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Citations for Bio-Rad :
1901 - 1950 of 6633 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were used for flow cytometric analysis after cells were stained with Alexa647-conjugated rat anti-mouse CD204 antibody (clone 2F8; Bio-Rad Laboratories GmbH ...
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Pathology 2021Quote: ... The sections were then incubated in blocking buffer (10% goat serum; 1 hour) followed by incubation with either rat anti-F4/80 antibody (Bio-Rad) diluted 1:500 or rat anti-CD68 antibody (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression levels of 85 genes were assessed using the PrimePCR Antibacterial Response (SAB Target List) R384 rat plate (Catalog #10047065; Bio-Rad) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... tissue sections were blocked in 10% normal goat serum for 1 h at room temperature and incubated in rat anti-mouse CD4 (1:200; MCA2691; Bio-Rad) or CD8a (Biolegend ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Neuroscience 2020Quote: ... the BCIP/NBT substrate was added and the results were analyzed using the GelDoc EZ imager and Image Lab software (BioRad, Temse, Belgium). TBS buffer was used for washing between the steps.
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bioRxiv - Neuroscience 2021Quote: ... and the Clarity Western enhanced chemiluminescence (ECL) system served as the substrate for the peroxidase enzyme (Cat# 1705060, Bio-Rad, Hercules, CA). A list of antibodies and reagents used for the dot blot experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated with primary antibody and horseradish peroxidase–conjugated secondary antibody, and then proteins were detected using the Pierce™ ECL Western Blotting Substrate (Thermo, #32209) by BIO-RAD ChemiDoc™ XRS+ system ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then washed 3 times in PBS-T followed by a final wash in PBS before signal development in Clarity Western ECL substrate (Bio-rad laboratories) and visualization on a ChemiDoc MP imager.
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bioRxiv - Biochemistry 2020Quote: ... The presence of the secondary antibody was confirmed using the Pierce™ECL Plus Western blotting substrate (ThermoScientific, USA) and viewed using the ChemiDoc (BioRad, USA).
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bioRxiv - Bioengineering 2021Quote: ... each wash was 10-min long) and developed using the Clarity Western ECL substrate (Bio-Rad, Hercules, CA, USA, cat. #170-5060). Immunoreactive bands were visualized with ChemiDoc™ XRS Imaging System (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The lectin blots were developed using SuperSignal™ West Pico Plus Chemiluminescent substrate (Thermo Fischer Scientific) and the images acquired using a ChemiDoc Imaging system (Bio-Rad). The band intensities were analyzed using the ImageLab (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no. 1705061, Bio-Rad, Gladesville ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected by chemiluminescence using the Clarity™ Western ECL Substrate coupled to a ChemiDoc XRS+(Bio-Rad, Hercules, CA, USA). Quantification of Western blots relied on ImageJ software [42].
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG HRP conjugated secondary antibody was used to detect the protein bands by chemiluminescence (Clarity ECL substrate, Cat no.170-5061, Biorad, California, USA).
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bioRxiv - Neuroscience 2022Quote: ... macrophages were plated in a 24-well plate containing cover slips and incubated with the Magic Red cathepsin B substrate (1/250) (Bio-Rad, ICT938) for 2h ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were incubated in secondary antibody for one hour at room temperature (anti-rabbit; Amersham, #NA934) (anti-mouse; Amersham, #NA931) and visualized with Clarity Western ECL Substrate (Bio-Rad, #1705061) on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at RT and after incubation the blots were developed in an ECL detection solution (Clarity Max ECL substrate, Bio-Rad Laboratories) and signal was detected using ChemiDoc (BioRad Laboratories).
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bioRxiv - Developmental Biology 2021Quote: ... Following 4x 10 min washes with PBS-T the membrane was incubated for 1 min with Clarity Western ECL Substrate (170-5060, Bio-Rad Laboratories) and imaged with Amersham™ Imager 600 (GE ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies were added according to manufacturer’s protocol and membranes were imaged using chemiluminescence reagent Clarity Western ECL Substrate (BioRad laboratories, Hercules, CA) and a BioRad ChemiDoc XRS system ...
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bioRxiv - Bioengineering 2020Quote: ... The membranes were developed using ECL western blotting substrate (Pierce Biotechnology, Rockford, IL) and scanned by ChemiDoc XRS+ system (BioRad, Hercules, CA). Western blots were quantified using Image Lab 6.0.1 software (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed again with TBST buffer for 5 times X 5 min and developed using Clarity Western ECL substrate (Bio-Rad, 1004384863). Images were captured on a Luminescent Image Analyzer (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (Cytiva) and images were acquired on ChemiDoc Touch (Bio-Rad Laboratories). The acquired images were processed and quantified using Image Lab software (Bio-Rad laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Each membrane was then incubated with the appropriate HRP-conjugated secondary antibodies in TBS-T and visualized by using the Enhanced chemiluminescence substrate (BioRad 170-5060) and ChemiDoc (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed as previously mentioned and the antigen-antibody complex was revealed by Clarity Enhanced Chemiluminescence Blotting Substrate (Bio-Rad, #1705060) using the iBright™ FL100 Imaging System (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were finally developed in a 1:1 mix of Peroxide Solution and Luminol from the SuperSignal® West Pico Chemiluminescent Substrate (#34078, ThermoFisherScientific) and visualized in a ChemiDocTM Touch Gel Imaging System Transiluminator (#1708370, Bio-Rad). Serial dilutions of commercial LDL (#SAE0053 ...
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bioRxiv - Microbiology 2023Quote: ... The horse-radish peroxidase conjugated to the secondary antibody was detected by incubating the membrane with 2 mL chemiluminescence substrate (Bio-Rad, 1705062) for five minutes and then imaged using a ChemiDoc XRS+ imager (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... After the membranes were washed 3x during 5 minutes in TBST and the chemiluminescence was detected using Clarity™ Western ECL Substrate (Bio-Rad) and ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were then subjected to peroxidase-based detection by using enhanced chemiluminescence (ECL) detection reagent (Clarity Max™ Western ECL Substrate, Bio-Rad), and the chemiluminescent signals were visualized by ChemiDoc Touch System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were added Pierce™ ECL Western Blotting Substrate (Thermo Fischer) and chemiluminescent signal was revealed using a ChemiDoc MP instrument (Bio-Rad) with a exposure time of 800 s ...
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bioRxiv - Microbiology 2019Quote: ... 98°C for 10 min) with C1000 Touch thermal cycler (Bio-Rad). Fluorescent signal was detected using QX200 Droplet Reader (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated (4°C, overnight) with 5 % milk powder (BioRad) in TBST ...
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bioRxiv - Neuroscience 2019Quote: ... 72°C for 2’ in a T100™ Thermal Cycler (Bio-Rad) followed by long-term storage at −20°C ...
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bioRxiv - Immunology 2019Quote: ... Cells were analysed at 4°C using a ZE5 Flow cytometer (BioRad) set to sample wells until running dry ...
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bioRxiv - Biophysics 2022Quote: ... followed by 5 min at 60 °C in a thermocycler (Biorad T100) with the lid heated to 105 °C.
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bioRxiv - Biochemistry 2021Quote: ... and electro-blotted onto PVDF membranes (Biorad: 100 V, 2h, 4°C). The transferred proteins were then renatured by progressively reducing the guanidine-HCl concentration ...
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bioRxiv - Immunology 2022Quote: ... before being lysed at 90 °C in a thermal cycler (BioRad T1000) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... then placed in 4% normal goat serum in 0.01M PBS blocking solution for 60 minutes with agitation followed by incubation with rat anti-CD68 (1:500; BioRad, cat no. MCA1957GA) and rabbit anti-MBP (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were assessed using Bio-Rad BioPlex assays in both plasma and PFC homogenates (Th1/Th2 rat cytokines Bio-Rad, #171k1002M), according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Biophysics 2021Quote: ... A standard lane containing a small amount of the DNA substrate used in the packaging reaction allowed for quantification of packaged DNA using the Gel ChemiDoc MP imaging system (Bio-Rad, Hercules, CA). In the DNA packaging experiments involving doping with inactive subunits ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
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bioRxiv - Developmental Biology 2020Quote: ... followed by Super Signal@ West Femto chemiluminescent substrate (Pierce, Rockford, IL, USA) and the products were recovered by Laser 4000 chemiluminescent system (Bio-Rad, CA, USA). Finally ...
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bioRxiv - Cell Biology 2019Quote: ... Western Blotting Substrate (Amersham-Pharmacia, Piscataway, NJ, USA) and image acquisition that was carried out using the ChemiDoc System (Bio-Rad, Milan, Italy). Western blot analysis were repeated 4 times.
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bioRxiv - Biophysics 2019Quote: ... The relative amount of present HRP was detected by adding 60 µL/well DPBS + 1 mM CaCl2 and 20 µl/well HRP substrate (Bio-Rad, 170-5060). The plate was incubated for 10 minutes at room temperature before recording of luminescence on an EnVision plate reader (Perkin Elmer).