was used for further analysis ...
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bioRxiv - Neuroscience 2021Quote: ... heated at 70°C for 10min and loaded into 4-20% precast gels (Bio-rad) for electrophoresis (130V ...
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bioRxiv - Biochemistry 2020Quote: ... SEC purification was performed at 4°C on an Enrich SEC 650 column (Bio-Rad) and sample eluted in SEC buffer (20 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysate was clarified by centrifugation at 4 °C and protein quantified using bradford (Bio-Rad) assay ...